Korean Journal of Plant Resources (한국자원식물학회지)

The Plant Resources Society of Korea (한국자원식물학회)
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Hibiscus manihot leaves Attenuate Accumulation of Lipid Droplets by Activating Lipolysis, Browning and Autophagy, and Inhibiting Proliferation of 3T3-L1 Cells

  • Na Gyeong Geum (Department of Forest Science, Andong National University) ;
  • Jeong Won Choi (Department of Forest Science, Andong National University) ;
  • Hyeok Jin Choi (Department of Forest Science, Andong National University) ;
  • Gwang Hyeon Ryu (Department of Forest Science, Andong National University) ;
  • Jin Boo Jeong (Department of Forest Science, Andong National University)
  • Received : 2023.10.16
  • Accepted : 2023.10.30
  • Published : 2023.12.01
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Abstract

In the present study, the effects of HML on lipolysis, adipocyte browning, autophagy, and proliferation were investigated. HML affected lipolysis by increasing the protein levels of ATGL and HSL, and phosphorylation levels of HSL and AMPK. Furthermore, HSL decreased the perilipin-1 levels. In addition, free glycerol content was increased by HML treatment. HML affected adipocyte browning by increasing the protein levels of UCP-1, PGC-1α, and PRDM16. In addition, HML affected autophagy by increasing the levels of LC3-I and LC3-II, and decreasing those of SQSTM1/p62. Moreover, HML affected adipocyte proliferation by suppressing the proliferation of 3T3-L1 cells due to arrest of the cell cycle via blocking the expression of β-catenin and cyclin D1. These results suggest that HML induces lipolysis, adipocyte browning, autophagy, and inhibits excessive proliferation of adipocytes.

Keywords

Acknowledgement

This work was supported by the R&D Program for Forest Science Technology (Project No. 2021377C10-2123-BD02) provided by the Korea Forest Service (Korea Forestry Promotion Institute) and the Basic Science Research Program through the National Research Foundation of Korea (NRF) funded by the Ministry of Education (NRF-2018R1A6A1A03024862).

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