한국해양바이오학회지 (Journal of Marine Bioscience and Biotechnology)

한국해양바이오학회 (The Korean Society for Marine Biotechnology)
권호 표지
DOI QR코드

DOI QR Code

방사무늬 김 열수추출물의 RAW 264.7 세포에서의 면역 증진 효과

Immune Enhancing Effects of Pyropia yezoensis Hydrothermal Extract in RAW 264.7 Cells

  • 장고은 (해양헬스케어 유효성실증센터) ;
  • 박보람 (경운대학교 보건복지대학 치위생학과) ;
  • 이슬아 (해양헬스케어 유효성실증센터) ;
  • 김춘성 (해양헬스케어 유효성실증센터)
  • Goeun Jang (Marine Healthcare Research & Evaluation Center, Chosun University) ;
  • Bo-Ram Park (Department of Dental Hygiene, College of Health and Welfare, Kyungwoon University) ;
  • Seul Ah Lee (Marine Healthcare Research & Evaluation Center, Chosun University) ;
  • Chun Sung Kim (Marine Healthcare Research & Evaluation Center, Chosun University)
  • 투고 : 2023.06.07
  • 심사 : 2023.07.29
  • 발행 : 2023.12.31
PDF 다운로드
⟨ 이전 논문 다음 논문 ⟩

초록

This study aimed to investigate the immunomodulatory function of Pyropia yezoensis hydrothermal (water) extract (PYWE) in comparison to the group treated only with lipopolysaccharides (LPS) in RAW264.7 cells. LPS is known to be an inflammatory mediator that activates macrophages, leading to the secretion of nitric oxide (NO), inducible nitric oxide synthase (iNOS), tumor necrosis factor-α (TNF-α), and interleukin-6 (IL-6) as defense responses. Through enzyme-linked immunoassay and western blot analyses, it was observed that PYWE increased the expression levels of NO, iNOS, TNF-α, and IL-6 in RAW264.7 cells in a dose-dependent manner, although to a lesser extent compared with the group treated with LPS alone. In addition, the study examined the mitogen-activated protein kinases (MAPKs) pathway, which regulates various cellular activities, including gene expression, mitosis, cell differentiation, transformation, survival, and death. The western blot analysis confirmed that PYWE also regulated the MAPKs pathway. Furthermore, the expression levels of immunomodulatory-related factors increased in the group treated with PYWE compared with the control group. Even though the effects of PYWE were usually less strong than those of LPS, the effects of PYWE increased with increasing doses compared to the control group. This suggests that PYWE could be used to control the immune system.

키워드

과제정보

이 논문은 2021년도 해양수산부 재원으로 해양수산과학기술진흥원의 지원을 받아 수행된 연구입니다(20210656, 빅데이터 기반 해양바이러스 제어 및 마린바이오틱스 개발사업).

