International Journal of Industrial Entomology and Biomaterials

  • 제47권2호
  • /
  • Pages.147-154
  • /
  • 2023
  • /
  • 3022-7542(pISSN)
  • /
  • 2586-4785(eISSN)
한국잠사학회 (Korean Society of Sericultural Science)
권호 표지
DOI QR코드

DOI QR Code

A comparative analysis of characteristics and antioxidant capacity of Korean mulberries for efficient seedling cultivation

  • Chan Young Jeong (Department of Agricultural Biology, National Institute of Agricultural Sciences, Rural Development Administration) ;
  • Heon Woong Kim (Department of Agro-food Resources, National Institute of Agricultural Sciences, Rural Development Administration) ;
  • Seong Ryul Kim (Department of Agricultural Biology, National Institute of Agricultural Sciences, Rural Development Administration) ;
  • Hyun-Bok Kim (Department of Agricultural Biology, National Institute of Agricultural Sciences, Rural Development Administration) ;
  • Kee-Young Kim (Department of Agricultural Biology, National Institute of Agricultural Sciences, Rural Development Administration) ;
  • Seong-Wan Kim (Department of Agricultural Biology, National Institute of Agricultural Sciences, Rural Development Administration) ;
  • Jong Woo Park (Department of Agricultural Biology, National Institute of Agricultural Sciences, Rural Development Administration) ;
  • Ik Seob Cha (Department of Agricultural Biology, National Institute of Agricultural Sciences, Rural Development Administration) ;
  • Sang Kug Kang (Department of Agricultural Biology, National Institute of Agricultural Sciences, Rural Development Administration) ;
  • Ji Hae Lee (Department of Agricultural Biology, National Institute of Agricultural Sciences, Rural Development Administration)
  • 투고 : 2023.11.07
  • 심사 : 2023.12.19
  • 발행 : 2023.12.31
PDF 다운로드
⟨ 이전 논문 다음 논문 ⟩

초록

Mulberry exhibits unique characteristics and functionalities across various components, including the roots, branches, leaves, and fruits. However, despite numerous studies on mulberry, research on this plant at the seedling stage is insufficient. Therefore, this study aimed to compare the suitability for seedling cultivation and antioxidant effects of four Korean mulberry cultivars, namely, Daesim, Suhong, Simgang, and Cheongsu. In terms of seed weight, germination rate, and growth rate, Daesim was the most suitable cultivar for seedling production. Polyphenol and flavonoid content analysis showed that all cultivars, except for Cheongsu, showed the highest phenolic content at the 2-week seedling stage. Similarly, antioxidant assays using 2,2'-azino-di-3-ethylbenzthiazoline sulfonic acid (ABTS) and 2, 2-diphenyl-1-picrylhydrazyl (DPPH) radicals revealed that the antioxidant effect of all cultivars, except for Cheongsu, increased with cultivation at 2- 4- and 6-week. However, mulberry seedlings had a slower reaction rate against DPPH radical removal than mulberry leaves. In addition, ABTS radical scavenging activity showed a different correlation with polyphenol content. This phenomenon may be due to the different polyphenol compositions between mulberry leaves and seedlings. The results of this study suggest that mulberry seedlings exhibit different bioactivities from mulberry leaves, and component analysis is required in further research.

키워드

과제정보

This research was supported by the 'Research Program for Agriculture Science and Technology Development' (Grant No. PJ01675701) and the National Institute of Agricultural Sciences, Rural Development Administration, Republic of Korea.

