Korean Journal of Plant Resources (한국자원식물학회지)

The Plant Resources Society of Korea (한국자원식물학회)
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Regeneration and Acclimatization of Regenerants in Long-term in vitro Culture of Japanese Blood Grass (Imperata cylindrica 'Rubra')

  • Eon-Yak Kim (Department of Plant Production Sciences, Graduate School of Sunchon National University) ;
  • In-Jin Kang (Department of Plant Production Sciences, Graduate School of Sunchon National University) ;
  • Ye-Jin Lee (Department of Plant Production Sciences, Graduate School of Sunchon National University) ;
  • Baul Yang (Department of Plant Production Sciences, Graduate School of Sunchon National University) ;
  • Vipada Kantayos (Department of Botany, Faculty of Liberal Arts and Science, Kasetsart University Kamphaeng Saen Campus) ;
  • Chang-Hyu Bae (Department of Plant Production Sciences, Graduate School of Sunchon National University)
  • Received : 2023.11.01
  • Accepted : 2023.11.23
  • Published : 2023.12.01
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Abstract

Long-term culture of cell lines is an important issue in in vitro culture and in plant science. In this study, the regeneration ability and ex vitro acclimatization of regenerants were evaluated. The ploidy level of regenerants derived from long-term cultured cell lines was measured in Imperata cylindrica 'Rubra', Poaceae. Adventitious buds (shoots) were successfully induced from five-year-cultured calli on MS medium containing 0.1 mg/L BA or 2.0 mg/L TDZ, combined with 0.01 mg/L auxins (IAA, IBA, NAA and 2,4-D), respectively. Adventitious roots were also induced on MS medium containing 0.01 mg/L auxins (IBA, NAA and 2,4-D), respectively. Interestingly, regenerants with both red and green leaf were successfully obtained when regenerants were cultured on MS medium with 9% sucrose. Regenerants derived from long-term cultured calli were transferred to pots using an optimal acclimatization process and successfully adapted to both pot and soil conditions. Moreover, the ploidy level was measured using calli and regenerants that had been kept on MS medium containing various kinds of plant growth regulators (PGRs).

Keywords

Acknowledgement

This research was supported by the MSIT (Ministy of Science and ICT), Korea, under the Grand Information Technology Research Center support program (IITP-2023-2020-0-01489) supervised by the IITP (Institute for Information & communications Technology Planning & Evaluation).

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