International Journal of Industrial Entomology and Biomaterials

  • 제47권2호
  • /
  • Pages.126-133
  • /
  • 2023
  • /
  • 3022-7542(pISSN)
  • /
  • 2586-4785(eISSN)
한국잠사학회 (Korean Society of Sericultural Science)
권호 표지
DOI QR코드

DOI QR Code

Cryopreservation of winter-dormant mulberry buds using two-step freezing

  • Hyeok Gyu Kwon (Industrial Insect and Sericulture Division, National Institute of Agricultural Science, RDA) ;
  • Kee Young Kim (Industrial Insect and Sericulture Division, National Institute of Agricultural Science, RDA) ;
  • Seul Ki Park (Industrial Insect and Sericulture Division, National Institute of Agricultural Science, RDA) ;
  • Chan Young Jeong (Industrial Insect and Sericulture Division, National Institute of Agricultural Science, RDA) ;
  • Sang-Kug Kang (Industrial Insect and Sericulture Division, National Institute of Agricultural Science, RDA) ;
  • Ik-Seob Cha (Industrial Insect and Sericulture Division, National Institute of Agricultural Science, RDA) ;
  • Seong-Wan Kim (Industrial Insect and Sericulture Division, National Institute of Agricultural Science, RDA) ;
  • Seong-Ryul Kim (Industrial Insect and Sericulture Division, National Institute of Agricultural Science, RDA) ;
  • Hyo-Eun Lee (Department of Agricultural Life Science, Sunchon National University) ;
  • Haeng-Hoon Kim (Department of Agricultural Life Science, Sunchon National University) ;
  • Jong Woo Park (Industrial Insect and Sericulture Division, National Institute of Agricultural Science, RDA)
  • 투고 : 2023.10.18
  • 심사 : 2023.11.28
  • 발행 : 2023.12.31
PDF 다운로드
⟨ 이전 논문 다음 논문 ⟩

초록

Genetic resources of mulberry trees are commonly preserved as trophosomes, which are vulnerable to environmental factors, such as natural disasters, diseases, and pests. This study establishes a basic protocol for ultra-low temperature cryopreservation of mulberry trees using a two-step freezing process. The procedure was established using the "Daeshim" variety and then tested on genetic resources from 24 other mulberry varieties. Samples were first dried to a moisture content of 33-43% in a low-temperature forced-air chamber at -5 ℃, then slowly frozen from -5 ℃ to -20 ℃, and preserved in liquid nitrogen (-196 ℃). To determine the regeneration rate, isolated dormant buds were inoculated into MS basal medium, and grown shoots were grafted onto 1-year-old rootstock via chip budding and then cultured. After freezing in liquid nitrogen, the "Daeshim" variety exhibited a survival and regeneration rate of more than 70% and 50%, respectively. Applying the two-step freezing process to genetic resources from 24 mulberry species yielded average survival and regeneration rates of 85.3% and 75.5%, respectively. Morus alba showed survival and regeneration rates of 100%, confirming the efficacy of the two-step freezing method. These results indicate the high feasibility of ultra-low-temperature cryopreservation through two-step freezing of dormant buds from mulberry genetic resources. Additional research is required into the variations in regeneration rates with freezing period in liquid nitrogen.

키워드

과제정보

This study was supported by the 2023 RDA fellowship program of National Institute of Agricultural Science and was supported by a grant (No. PJ01558101) from the Rural Development Administration, Republic of Korea.

