Journal of Korean Medicine Rehabilitation (한방재활의학과학회지)

The society of Korean Medicine Rehabilitation (한방재활의학과학회)
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Anti-inflammatory Effects of Naetakbaekryeom-san

내탁백렴산 추출물의 항염증 효능 연구

  • Jung, Min Jae (Department of Korean Medicine Rehabilitation, College of Korean Medicine, Dongshin University) ;
  • Noh, Hui Jeong (Department of Korean Medicine, Rehabilitation, Dongshin University Mokpo Korean Medicine Hospital) ;
  • Choi, Ji Min (Department of Korean Medicine Rehabilitation, College of Korean Medicine, Dongshin University) ;
  • Jeon, Seok Hee (Department of Acupuncture and Moxibustion Medicine, Dongshin University Mokpo Korean Medicine Hospital) ;
  • Kim, Seon Jong (Department of Korean Medicine Rehabilitation, College of Korean Medicine, Dongshin University)
  • 정민재 (동신대학교 한의과대학 한방재활의학교실) ;
  • 노희정 (동신대학교 목포한방병원 한방재활의학과) ;
  • 최지민 (동신대학교 한의과대학 한방재활의학교실) ;
  • 전석희 (동신대학교 목포한방병원 침구과) ;
  • 김선종 (동신대학교 한의과대학 한방재활의학교실)
  • Received : 2022.06.17
  • Accepted : 2022.07.12
  • Published : 2022.10.31
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Abstract

Objectives This study was conducted to confirm the anti-inflammatory effect of Naetakbaekryeom-san (NTB), and whether it could be another treatment for inflammatory diseases. Methods The NTB water extract was extracted with hot water at 100℃ for 2 hours, concentrated at 80℃ under reduced pressure, and used. After 2 hours of pretreatment with NTB and positive control Bay11-7082, nitric oxide (NO), inducible NO synthase (iNOS), interleukin (IL)-6, IL-1𝛽, tumor necrosis factor alpha (TNF-𝛼) were measured in RAW264.7 cells activated with lipopolysaccharides (LPS) 500 ng/mL. After 2 hours of pretreatment with NTB, the anti-inflammatory effect of NTB was evaluated by measuring nuclear factor kappa-light-chain-enhancer of activated B cells (NF-𝜅B) in RAW264.7 cells and 293T cells activated with phorbol 12-myristate 13-acetic acid (PMA) 30 ng/mL. Results In RAW264.7 cells activated with LPS, NTB at concentrations of 0.1, 0.3, and 1.0 mg/mL showed no cytotoxicity, significantly inhibited NO production and inhibition of iNOS expression. TNF-𝛼 cytokine levels was not regulated, but NTB at each concentration inhibited the production of IL-1𝛽 and IL-6, and the effect was higher than that of the positive control Bay11-7082 (20 𝜇M). In PMA-activated RAW264.7 cells and 293T cells, each concentration of NBT decreased the NF-𝜅B transcriptional activity, with the greatest decrease at 1 mg/mL. Conclusions These results demonstrated the anti-inflammatory effect of NTB water extracts, but further studies such as comparison of anti-inflammatory effects and antioxidant effects by NTB component, comparison of effects according to extraction solvents, and clinical studies are needed.

Keywords

Acknowledgement

본 연구는 보건복지부의 재원으로 한국보건산업진흥원의 보건의료기술연구개발사업 지원에 의하여 이루어진 것임(과제고유번호: HI21C1924).

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