통합자연과학논문집 (Journal of Integrative Natural Science)

조선대학교 기초과학연구원 (The Basic Science Institute Chosun University)
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태반 추출물의 자가포식 활성을 통해 산화스트레스에 대한 슈반세포 보호 효과

Protective Effect of Placental Extract against Oxidative Stress through Autophagy Activity in Schwann Cells

  • 임경민 (조선대학교 글로벌바이오융합학과) ;
  • 조광원 (조선대학교 생명과학과) ;
  • 장철호 (전남대학교 의과대학 이비인후과)
  • Lim, GyeongMin (Department of Integrative Biological Science, Chosun University) ;
  • Cho, Gwang-Won (Department of Biology, Chosun University) ;
  • Jang, Chul Ho (Department of Otolaryngology, Chonnam National University Medical School)
  • 투고 : 2022.09.13
  • 심사 : 2022.09.27
  • 발행 : 2022.10.14
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초록

Schwann cells play a critical role for myelination in peripheral nerve system. It also plays an important role in nerve protection and regeneration. In peripheral nerve damage, regeneration is induced by the migration and proliferation of Schwann cells which were promoted by suppressing the oxidative stress. In this study, Human placental extract was prepared by homogenization and estimated its efficacy in RSC96 cells. Placental extract exhibited a protective effect against hydrogen peroxide-induced oxidative stress in RSC96 cells, confirmed by MTT assay. Furthermore, placental extract decreased intracellular ROS against oxidative stress, confirmed by DCFH-DA assay. Autophagy was visualized with Cyto-ID staining to confirm the autophagy activity of placental extracts. The activity of autophagy was confirmed by immunoblot analysis of autophagy flux-associated proteins such as LC3 conversion and SQSTM1 degradation. Thus, we confirmed the antioxidant effect of placental extract to protect RSC96 cells from oxidative stress, and observed that it activated autophagy and restored autophagy flux.

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참고문헌

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