참고문헌

  1. Ahn YH, Skai A (1994) Survival by vitrification of Dendranthema graniflorum L.shoot tips for cryopreservtion. J Korean Soc Hort Sci 35, 499-506.
  2. Ananda RA, Rekha C, Malik SK, Suseel K, Radhakrishnan R, Qadri SMH (2009) Mulberry Biodiversity Conservation through Cryopreservation. In Vitro Cell Dev Bio Plant 45, 639-649. http://dx.doi.org/10.1007/s11627-008-9185-3
  3. Benson EE, Withers LA (1998) The application of germplasm storage in biotechnology. Plant cell biotechnology, NATO ASI Series (Series H: Cell Biology) vol. 18, Springer, Berlin. pp. 431-444. https://doi.org/10.1007/978-3-642-73157-0_43
  4. Engelmann F (2004) Plant cryopreservation: Progress and prospects. In Vitro Cell Devl Biol Plant 40, 427-433. https://www.jstor.org/stable/4293769 https://doi.org/10.1079/IVP2004541
  5. Fatima S, Mujib A, Nasim SA, Siddiqui ZH (2009) Cryopreservation of embryogenic cell suspensions of Catharanthus roseus L. (G) Don. Plant Cell Tiss Organ Cult, 98, 1-9. http://dx.doi.org/10.1007/s11240-009-9532-z
  6. Forsline PL, Towill LE, Waddell JW, Stushnoff C, Lamboy WF, McFerson JR (1998) Recovery and longevity of cryopreserved apple buds. J Amer Soc Hort Sci 123, 365-370. https://doi.org/10.21273/JASHS.123.3.365
  7. Fukai S (1990) Cryopreservation of chrysanthemum shoot tips. Sci Hortic 45, 167-174. https://doi.org/10.1016/0304-4238(90)90079-T
  8. Halmagyi A, Fischer K, Mix W, Schumacher HM (2004) Cryopreservation of Chrysanthemum morifolium (Dendranthema Grandiflora Ramat.) using different approaches. Plant Cell Rep 22, 371-375. https://doi.org/10.1007/s00299-003-0703-9
  9. Hitmi A, Barthomeuf C, Sallanon H (1999) Cryopreservation of Chrysanthemum cinerariaefolium shoot tips. Effects of pretreatment conditions and retention of biosynthetic capacity. CryoLetters 20, 109-120. https://doi.org/10.1016/S0176-1617(00)80081-4
  10. Jeong CY, Kang SK, Kim NS, Cha IS, Kim SW, Park JW, et al. (2022) Microsatellite analysis of 20 mulberry varieties preserved in Korea. Int J Indust Entomol 45, 49-55. DOI: http://dx.doi.org/10.7852/ijie.2022.45.2.49
  11. Ju WT, Kwon OC, Kim YS, Kim HB, Sung GB, Kim JG (2018) Flavonoids analysis about mulberry fruit of Korean mulberry cultivar, ''Daeshim''. Int J Indust Entomol 37, 43-48, DOI: http://dx.doi.org/10.7852/ijie.2018.37.2.43
  12. Kim HB, Kweon HY, Ju WT, Jo YY, Kim YS (2021) Nutrient composition of Korean mulberry fruits (Morus sp.) dried with low temperature vacuum dryer using microwave. Int J Indust Entomol 42, 14-20. DOI: http://dx.doi.org/10.7852/ijie.2021.42.1.14
  13. Lambardi M (2012) From the field to the tank: the cryobanking of ancient Italian apple germplasm by the dormantbud technique. Cryobiology 65, 342 https://doi.org/10.1016/j.cryobiol.2012.07.013.
  14. Martin C, Gonzalez BE (2005). Survival and genetic of Dendranthema grandiflora Tzelev shoot apices after cryopreservation by vitrification and encapsulation-dehydration. Cryobiology 51, 281-289. https://doi.org/10.1016/j.cryobiol.2005.08.001
  15. Matsumoto T, Mochida K, Itamura H, Sakai A (2001) Cryopreservation of persimmon (Diospyros kaki Thunb.) by vitrification of dormant shoot tips. Plant Cell Rep 20, 398-402. https://doi.org/10.1007/ s002990100350.
  16. Rahul K, Kweon HY, Kim HB, Lee JH, Makwana P (2022) Enhancement of polyphenols, flavonoids and antioxidant activities in water extract of mulberry (Morus alba L.) root bark by steam treatment. Int J Indust Entomol 44, 21-27. DOI: http://dx.doi.org/10.7852/ijie.2022.44.1.27
  17. Sakai A (1960) Survival of the twig of woody plants at -196oC. Nature 185, 393-394. https://doi.org/10.1038/185393a0
  18. Sakai A, Kobayashi S, Oiyama I (1990) Cryopreservation of nuclear cells of navel orange (Citrus sinensis Osb. var. brasiliensis Tanaka) by vitrification. Plant Cell Rep 9, 30-33. https://doi.org/10.1007/BF00232130
  19. Sakai A, Matsumoto T, Hirai D, Niino T (2000) Newly developed encapsulation-dehydration protocol for plant cryopreservation. CryoLetters 21, 53-62.
  20. Sung GB, Nam HW, Lee SU, Park KJ (2002) Breeding of cold resistant mulberry cultivar "Hansungppong" (Morus alba L.). Korean J Seric Sci 44, 45-50.
  21. Towill LE, Bonnart R (2005) Cryopreservation of apple using non-desiccated sections from winter-collected scions. CryoLetters 26, 323-332.
  22. Towill LE, Forsline PL, Walters C, Waddell JW, Laufman J (2004) Cryopreservation of Malus germplasm using a winter vegetative bud method: Results from 1915 accessions. CryoLetters 25, 323-334.
  23. Yi JY, Lee GA, Lee SY, Chung JW, Shin IS (2013) Cryopreservation of winter-dormant apple buds using two-step freezing. Plant Breed. Biotech 1, 283-289. http://dx.doi.org/10.9787/PBB.2013.1.3.283