Current Optics and Photonics

Optical Society of Korea (한국광학회)
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Super-resolution Microscopy with Adaptive Optics for Volumetric Imaging

  • Park, Sangjun (Department of Medical Life Sciences, College of Medicine, The Catholic University of Korea) ;
  • Min, Cheol Hong (Department of Medical Life Sciences, College of Medicine, The Catholic University of Korea) ;
  • Han, Seokyoung (Department of Mechanical Engineering, University of Louisville) ;
  • Choi, Eunjin (Department of Medical Life Sciences, College of Medicine, The Catholic University of Korea) ;
  • Cho, Kyung-Ok (Department of Biomedicine & Health Sciences, College of Medicine, The Catholic University of Korea) ;
  • Jang, Hyun-Jong (Department of Biomedicine & Health Sciences, College of Medicine, The Catholic University of Korea) ;
  • Kim, Moonseok (Department of Medical Life Sciences, College of Medicine, The Catholic University of Korea)
  • Received : 2022.10.16
  • Accepted : 2022.11.07
  • Published : 2022.12.25
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Abstract

Optical microscopy is a useful tool for study in the biological sciences. With an optical microscope, we can observe the micro world of life such as tissues, cells, and proteins. A fluorescent dye or a fluorescent protein provides an opportunity to mark a specific target in the crowd of biological samples, so that an image of a specific target can be observed by an optical microscope. The optical microscope, however, is constrained in resolution due to diffraction limit. Super-resolution microscopy made a breakthrough with this diffraction limit. Using a super-resolution microscope, many biomolecules are observed beyond the diffraction limit in cells. In the case of volumetric imaging, the super-resolution techniques are only applied to a limited area due to long imaging time, multiple scattering of photons, and sample-induced aberration in deep tissue. In this article, we review recent advances in super-resolution microscopy for volumetric imaging. The super-resolution techniques have been integrated with various modalities, such as a line-scan confocal microscope, a spinning disk confocal microscope, a light sheet microscope, and point spread function engineering. Super-resolution microscopy combined with adaptive optics by compensating for wave distortions is a promising method for deep tissue imaging and biomedical applications.

Keywords

Acknowledgement

FIG. 1 and FIG. 4 were created with BioRender.com.

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