Anatomy and Cell Biology

Korean Association of Anatomists (대한해부학회)
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The in vitro analysis of migration and polarity of blastema cells in the extracellular matrix derived from bovine mesenteric in the presence of fibronectin

  • Kamelia Kohannezhad (Department of Biology, Kavian Institute of Higher Education) ;
  • Soroush Norouzi (Department of Biology, Kavian Institute of Higher Education) ;
  • Maryam Tafazoli (Department of Biology, Kavian Institute of Higher Education) ;
  • Safoura Soleymani (Division of Biotechnology, Faculty of Veterinary Medicine, Ferdowsi University of Mashhad) ;
  • Nasser Mahdavi Shahri (Department of Biology, Kavian Institute of Higher Education) ;
  • Amin Tavassoli (Department of Biology, Kavian Institute of Higher Education)
  • Received : 2021.11.23
  • Accepted : 2022.02.28
  • Published : 2022.06.30
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Abstract

Cell migration is an essential process in embryonic development, wound healing, and pathological conditions. Our knowledge of cell migration is often based on the two dimentional evaluation of cell movement, which usually differs from what occurred in vivo. In this study, we investigated cellular migration from blastema tissue toward bovine decellularized mesentery tissue. In this regard, fibronectin (FN) was assessed to confirm cell migration. Therefore, we established a cell migration model using blastema cells migration toward the extracellular matrix derived from bovine mesenteric tissue. A physiochemical decellularization method was utilized based on freeze-thaw cycles and agitation in sodium dodecyl sulfate and Triton X-100 to remove cells from the extracellular matrix (ECM) of bovine mesenteric tissue. These types of matrices were assembled by the rings of blastema tissues originated from the of New Zealand rabbits pinna and cultured in a medium containing FN in different days in vitro, and then they are histologically evaluated, and the expression of the Tenascin C gene is analyzed. By means of tissue staining and after confirmation of the cell removal from mesenteric tissue, polarity, and migration of blastema cells was observed in the interaction site with this matrix. Also, the expression of the Tenascin C gene was assessed on days 15 and 21 following the cell culture process. The results showed that the three dimentional model of cellular migration of blastema cells along with the ECM could be a suitable model for investigating cell behaviors, such as polarity and cell migration in vitro.

Keywords

Acknowledgement

This study was financially supported by the Ferdowsi University of Mashhad and a research grant provided by Ferdowsi University of Mashhad and Kavian Institute of Higher Education.

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