Journal of the Korean Society of Food Culture (한국식생활문화학회지)

Korean Society of Food Culture (한국식생활문화학회)
권호 표지
DOI QR코드

DOI QR Code

Antioxidant and Immunomodulatory Effects of Laminaria japonica Water Extract

다시마 추출물의 항산화와 염증 조절 효과

  • Cui, Jiamei (Department of Food Science and Nutrition, and Korea-China Joint R&D center on Plant-derived polysaccharide, Jeju National University) ;
  • Kim, Eunyoung (Department of Food Science and Nutrition, and Korea-China Joint R&D center on Plant-derived polysaccharide, Jeju National University) ;
  • Zhang, Guiguo (College of Animal Science and Technology, and Korea-China Joint R&D center on Plant-derived polysaccharide, Shandong Provincial Key Laboratory of Animal Biotechnology and Disease Control and Prevention, Shandong Agricultural University) ;
  • Lee, Yunkyoung (Department of Food Science and Nutrition, and Korea-China Joint R&D center on Plant-derived polysaccharide, Jeju National University)
  • 최가미 (제주대학교 식품영양학과, 한-중 식품유래 기능성 다당류 공동 연구개발센터) ;
  • 김은영 (제주대학교 식품영양학과, 한-중 식품유래 기능성 다당류 공동 연구개발센터) ;
  • ;
  • 이윤경 (제주대학교 식품영양학과, 한-중 식품유래 기능성 다당류 공동 연구개발센터)
  • Received : 2022.08.23
  • Accepted : 2022.08.31
  • Published : 2022.10.30
Download PDF
⟨ Previous Next ⟩

Abstract

Laminaria japonica is a type of brown algae widely consumed in Asian countries and contains many essential nutrients and exhibits anti-obesity, antioxidant, and anti-inflammatory effects. In this study, the antioxidant and immunomodulatory effects of a Laminaria japonica water extract (LJE) were investigated using an in vitro model. Mean total polyphenol content of LJE was 2.16±0.11 ㎍ GAE/mg, and LJE dose-dependently inhibited ABTS radical activity but did not scavenge DPPH radicals. In addition, LJE enhanced nitric oxide (NO) production and upregulated the mRNA expressions of proinflammatory cytokines (i.e., tumor necrosis factor-α and interleukin-6) in RAW 264.7 cells. On the other hand, LJE inhibited NO production and downregulated proinflammatory cytokine mRNA levels in endotoxin-stimulated RAW 264.7 cells. Thus, our data show that LJE has moderate antioxidant activity and biphasic immunomodulatory effects on RAW 264.7 cells. In summary, the study indicates that LJE has potential therapeutic use as a novel biphasic immuno-modulator.

Keywords

Acknowledgement

본 연구는 the National Key R&D Program of China-Korea Cooperative Project 2019YFE0107700와 한국연구재단 NRF-2019K1A3A1A20081146의 지원에 의해 수행되었으며, 이에 감사드립니다.

