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Synthesis of ginsenoside Rb1-imprinted magnetic polymer nanoparticles for the extraction and cellular delivery of therapeutic ginsenosides

  • Liu, Kai-Hsi (Department of Internal Medicine, Division of Cardiology, Zuoying Branch of Kaohsiung Armed Forces General Hospital) ;
  • Lin, Hung-Yin (Department of Chemical and Materials Engineering, National University of Kaohsiung) ;
  • Thomas, James L. (Department of Physics and Astronomy, University of New Mexico) ;
  • Shih, Yuan-Pin (Department of Chemical and Materials Engineering, National University of Kaohsiung) ;
  • Yang, Zhuan-Yi (Department of Chemical Engineering, I-Shou University) ;
  • Chen, Jen-Tsung (Department of Life Sciences, National University of Kaohsiung) ;
  • Lee, Mei-Hwa (Department of Materials Science and Engineering, I-Shou University)
  • Received : 2021.04.19
  • Accepted : 2022.01.26
  • Published : 2022.09.01

Abstract

Background: Panax ginseng (ginseng) is a traditional medicine that is reported to have cardioprotective effects; ginsenosides are the major bioactive compounds in the ginseng root. Methods: Magnetic molecularly imprinted polymer (MMIP) nanoparticles might be useful for both the extraction of the targeted (imprinted) molecules, and for the delivery of those molecules to cells. In this work, plant growth regulators were used to enhance the adventitious rooting of ginseng root callus; imprinted polymeric particles were synthesized for the extraction of ginsenoside Rb1 from root extracts, and then employed for subsequent particle-mediated delivery to cardiomyocytes to mitigate hypoxia/reoxygenation injury. Results: These synthesized composite nanoparticles were first characterized by their specific surface area, adsorption capacity, and magnetization, and then used for the extraction of ginsenoside Rb1 from a crude extract of ginseng roots. The ginsenoside-loaded MMIPs were then shown to have protective effects on mitochondrial membrane potential and cellular viability for H9c2 cells treated with CoCl2 to mimic hypoxia injury. The protective effect of the ginsenosides was assessed by staining with JC-1 dye to monitor the mitochondrial membrane potential. Conclusion: MMIPs can play a dual role in both the extraction and cellular delivery of therapeutic ginsenosides.

Keywords

Acknowledgement

The authors would like to appreciate the Ministry of Science and Technology of ROC under Contract nos.: MOST 106-2221-E-214-038, MOST 107-2221-E-214-017, MOST 108-2923-I-390-001-MY3, MOST 109-2314-B-390-001-MY3, MOST 110-2221-E-214-012 and the Zuoying Branch of Kaohsiung Armed Forces General Hospital under Contract no. KAFGH-ZY_A_111003.

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