The Korea Journal of Herbology (대한본초학회지)

The Korea Association of Herbology (대한본초학회)
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Anti-inflammatory Activity of Cynanchi Atrati Radix Et Rhizoma Water Extracts via Regulation of MAPK in LPS-induced Murine Macrophage Cell Line, RAW 264.7

LPS로 유도된 마우스 대식세포주인 RAW264.7에서 MAPK 조절에 의한 백미 물추출물의 항염증 활성

  • Lee, Sang-Ho (Nutrex Technology Co., Ltd.) ;
  • Yoo, Ji-Hyun (Department of Herbal Health & Pharmacy, Joongbu University) ;
  • Kil, Ki-Jung (Department of Herbal Health & Pharmacy, Joongbu University)
  • 이상호 ((주)뉴트렉스테크놀러지) ;
  • 유지현 (중부대학교 한방보건제약학과) ;
  • 길기정 (중부대학교 한방보건제약학과)
  • Received : 2022.10.12
  • Accepted : 2022.11.25
  • Published : 2022.11.30
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Abstract

Objectives : To develop natural ingredients that help prevent or treat anti-inflammatory-related diseases and use themas basic data, we investigated anti-inflammatory activity of Cynanchi Atrati Radix Et Rhizoma water extracts(CWE) in lipopolysaccharide(LPS)-induced murine macrophage cell line, RAW 264.7 cells. Methods : The cell viabilities were evaluated with RAW 264.7 cells. The production of nitric oxide(NO), prostaglandin E2(PGE2), pro-inflammatory cytokines such tumor necrotic factor(TNF)-α and interleukin(IL)-6 were assessed in LPS-induced RAW 264.7 cell treated with CWE. Furthermore, the protein expression of inducible nitric oxide synthase (iNOS), cyclooxygenase-2(COX-2), and mitogen-activated protein kinase(MAPK) were assessed by western blotting. Results : In RAW 264.7 cell, the cell viability by CWE treatment was more than 98.4% at a concentration of 100-400 ㎍/mL. At a concentration of 800 ug/ml of CWE, the cell viability was as low as 86%. At doses of 100, 200 and 400 ㎍/mL, CWE inhibited the production of NO, PGE2, TNF-𝛼 and IL-6 in a dose-dependent manner and also decreased the expression of iNOS and COX-2 from LPS-induced RAW 264.7 cells. In addition, CWE significantly inhibited the MAPK pathway including decreased the phosphorylation of the p38, c-Jun N-terminal kinase(JNK) and extracellular signal-regulated kinase(ERK1/2). Conclusions : Our study provides evidence that CWE inhibits the production of main pro-inflammatory molecules in LPS-induced RAW 264.7 cells via expression of p38, JNK, and ERK1/2 MAPK signaling pathways. Therefore, CWE is expected to be widely used as a natural ingredient for anti-inflammatory functional foods or pharmaceuticals in the future.

Keywords

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