참고문헌

  1. Awasthi AK, Nagaraja GM, Naik GV, Kanginakudru S, Thangavelu K, Nagaraju J (2004) Genetic diversity and relationships in mulberry (genus Morus) as revealed by RAPD and ISSR marker assays. BMC Genet 5, 1. https://doi.org/10.1186/1471-2156-5-1
  2. Bondet V, Brand-Williams W, Berset C (1997) Kinetics and mechanisms of antioxidant activity using the DPPH. free radical method. LWT-Food Sci Tech 3, 609-615. https://doi.org/10.1006/fstl.1997.0240
  3. Cao YG, Zheng XK, Yang FF, Li F, Qi M, Zhang YL, et al. (2018) Two new phenolic constituents from the root bark of Morus alba L. and their cardioprotective activity. Nat Prod Res 32, 391-398. https://doi.org/10.1080/14786419.2017.1309535
  4. Chen M, Huang W, Wang C, Nie H, Li G, Sun T, et al. (2014) High-mobility group box 1 exacerbates CCI(4)-induced acute liver injury in mice. Clinic Immunol 153, 56-63. https://doi.org/10.1016/j.clim.2014.03.021
  5. Chen YC, Tien Yj, Chen CH, Beltran FN, Amor EC, Wang RJ, et al. (2013) Morus alba and active compound oxyresveratrol exert anti-inflammatory activity via inhibition of leukocyte migration involving MEK/ERK signaling. BMC Complement Altern Med 13, 45. https://doi.org/10.1186/1472-6882-13-45
  6. Choi DW, Cho SW, Lee SG, Choi CY (2020) The beneficial effects of morusin, and isoprene flavonoid isolated from the root bark of Morus. Int J Mol Sci 21, 6541. https://doi.org/10.3390/ijms21186541
  7. Eo HJ, Park JH, Park GH, Lee MH, Lee JR, Koo JS, et al. (2014) Anti-inflammatory and anti-cancer activity of mulberry (Morus alba L.) root bark. BMC Complement Altern Med 14, 200. https://doi.org/10.1186/1472-6882-14-200
  8. Fadda A, Serra M, Molinu MG, Azara E, Barberis A, Sanna D (2014) Reaction time and DPPH concentration influence antioxidant activity and kinetic parameters of bioactive molecules and plant extracts in the reaction with the DPPH radical. J Food Compos Anal 35, 112-119. https://doi.org/10.1016/j.jfca.2014.06.006
  9. Floegel A, Kim DO, Chung SJ, Koo SI, Chun OK (2011) Comparison of ABTS/DPPH assays to measure antioxidant capacity in popular antioxidant-rich US foods. J Food Compos Aanal 24, 1043-1048. https://doi.org/10.1016/j.jfca.2011.01.008
  10. Gryn-Rynko A, Bazylak G, Olszewska-Slonina D (2016) New potential phytotherapeutics obtained from white mulberry (Morus alba L.) leaves. Biomed Pharmacother 84, 628-636. https://doi.org/10.1016/j.biopha.2016.09.081
  11. Jung SH, Han JH, Park HS, Lee DH, Kim SJ, Cho HS, et al. (2019) Effects of unaltered and bioconverted mulberry leaf extracts on cellular glucose uptake and antidiabetic action in animals. BMC Complement Altern Med 19, 19-55. https://doi.org/10.1186/s12906-019-2460-5
  12. Katsube T, Imawaka N, Kawano Y, Yamazaki Y, Shiwaku K, Yamane Y (2006) Antioxidant flavonol glycosides in mulberry (Morus alba L.) leaves isolated based on LDL antioxidant activity. Food Chem 97, 25-31. https://doi.org/10.1016/j.foodchem.2005.03.019
  13. Kim GN, Jang HD (2011) Flavonol content in the water extract of the mulberry (Morus alba L.) leaf and their antioxidant capacities. J Food Sci 76, 869-873. https://doi.org/10.1111/j.1750-3841.2011.02262.x
  14. Kim HB (2005) Anti-oxidative capacity analysis of water-soluble substances according to varieties and maturity stages in mulberry leaves and fruits. J Seric Entomol Sci 47, 62-67.
  15. Kim TI, Kwon EB, Oh YC, Go Y, Choi JG (2021) Mori ramulus and its major component Morusin inhibit herpes simplex virus type 1 replication and the virus-induced reactive oxygen species. Am J Chin Med 49, 163-179. https://doi.org/10.1142/S0192415X21500099
  16. Lee S, Koo B, Ju WT, Kim HB, Kweon HY, Lee JH (2021) Effect of extraction conditions on chemical composition and antioxidant properties of mulberry fruit. Int J Indust Entomol 42, 1-8. http://dx.doi.org/10.7852/ijie.2021.42.2.1
  17. Li HX, Jo E, Myung CS, Kim YH, Yang SY (2018) Lipolytic effect of compounds isolated from leaves of mulberry (Morus alba L.) in 3T3-L1 adipocytes. Nat Prod Res 32, 1963-1966. https://doi.org/10.1080/14786419.2017.1354190
  18. Li R, Su X, Zhou R, Zhang Y, Wang T (2022) Molecular mechanism of mulberry response to drought stress revealed by complementary transcriptomic and iTRAQ analyses. BMC Plant Biol 22, 36. https://doi.org/10.1186/s12870-021-03410-x
  19. Lim DW, Jung JW, Park JH, Baek NI, Kim YT, Kim IH, et al. (2015) Antidepressant-like effects of sanggenon G, isolated from the root bark of Morus alba, in rats: involvement of the serotonergic system. Biol Pharm Bull 38, 1772-1778. https://doi.org/10.1248/bpb.b15-00471
