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Antioxidant and Immunomodulatory Effects of Laminaria japonica Water Extract

다시마 추출물의 항산화와 염증 조절 효과

  • Cui, Jiamei (Department of Food Science and Nutrition, and Korea-China Joint R&D center on Plant-derived polysaccharide, Jeju National University) ;
  • Kim, Eunyoung (Department of Food Science and Nutrition, and Korea-China Joint R&D center on Plant-derived polysaccharide, Jeju National University) ;
  • Zhang, Guiguo (College of Animal Science and Technology, and Korea-China Joint R&D center on Plant-derived polysaccharide, Shandong Provincial Key Laboratory of Animal Biotechnology and Disease Control and Prevention, Shandong Agricultural University) ;
  • Lee, Yunkyoung (Department of Food Science and Nutrition, and Korea-China Joint R&D center on Plant-derived polysaccharide, Jeju National University)
  • 최가미 (제주대학교 식품영양학과, 한-중 식품유래 기능성 다당류 공동 연구개발센터) ;
  • 김은영 (제주대학교 식품영양학과, 한-중 식품유래 기능성 다당류 공동 연구개발센터) ;
  • ;
  • 이윤경 (제주대학교 식품영양학과, 한-중 식품유래 기능성 다당류 공동 연구개발센터)
  • Received : 2022.08.23
  • Accepted : 2022.08.31
  • Published : 2022.10.30

Abstract

Laminaria japonica is a type of brown algae widely consumed in Asian countries and contains many essential nutrients and exhibits anti-obesity, antioxidant, and anti-inflammatory effects. In this study, the antioxidant and immunomodulatory effects of a Laminaria japonica water extract (LJE) were investigated using an in vitro model. Mean total polyphenol content of LJE was 2.16±0.11 ㎍ GAE/mg, and LJE dose-dependently inhibited ABTS radical activity but did not scavenge DPPH radicals. In addition, LJE enhanced nitric oxide (NO) production and upregulated the mRNA expressions of proinflammatory cytokines (i.e., tumor necrosis factor-α and interleukin-6) in RAW 264.7 cells. On the other hand, LJE inhibited NO production and downregulated proinflammatory cytokine mRNA levels in endotoxin-stimulated RAW 264.7 cells. Thus, our data show that LJE has moderate antioxidant activity and biphasic immunomodulatory effects on RAW 264.7 cells. In summary, the study indicates that LJE has potential therapeutic use as a novel biphasic immuno-modulator.

Keywords

Acknowledgement

본 연구는 the National Key R&D Program of China-Korea Cooperative Project 2019YFE0107700와 한국연구재단 NRF-2019K1A3A1A20081146의 지원에 의해 수행되었으며, 이에 감사드립니다.

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