Animal Systematics, Evolution and Diversity

The Korean Society of Systematic Zoology (한국동물분류학회)
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DNA Barcoding for Diophrys quadrinucleata (Ciliophora: Euplotia) from South Korea

  • Chae, Kyu-Seok (Department of Biological Sciences and Bioengineering, Inha University) ;
  • Min, Gi-Sik (Department of Biological Sciences and Bioengineering, Inha University)
  • Received : 2022.05.10
  • Accepted : 2022.09.02
  • Published : 2022.10.31
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Abstract

One marine ciliate, Diophrys quadrinucleata Zhang et al., 2020 was newly recorded from South Korea in this study. We provided morphological diagnosis and images of the Korean D. quadrinucleata population. We determined the small subunit ribosomal DNA (SSU rDNA) and cytochrome oxidase subunit I (CO1) sequence data of D. quadrinucleata, and then the sequences were compared with other Diophrys species. Intra-specific variation between the Korean and type (Chinese) populations was identical in the SSU rDNA, while the inter-specific variations between seven Diophrys species were 0.3-3.8% in the SSU rDNA and 12.6-18.2% in the CO1. In this study, we obtained 18S and CO1 data from species with identified morphology. As the importance of securing 18S and CO1 based on morphology increases in current studies, this study will contribute to ciliate studies.

Keywords

Acknowledgement

This study was supported by research funds from the National Institute of Biological Resources (NIBR), funded by the Ministry of Environment (MOE) of the Republic of Korea (NIBR202102203).

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