The Korean Journal of Mycology (한국균학회지)

The Korean Society of Mycology (한국균학회)
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First Record of Four Yeast Strains Isolated from Freshwater in Korea

국내 담수환경에서 분리된 국내 미기록 효모 4종 보고

  • Yoosun Oh (Fungi Research Team, Nakdonggang National Institute of Biological Resources) ;
  • Wonsu Cheon (Fungi Research Team, Nakdonggang National Institute of Biological Resources) ;
  • Hye Yeon Mun (Fungi Research Team, Nakdonggang National Institute of Biological Resources) ;
  • Jaeduk Goh (Fungi Research Team, Nakdonggang National Institute of Biological Resources)
  • 오유선 (국립낙동강생물자원관 미생물연구실 균류연구팀) ;
  • 천원수 (국립낙동강생물자원관 미생물연구실 균류연구팀) ;
  • 문혜연 (국립낙동강생물자원관 미생물연구실 균류연구팀) ;
  • 고재덕 (국립낙동강생물자원관 미생물연구실 균류연구팀)
  • Received : 2023.11.17
  • Accepted : 2023.12.07
  • Published : 2023.12.31
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Abstract

Freshwater ecosystems are significant habitats of fungi including yeasts. This study aimed to isolate and characterize wild yeasts from freshwater environments in Korea. The yeast isolates were identified by using the D1/D2 domains of the 26S rDNA regions. We identified four strains, Candida viswanathii (NNIBRFG39781), Curvibasidium cygneicollum (NNIBRFG49003), Oberwinklerozyma silvestris (NNIBRFG39803) and Vishniacozyma foliicola (NNIBRFG6120). These yeasts had not previously been recorded in Korea. We investigated the morphological and cultural characteristics of these yeasts. All of them grew on YPD, PD and YM media. Candida viswanathii (NNIBRFG39781), O. silvestris (NNIBRFG39803) and V. foliicola (NNIBRFG6120) grew in YPD medium containing glucose and in pH range of 4-8. Curvibasidium cygneicollum (NNIBRFG49003) grew well low temperature compared to others and slowly.

본 연구는 국내 담수환경으로부터 야생효모를 분리하여 특성을 분석하였다. 분리된 효모의 26S rDNA의 D1/D2부위 염기서열을 이용하여 동정을 하였다. 결과적으로 C. viswanathii (NNIBRFG39781), C. cygneicollum (NNIBRFG49003), O. silvestris (NNIBRFG39803), V. foliicola (NNIBRFG6120) 등 4종의 국내 미기록 야생효모들을 동정하였다. 이들 미기록 효모는 모두 출아에 의하여 영양생식을 하였고 PD, YM, YV 배지에서 잘 자랐다. C. viswanathii (NNIBRFG39781) 균주는 의균사를 형성하였으며 고온에서도 생육하였다. 또한 C. viswanathii (NNIBRFG39781), O. silvestris (NNIBRFG39803), V. foliicola (NNIBRFG6120) 균주는 20% glucose를 함유한 YPD 배지에서 생육하는 내당성을 보였으며 C. cygneicollum (NNIBRFG49003)와 O. silvestris (NNIBRFG39803) 균주는 NaCl을 함유한 배지에서는 생육하지 못하였다. 담수환경은 효모를 포함한 균류의 다양성이 높은 환경으로 지속적 연구가 필요하다. 본 연구는 향후 담수환경의 생물다양성 연구에 기초자료로 활용될 것이다.

Keywords

Acknowledgement

This work was supported by a grant from the Nakdonggang National Institute of Biological Resources (NNIBR), funded by the Ministry of Environment (MOE) of the Republic of Korea (NNIBR202301106).

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