The Korean Journal of Mycology (한국균학회지)

The Korean Society of Mycology (한국균학회)
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Unrecorded Fungi Isolated from Fire Blight-controlled Apple Orchard Soil in Korea

  • Soo Young Chi (Department of Microbiology, College of Science and Technology, Dankook University) ;
  • Jun Woo Cho (Department of Microbiology, College of Science and Technology, Dankook University) ;
  • Hyeongjin Noh (Department of Microbiology, College of Science and Technology, Dankook University) ;
  • Minseok Kim (Department of Microbiology, College of Science and Technology, Dankook University) ;
  • Ye Eun Kim (Plant Quarantine Technology Center, Animal and Plant Quarantine Agency) ;
  • Seong Hwan Kim (Department of Microbiology, College of Science and Technology, Dankook University)
  • Received : 2023.11.14
  • Accepted : 2023.12.26
  • Published : 2023.12.31
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Abstract

To explore fungal diversity in orchard soil where fire-blighted apple trees are buried, we collected soil samples from apple orchards in Chungju, Korea. Fungal isolates were obtained from DG18 agar and identified at the species level based on morphological features and phylogenetic analyses. The colony characteristics and microstructures were examined using a light microscope and a scanning electron microscope after culturing on potato dextrose agar (PDA), malt extract agar (MEA), Czapek yeast agar (CYA), and oatmeal agar (OA) The PCR-amplified products of the ITS1-5.8S-ITS2 region and 28S large subunit of the nuclear ribosomal RNA gene, as well as partial sequences of the β-tubulin, calmodulin, and translation elongation factor 1-α genes were sequenced and analyzed phylogenetically. Seven previously unknown fungal species were explored in Korea. All samples, including Aspergillus aureolatus, Botryotrichum atrogriseum, Dactylonectria novozelandica, Fusarium denticulatum, Paecilomyces tabacinus, Sarcopodium tibetense and Talaromyces stollii, had ascomycetes. Herein, we report their descriptions and features.

Keywords

Acknowledgement

This work was supported by grants from the National Institute of Biological Resources (NIBR), funded by the Ministry of Environment (MOE), and by the Cooperative Research Program for Agriculture Science & Technology Development (Project No. PJ016242); RDA, and the Animal and Plant Quarantine Agency, Republic of Korea.

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