The Korean Journal of Mycology (한국균학회지)

The Korean Society of Mycology (한국균학회)
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Characteristics of Lentinula edodes Fruiting Bodies Formed by Mono-mono Hybridization Using Mating Type Analysis

교배형 분석을 활용한 표고 교배균주 육성 및 자실체 발생 특성

  • Yeongseon Jang (Forest Microbiology Division, Department of Forest Bio-resources, National Institute of Forest Science) ;
  • Mi-Jeong Park (Forest Microbiology Division, Department of Forest Bio-resources, National Institute of Forest Science) ;
  • Eunjin Kim (Forest Microbiology Division, Department of Forest Bio-resources, National Institute of Forest Science) ;
  • Min-Jun Kim (Forest Microbiology Division, Department of Forest Bio-resources, National Institute of Forest Science) ;
  • Yeun Sug Jeong (Forest Microbiology Division, Department of Forest Bio-resources, National Institute of Forest Science) ;
  • Kang-Hyeon Ka (Forest Microbiology Division, Department of Forest Bio-resources, National Institute of Forest Science)
  • 장영선 (국립산림과학원 산림생명자원연구부 산림미생물연구과) ;
  • 박미정 (국립산림과학원 산림생명자원연구부 산림미생물연구과) ;
  • 김은진 (국립산림과학원 산림생명자원연구부 산림미생물연구과) ;
  • 김민준 (국립산림과학원 산림생명자원연구부 산림미생물연구과) ;
  • 정연석 (국립산림과학원 산림생명자원연구부 산림미생물연구과) ;
  • 가강현 (국립산림과학원 산림생명자원연구부 산림미생물연구과)
  • Received : 2023.11.09
  • Accepted : 2023.12.15
  • Published : 2023.12.31
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Abstract

In this study, dikaryotic strains of Lentinula edodes were generated by mono-mono hybridization using mating type analysis and their fruiting body characteristics were investigated. Approximately 100 monokaryotic strains were isolated from the basidiospores of Sanbaekhyhang, and homokaryotic strains were isolated from Chungheung 1ho using protoplast isolation and regeneration. Their mating types were evaluated and a total of 60 dikaryotic strains were hybridized. Using these strains, fruiting bodies were produced and their characteristics were examined after cultivation on sawdust media. The results indicated that the rate of hybridization was 100% and that 55 of 60 strains formed fruiting bodies. These showed normal pileus and gill structures; however,10 strains also produced fruiting bodies with abnormal pileus and gill structures. The weight and size of the fruiting bodies differed depending on the strains. Overall, further studies are needed for predicting the characteristics of hybridized strains based on their parental strains.

본 연구에서는 교배형 분석을 활용하여 표고의 교배균주를 육성하고 그로부터 발생한 자실체의 특성을 알아보고자 하였다. 단핵균주 분리에는 산백향과 청흥1호 품종을 이용하였는데, 산백향은 버섯으로부터 100여개의 단포자를 분리하였으며, 청흥1호는 원형질체 분리 및 재생과정을 통해 단핵균주를 얻었다. 이렇게 얻는 단핵균주는 우선 교배형 분석을 실시하였으며, 교배 가능한 조합을 확인하고 총 60개의 균주를 교배하였다. 톱밥재배는 2 kg 사각블럭배지를 이용하였으며, 배양 120일 후에 버섯 발생처리를 하였다. 그 결과 60균주의 교배성공률은 100%에 이르렀으며, 그 중 55개인 91.7%의 균주에서 버섯이 발생하였다. 모든 균주에서 정상적인 버섯이 발생하였으며, 그 중 10개 균주에서는 주름살을 온전하게 형성하지 않는 기형버섯도 발생하는 것을 확인하였다. 버섯의 형태적인 특성은 매우 다양하게 나타났으며, 이러한 특성을 사전에 파악하기 위해서는 교배모본의 특성을 평가할 수 있는 연구가 더 필요하다.

Keywords

Acknowledgement

This study was supported by grant from the general project (FP0800-2023-01) of National Institute of Forest Science, Republic of Korea.

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