  20. Lin Z, Gan T, Huang Y, Bao L, Liu S, Cui X, et al. (2022) Anti-inflammatory activity of mulberry leaf flavonoids in vitro and in vivo. Int J Mol Sci 23, 7694. https://doi.org/10.3390/ijms23147694
  21. Maji SK, Roy S, Basu A, Pandey KC, Samanta S, Vashisht K, et al. (2023) Geo-environmental factors and the effectiveness of mulberry leaf extract in managing malaria. Sci Rep 13, 14808. https://doi.org/10.1038/s41598-023-41668-3
  22. Novo E, Parola M (2008) Redox mechanisms in hepatic chronic wound healing and fibrogenesis. Fibrogen Tissue Repair 1, 5. https://doi.org/10.1186/1755-1536-1-5
  23. Park SS, Sung SH, Ryu YB, Cho YU, Choi YJ, Park KH, et al. (2008) Growth inhibition of Propionibacterium acnes by mycelial culture broth of Paecilomyces japonica in the mulberry leaf extract. J Mushroom 6, 32-37.
  24. Qiao J, Li H, Chen J, Shi Y, Li N, Zhu P, et al. (2023) Mulberry fruit repairs alcoholic liver injury by modulating lipid metabolism and the expression of miR-155 and PPARα in rats. Funct Integr Genomics 23, 261. https://doi.org/10.1007/s10142-023-01131-y
  25. Qu L, Liang X, Tian G, Zhang G, Wu Q, Huang X, et al. (2021) Efficacy and safety of mulberry twig alkaloids tablet for the treatment of type 2 diabetes: A multicenter, randomized, double-blind, double-dummy, and parallel controlled clinical trial. Diabetes Care 44, 1324-1333. https://doi.org/10.2337/dc20-2109
  26. Rahul K, Kweon HY, Kim HB, Lee JH, Makwana P (2022) Enhancement of polyphenols, flavonoids and antioxidant activities in water extract of mulberry (Morus alba L.) root bark by steam treatment. Int J Indust Entomol 44, 21-27. http://dx.doi.org/10.7852/ijie.2022.44.1.21
  27. Ren X, Sun Y, Guo Q, Liu H, Jiang H, He X, et al. (2022) Ameliorating effect of the total flavonoids of Morus nigra L. on prediabetic mice based on regulation of inflammation and insulin sensitization. J Agric Food Chem 70, 12484-12501. https://doi.org/10.1021/acs.jafc.2c04970
  28. Sakai A, Otsuka K (1967) Survival of plant tissue at super-low temperatures V. And electron microscope study of ice in cortical cells cooled rapidly. Plant Physiol 42, 1680-1694. https://doi.org/10.1104/pp.42.12.1680
  29. Shi B, Qian J, Miao H, Zhang S, Hu Y, Liu P, et al. (2023) Mulberroside A ameliorates CCI4-induced liver fibrosis in mice via inhibiting pro-inflammatory response. Food Sci Nutr 11, 3433-3441. https://doi.org/10.1002/fsn3.3333
  30. Sood P, Singh V, Shri R (2023) Morus alba fruit diet ameliorates cognitive deficit in mouse model of streptozotocin-induced memory impairment. Metab Brain Dis 38, 1657-1669. https://doi.org/10.1007/s11011-023-01199-2
  31. Sood P, Singh V, Shri R (2023) Morus alba fruit extract and its fractions ameliorate streptozotocin induced cognitive deficit in mice via modulating oxidative and cholinergic systems. Neurochem Res. doi:10.1007/s11064-023-040090-4.
  32. Tomczyk M, Milek M, Sidor E, Kapusta I, Litwinczuk W, Puchalski C, et al. (2019) The effect of adding the leaves and fruits of Morus alba to rape honey on its antioxidant properties, polyphenolic profile, and amylase activity. Molecules 25, 84. https://doi.org/10.3390/molecules25010084
  33. Wu YX, Kim YJ, Kwon TH, Tan CP, Son KH, Kim T (2020) Anti-inflammatory effects of mulberry (Morus alba L.) root bark and its active compounds. Nat Prod Res 34, 1786-1790. https://doi.org/10.1080/14786419.2018.1527832
  34. Wu YX, Kim YJ, Li S, Yun MC, Yoon JM, Kim JY, et al. (2015) Anti-obese effects of mulberry (Morus alba L.) root bark through the inhibition of digestive enzymes and 3T3-L1 adipocyte differentiation. Korean J Food Preserv 22, 27-35. https://doi.org/10.11002/kjfp.2015.22.1.27
  35. Yamatake Y, Shibata M, Nagai M (1976) Pharmacological studies on root bark of mulberry tree (Morus alba L.). Jpn J Pharmacol 26, 461-469. https://doi.org/10.1254/jjp.26.461
  36. Yao Y, Chen Y, Chen H, Pan X, Li X, Liu W, et al. (2024) Black mulberry extract inhibits hepatic adipogenesis through AMPK/mTOR signaling pathway in T2DM mice. J Ethnopharmacol 319, 117216. https://doi.org/10.1016/j.jep.2023.117216
  37. Zheng XK, Cao YG, Ke YY, Zhang YL, Li F, Gong JH, et al. (2017) Phenolic constituents from the root bark of Morus alba L. and their cardioprotective activity in vitro. Phytochem 135, 128-134. https://doi.org/10.1016/j.phytochem.2016.12.006