참고문헌

  1. Chaplin DD. Overview of the immune response. J Allergy Clin Immunol. 2010 Feb;125(2 Suppl 2):S3-23. doi: 10.1016/j.jaci.2009.12.980. PMID: 20176265; PMCID: PMC2923430. 
  2. Medzhitov R, Janeway CA Jr. Innate immunity: impact on the adaptive immune response. Curr Opin Immunol. 1997 Feb;9(1):4-9. doi: 10.1016/s0952-7915(97)80152-5. PMID: 9039775. 
  3. Alexander C, Rietschel ET. Bacterial lipopolysaccharides and innate immunity. J Endotoxin Res. 2001;7(3):167-202. PMID: 11581570.  https://doi.org/10.1179/096805101101532675
  4. Sun L, Wang X, Saredy J, Yuan Z, Yang X, Wang H. Innate-adaptive immunity interplay and redox regulation in immune response. Redox Biol. 2020 Oct;37:101759. doi: 10.1016/j.redox.2020.101759. Epub 2020 Oct 10. PMID: 33086106; PMCID: PMC7575795. 
  5. Baeuerle, Patrick A., and Thomas Henkel. "Function and activation of NF-kappaB in the immune system." Annual review of immunology 12.1 (1994): 141-179.  https://doi.org/10.1146/annurev.iy.12.040194.001041
  6. Hwang, Kyung-A., Yu-Jin Hwang, and Jin Song. "Aster yomena extract ameliorates pro-inflammatory immune response by suppressing NF-κB activation in RAW 264.7 cells." Journal of the Chinese Medical Association 81.2 (2018): 102-110.  https://doi.org/10.1016/j.jcma.2017.06.017
  7. Liu, Yusen, Edward G. Shepherd, and Leif D. Nelin. "MAPK phosphatases-regulating the immune response." Nature Reviews Immunology 7.3 (2007): 202-212.  https://doi.org/10.1038/nri2035
  8. Reddy, D. Bharat, and Pallu Reddanna. "Chebulagic acid (CA) attenuates LPS-induced inflammation by suppressing NF-κB and MAPK activation in RAW 264.7 macrophages." Biochemical and Biophysical Research Communications 381.1 (2009): 112-117.  https://doi.org/10.1016/j.bbrc.2009.02.022
  9. Koh, Timothy J., and Luisa Ann DiPietro. "Inflammation and wound healing: the role of the macrophage." Expert reviews in molecular medicine 13 (2011): e23. 
  10. Jones, Jonathan DG, and Jeffery L. Dangl. "The plant immune system." nature 444.7117 (2006): 323-329.  https://doi.org/10.1038/nature05286
  11. Han, Guan-Zhu. "Origin and evolution of the plant immune system." New Phytologist 222.1 (2019): 70-83.  https://doi.org/10.1111/nph.15596
  12. Trinh, Tuy An, et al. "Effect of herbal formulation on immune response enhancement in RAW 264.7 macrophages." Biomolecules 10.3 (2020): 424. 
  13. Magrone, Thea, et al. "Olive leaf extracts act as modulators of the human immune response." Endocrine, Metabolic & Immune Disorders-Drug Targets (Formerly Current Drug Targets-Immune, Endocrine & Metabolic Disorders) 18.1 (2018): 85-93.  https://doi.org/10.2174/1871530317666171116110537
  14. Brown, Emma M., et al. "Seaweed and human health." Nutrition reviews 72.3 (2014): 205-216.  https://doi.org/10.1111/nure.12091
  15. Abera T, Admassu H, Abraha B, Yang R, Zhao W. Proximate, mineral and amino acid composition of dried laver (Porphyra spp.). J Acad Ind Res 2018; 6(9): 149-154. 
  16. Kim KW, Hwang JH, Oh MJ, Kim MY, Choi MR, Park WM. Studies on the major nutritional components of commercial dried lavers (Porphyra yezoensis) cultivated in Korea. Korean J Food Preserv 2014; 21(5): 702-709.  https://doi.org/10.11002/kjfp.2014.21.5.702
  17. Qu W, Ma H, Pan Z, Luo L, Wang Z, He R. Preparation and antihypertensive activity of peptides from Porphyra yezoensis. Food Chem 2010; 123(1): 14-20.  https://doi.org/10.1016/j.foodchem.2010.03.091
  18. Shin ES, Hwang HJ, Kim IH, Nam TJ. A glycoprotein from Porphyra yezoensis produces anti-inflammatory effects in liposaccharide-stimulated macrophages via the TLR4 signaling pathway. Int J Mol Med 2011; 28(5): 809-815. 
  19. Isaka S, Cho K, Nakazono S, Abu R, Ueno M, Kim D, et al. Antioxidant and anti-inflammatory activities of porphyran isolated from discolored nori (Porphyra yezoensis). Int J Biol Macromol 2015; 74: 68-75.  https://doi.org/10.1016/j.ijbiomac.2014.11.043
  20. Eitsuka T, Nakagawa K, Igarashi M, Miyazawa T. Telomerase inhibition by sulfoquinovosyldiacylglycerol from edible purple laver (Porphyra yezoensis). Cancer Lett 2004; 212(1): 15-20.  https://doi.org/10.1016/j.canlet.2004.03.019
  21. Li L, Saga N, Mikami K. Effects of cell wall synthesis on cell polarity in the red alga Porphyra yezoensis. Plant Signal Behav 2008; 3(12): 1126-1128.  https://doi.org/10.4161/psb.3.12.7041
  22. Hwang HJ, Kwon MJ, Kim IH, Nam TJ. Chemoprotective effects of a protein from the red algae Porphyra yezoensis on acetaminophen induced liver injury in rats. Phytother Res 2008; 22: 1149-1153. https://doi.org/10.1002/ptr.2368