References

  1. Baek SH, Lee HJ, Lee CH, Nam TJ, Lee SG. 2019. Change of fucoxanthin and total antioxidant capacities of Saccharina japonica during the drying process. Korean J. Food Sci. Technol., 51:524-530
  2. Cho JY, Bang MA. 2004. Hypoglycemic and antioxidative effects of dietary sea-tangle extracts supplementation in streptozotocin-induced diabetic rats. Korean J. Nutr., 37:5-14
  3. Choi JS, Shin SH, Ha YM, Kim YC, Kim TB, Park SM, Choi IS, Song HJ, Choi YJ. 2008. Mineral Contents and Physiological Activities of Dried Sea Tangle (Laminaria japonica) Collected from Gijang and Wando in Korea. J. Life Sci., 18:474-481 https://doi.org/10.5352/JLS.2008.18.4.474
  4. Cui J. 2021. Comparison of biological characteristics and immunomodulatory effect between purified polysaccharides and water extract from Laminaria japonica, Master's degree thesis, Jeju National University, Korea, pp 26-27
  5. Cui J, Wang Y, Kim E, Zhang C, Zhang G, Lee Y. 2022. Structural characteristics and immunomodulatory effects of a long-chain polysaccharide from Laminaria Japonica. Front. Nutr., 762595:1-16
  6. Fang Q, Wang JF, Zha XQ, Cui SH, Cao L, Luo JP. 2015. Immunomodulatory activity on macrophage of a purified polysaccharide extracted from Laminaria japonica. Carbohydr. Polym., 134:66-73 https://doi.org/10.1016/j.carbpol.2015.07.070
  7. Folin O, Denis W. 1912. On phosphotungstic-phosphomolybdic compounds as color reagents. J. Biol. Chem., 12:239-243 https://doi.org/10.1016/S0021-9258(18)88697-5
  8. Hwang YJ, Chae I, Lee Y. 2017. Anti-inflammatory effects of fermented Laminaria japonica and Hizikia fusiforme water extracts with probiotics in LPS-stimulated RAW264. 7 macrophage cell line. J. East Asian Soc. Diet. Life, 27:1-8 https://doi.org/10.17495/easdl.2017.2.27.1.1
  9. Jung KI, Kim BK, Kang JH, Oh GH, Kim IK, Kim M. 2019. Antioxidant and anti-inflammatory activities of water and the fermentation liquid of sea tangle (Saccharina japonica). J. Life Sci., 29:596-606
  10. Kang SY, Kim E, Kang I, Lee M, Lee Y. 2018. Anti-diabetic effects and anti-inflammatory effects of Laminaria japonica and Hizikia fusiforme in skeletal muscle: in vitro and in vivo model. Nutr., 10:1-12
  11. Kim JH, Kang HM, Lee SH, Lee JY, Park LY. 2015. Antioxidant and α-glucosidase inhibition activity of seaweed extracts. Korean J. Food Preserv., 22:290-296 https://doi.org/10.11002/kjfp.2015.22.2.290
  12. Kim JH, Lee DS, Lim CW, Park HY, Park JH. 2002. Antibacterial activity of sea-mustard, Laminaria japonica extracts on the cariogenic bacteria, Streptococcus mutans. Korean J. Fish. Aquat. Sci., 35:191-195
  13. Kim KS. 2004. Clinical Use of Non-Steroidal Anti-Inflammatory Drugs. Korean J. Otorhinolaryngol.-Head Neck Surg., 47:91-98
  14. Kim YS, Kang CO, Kim MH, Cha WS, Shin HJ. 2011. Contents of water extract for Laminaria japonica and its antioxidant activity. Korean Soc. Biotechnol. Bioeng. J., 26:112-118
  15. Kwak CS, Kim S, Lee MS. 2005. The correlation of antioxidative effects of 5 Korean common edible seaweeds and total polyphenol content. J. Korean Soc. Food Sci. Nutr., 34:1143-1150 https://doi.org/10.3746/jkfn.2005.34.8.1143
  16. Lee HS, Song MW, Kim KT, Hong WS, Paik HD. 2021. Antioxidant effect and sensory evaluation of yogurt supplemented with hydroponic ginseng root extract. Foods, 639:1-10
  17. Lee NY. 2013. Antioxidant effect and tyrosinase inhibition activity of seaweeds ethanol extracts. J. Korean Soc. Food Sci. Nutr., 42:1893-1898 https://doi.org/10.3746/jkfn.2013.42.12.1893
  18. Li HY, Yi YL, Guo S, Zhang F, Yan H, Zhan ZL, Zhu Y, Duan JA. 2022. Isolation, structural characterization and bioactivities of polysaccharides from Laminaria japonica: A review. Food Chem., 370:1-10
  19. Lin H-TV, Lu WJ, Tsai GJ, Chou CT, Hsiao HI, Hwang PA. 2016. Enhanced anti-inflammatory activity of brown seaweed Laminaria japonica by fermentation using Bacillus subtilis. Process Biochem., 51:1945-1953 https://doi.org/10.1016/j.procbio.2016.08.024
  20. Liu F, Zhang X, Ling P, Liao J, Zhao M, Mei L, Shao H, Jiang P, Song Z, Chen Q. 2017. Immunomodulatory effects of xanthan gum in LPS-stimulated RAW 264.7 macrophages. Carbohydr. Polym., 169:65-74 https://doi.org/10.1016/j.carbpol.2017.04.003
  21. Na HS, Kim JY, Park JS, Choi GC, Yang SI, Lee JH, Cho JY, Ma SJ. 2014. Characteristics of marine algae extracts using subcritical water extract method. Korean J. Food Preserv., 21:62-68 https://doi.org/10.11002/kjfp.2014.21.1.62
  22. Oh JH, Lee Y. 2015. Effects of water and ethanol extracts from four types of domestic seaweeds on cell differentiation in 3T3-L1 cell line. J. East Asian Soc. Diet. Life, 25:990-998 https://doi.org/10.17495/easdl.2015.12.25.6.990
  23. Park JN, Ali-Nehari A, Woo HC, Chun BS. 2012. Thermal stabilities of polyphenols and fatty acids in Laminaria japonica hydrolysates produced using subcritical water. Korean J. Chem. Eng., 29:1604-1609 https://doi.org/10.1007/s11814-012-0051-y
  24. Park SK, Park SJ, Park SM, Cho IJ, Park CI, Kim YW, Kim SC. 2013. Inhibition of acute phase inflammation by Laminaria japonica through regulation of iNOS-NF-κB pathway. Evid. Based Complement. Alternat. Med., 439498:1-11
  25. Peng FH, Zha XQ, Cui SH, Asghar MN, Pan LH, Wang JH, Luo JP. 2015. Purification, structure features and antiatherosclerosis activity of a Laminaria japonica polysaccharide. Int. J. Biol. Macromol., 81:926-935 https://doi.org/10.1016/j.ijbiomac.2015.09.027
  26. Re R, Pellegrini N, Proteggente A, Pannala A, Yang M, and Rice-Evans C. 1999. Antioxidant activity applying an improved ABTS radical cation decolorization assay. Free Radic. Biol. Med., 26:1231-1237 https://doi.org/10.1016/S0891-5849(98)00315-3
  27. Soufli I, Toumi R, Rafa H, Touil-Boukoffa C. 2016. Overview of cytokines and nitric oxide involvement in immunopathogenesis of inflammatory bowel diseases. World J. Gastrointest. Pharmcol. Ther., 7:353-360 https://doi.org/10.4292/wjgpt.v7.i3.353
  28. Yang HS, Haj FG, Lee M, Kang I, Zhang G, Lee Y. 2019. Laminaria japonica extract enhances intestinal barrier function by altering inflammatory response and tight junction-related protein in lipopolysaccharide-stimulated Caco-2 cells. Nutr., 11:1-14
  29. Yoo H, Lee D. 2004. Chungkookjang fermentation of mixture of barley, wormwood, sea tangle, and soybean. Korean J. Microbiol., 40:49-53
  30. Zhu Q, Chen J, Li Q, Wang T, Li H. 2016. Antitumor activity of polysaccharide from Laminaria japonica on mice bearing H22 liver cancer. Int. J. Biol. Macromole, 92:156-158 https://doi.org/10.1016/j.ijbiomac.2016.06.090
  31. Pahwa R, Goyal A, Jialal I. Chronic inflammation. 2021. Available from: https://www.ncbi.nlm.nih.gov/books/NBK493173/, [accessed 2022. 08. 22]