The Korean Journal of Mycology (한국균학회지)

The Korean Society of Mycology (한국균학회)
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First Report of Pyrenophora tritici-repentis in Wheat (Triticum aestivum L.) in Korea and in vitro Selection of an Effective Fungicide

  • Min-Hye Jeong (Department of Plant Medicine, College of Life Science and Natural Resources, Sunchon National University) ;
  • Eu Ddeum Choi (Pear Research Institute, National Institute of Horticultural & Herbal Science) ;
  • Seol-Hwa Jang (Department of Plant Medicine, College of Life Science and Natural Resources, Sunchon National University) ;
  • Sang-Min Kim (Crop Foundation Research Division, National Institute of Crop Science, Rural Development Administration) ;
  • Sook-Young Park (Department of Plant Medicine, College of Life Science and Natural Resources, Sunchon National University)
  • Received : 2023.08.16
  • Accepted : 2023.11.08
  • Published : 2023.12.31
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Abstract

Tan spot, caused by Pyrenophora tritici-repentis, is a major foliar disease in wheat worldwide. In April 2021, tan spot symptoms were observed in a commercial wheat field in Suncheon, Jeonnam Province, Korea, with over 5% of the wheat leaves exhibiting symptoms. These symptoms included oval-shaped tan necrosis surrounded by a bright halo. The three representative isolates exhibited irregular mycelial growth on V8-potato dextrose agar and produced pseudothecia. Based on the concatenated sequence datasets of four multi-genes, including the internal transcribed spacer, large subunit ribosomal RNA, glyceraldehyde-3-phosphate dehydrogenase, and RNA polymerase II second-largest subunit genes, phylogenetic analysis revealed that these three isolates clustered in the same clade as P. tritici-repentis. Results of pathogenicity test indicated that the initial symptoms appeared 5 days post-inoculation (dpi), with typical tan spot symptoms developing at 7 dpi. The pathogen was successfully re-isolated from the symptomatic tissues, thus fulfilling Koch's postulates. Furthermore, we selected three fungicides that effectively inhibited the mycelial growth of P. tritici-repentis by more than 90% in vitro. To the best of our knowledge, this is the first report of tan spot disease in wheat in Korea.

Keywords

Acknowledgement

This study was supported by a grant from the Rural Development Administration (grant number: PJ014995042023).

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