DOI QR코드

DOI QR Code

Occurrence of Gray Mold in Wasabi Caused by Botrytis cinerea in Korea

  • Received : 2023.08.23
  • Accepted : 2023.10.31
  • Published : 2023.12.31

Abstract

During crop disease surveys in 2019 and 2021, we observed gray mold symptoms on the leaves of wasabi (Eutrema japonicum) plants grown in vinyl greenhouses in Taebaek and Pyeongchang, Gangwon Province, Korea. The symptoms appeared at the edges of the leaves, where the infected areas turned black and rotted, and many gray molds formed on the lesions. The incidence of gray mold on the leaves in the vinyl greenhouses at the two locations ranged from 1 to 30%. Four fungal isolates were obtained from leaf lesions and identified as Botrytis cinerea based on morphological characteristics and phylogenetic analysis. Two isolates of B. cinerea were used for pathogenicity tests on the leaves of wasabi plants by artificial inoculation. The pathogenicity of the isolates was confirmed in leaves. The symptoms induced by the isolates were similar to those observed in wasabi leaves in the vinyl greenhouses investigated. This is the first report of B. cinerea causing gray mold in wasabi in Korea.

Keywords

Acknowledgement

This study was supported by a research grant (PJ014507012021) from the Rural Development Administration, Korea.

References

  1. Plants of the World Online. Eutrema japonicum (Miq.) Koidz. [Internet]. Kew: Royal Botanic Garden; 2023 [cited 2023 August 16]. Available from https://powo.science. kew.org/.
  2. Ellis MB. Dematiaceous Hyphomycetes. p. 178-9, Kew, Surrey, England: Commonwealth Mycological Institute; 1971.
  3. Dong L, Liu S, Li J, Tharreau D, Liu P, Tao D, Yang Q. A rapid and simple method for DNA preparation of Magnaporthe oryzae from single rice blast lesions for PCR-based molecular analysis. Plant Pathol J 2022;38:679-84. https://doi.org/10.5423/PPJ.NT.02.2022.0017
  4. Staats M, van Baarlen P, van Kan JA. Molecular phylogeny of the plant pathogenic genus Botrytis and the evolution of host specificity. Mol Biol Evol 2005;22:333-46. https://doi.org/10.1093/molbev/msi020
  5. Edgar RC. MUSCLE: multiple sequence alignment with high accuracy and high throughput. Nucleic Acids Res 2004;32:1792-7. https://doi.org/10.1093/nar/gkh340
  6. Van der Vlugt-Bergmans CJB, Brandwagt BF, Van't Klooster JW, Wagemakers CAM, van Kan JAL. 1993. Genetic variation and segregation of DNA polymorphisms in Botrytis cinerea. Mycol Res 1993;97:1193-200. https://doi.org/10.1016/S0953-7562(09)81284-7
  7. Kumar S, Stecher G, Tamura K. MEGA7: molecular evolutionary genetics analysis version 7.0 for bigger datasets. Mol Biol Evol 2016;33:1870-4. https://doi.org/10.1093/molbev/msw054
  8. The Phytopathological Society of Japan. Common names of plant diseases in Japan. p. 280, Tokyo, Japan: The Phytopathological Society of Japan;
  9. Macdonald JL, Punja ZK. 2016. Occurrence of botrytis leaf blight, anthracnose leaf spot, and white blister rust on Wasabia japonica in British Columbia. Can J Plant Pathol 2016;39:60-71 https://doi.org/10.1080/07060661.2017.1304021
  10. List of Plant Diseases in Korea. [Internet]. Seoul: Korean Society of Plant Pathology; 2023. [cited 2023 August 16]. Available from http://genebank.rda.go.kr/kplant disease.do.