Journal of Integrative Natural Science (통합자연과학논문집)

The Basic Science Institute Chosun University (조선대학교 기초과학연구원)
권호 표지
DOI QR코드

DOI QR Code

Protective Effect of Placental Extract against Oxidative Stress through Autophagy Activity in Schwann Cells

태반 추출물의 자가포식 활성을 통해 산화스트레스에 대한 슈반세포 보호 효과

  • Lim, GyeongMin (Department of Integrative Biological Science, Chosun University) ;
  • Cho, Gwang-Won (Department of Biology, Chosun University) ;
  • Jang, Chul Ho (Department of Otolaryngology, Chonnam National University Medical School)
  • 임경민 (조선대학교 글로벌바이오융합학과) ;
  • 조광원 (조선대학교 생명과학과) ;
  • 장철호 (전남대학교 의과대학 이비인후과)
  • Received : 2022.09.13
  • Accepted : 2022.09.27
  • Published : 2022.10.14
Download PDF
⟨ Previous Next ⟩

Abstract

Schwann cells play a critical role for myelination in peripheral nerve system. It also plays an important role in nerve protection and regeneration. In peripheral nerve damage, regeneration is induced by the migration and proliferation of Schwann cells which were promoted by suppressing the oxidative stress. In this study, Human placental extract was prepared by homogenization and estimated its efficacy in RSC96 cells. Placental extract exhibited a protective effect against hydrogen peroxide-induced oxidative stress in RSC96 cells, confirmed by MTT assay. Furthermore, placental extract decreased intracellular ROS against oxidative stress, confirmed by DCFH-DA assay. Autophagy was visualized with Cyto-ID staining to confirm the autophagy activity of placental extracts. The activity of autophagy was confirmed by immunoblot analysis of autophagy flux-associated proteins such as LC3 conversion and SQSTM1 degradation. Thus, we confirmed the antioxidant effect of placental extract to protect RSC96 cells from oxidative stress, and observed that it activated autophagy and restored autophagy flux.

Keywords

References

  1. J. Du, G. Zhen, H. Chen, S. Zhang, L. Qing, X. Yang, G. Lee, H. Q. Mao, and X. Jia. "Optimal Electrical Stimulation Boosts Stem Cell Therapy in Nerve Regeneration." [In eng]. Biomaterials 181 (Oct 2018): 347-59. https://doi.org/10.1016/j.biomaterials.2018.07.015
  2. B. Liu, W. Xin, J. R. Tan, R. P. Zhu, T. Li, D. Wang, S. S. Kan, et al. "Myelin Sheath Structure and Regeneration in Peripheral Nerve Injury Repair." [In eng]. Proc Natl Acad Sci U S A 116, no. 44 (Oct 29 2019): 22347-52. https://doi.org/10.1073/pnas.1910292116
  3. H. S. Loring, and P. R. Thompson. "Emergence of Sarm1 as a Potential Therapeutic Target for Wallerian-Type Diseases." [In eng]. Cell Chem Biol 27, no. 1 (Jan 16 2020): 1-13. https://doi.org/10.1016/j.chembiol.2019.11.002
  4. Y. Shen, W. Jian, J. Li, T. Dai, B. Bao, and H. Nie. "Bilateral Wallerian Degeneration of the Middle Cerebellar Peduncles Secondary to Pontine Infarction: A Case Series." [In eng]. J Neurol Sci 388 (May 15 2018): 182-85. https://doi.org/10.1016/j.jns.2018.03.027
  5. Y. J. Chen, S. A. Nabavizadeh, A. Vossough, S. Kumar, L. A. Loevner, and S. Mohan. "Wallerian Degeneration Beyond the Corticospinal Tracts: Conventional and Advanced Mri Findings." [In eng]. J Neuroimaging 27, no. 3 (May 2017): 272-80. https://doi.org/10.1111/jon.12404
  6. H. M. Chang, C. H. Liu, W. M. Hsu, L. Y. Chen, H. P. Wang, T. H. Wu, K. Y. Chen, W. H. Ho, and W. C. Liao. "Proliferative Effects of Melatonin on Schwann Cells: Implication for Nerve Regeneration Following Peripheral Nerve Injury." [In eng]. J Pineal Res 56, no. 3 (Apr 2014): 322-32. https://doi.org/10.1111/jpi.12125
  7. L. Zhang, D. Johnson, and J. A. Johnson. "Deletion of Nrf2 Impairs Functional Recovery, Reduces Clearance of Myelin Debris and Decreases Axonal Remyelination after Peripheral Nerve Injury." [In eng]. Neurobiol Dis 54 (Jun 2013): 329-38. https://doi.org/10.1016/j.nbd.2013.01.003
  8. C. Lanza, S. Raimondo, L. Vergani, N. Catena, F. Senes, P. Tos, and S. Geuna. "Expression of Antioxidant Molecules after Peripheral Nerve Injury and Regeneration." [In eng]. J Neurosci Res 90, no. 4 (Apr 2012): 842-8. https://doi.org/10.1002/jnr.22778
  9. Y. Qian, Q. Han, X. Zhao, J. Song, Y. Cheng, Z. Fang, Y. Ouyang, W. E. Yuan, and C. Fan. "3d Melatonin Nerve Scaffold Reduces Oxidative Stress and Inflammation and Increases Autophagy in Peripheral Nerve Regeneration." [In eng]. J Pineal Res 65, no. 4 (Nov 2018): e12516. https://doi.org/10.1111/jpi.12516
  10. W. M. Renno, L. Benov, and K. M. Khan. "Possible Role of Antioxidative Capacity of (-)-Epigallocatechin-3-Gallate Treatment in Morphological and Neurobehavioral Recovery after Sciatic Nerve Crush Injury." [In eng]. J Neurosurg Spine 27, no. 5 (Nov 2017): 593-613. https://doi.org/10.3171/2016.10.SPINE16218
  11. T. Lamark, and T. Johansen. "Aggrephagy: Selective Disposal of Protein Aggregates by Macroautophagy." [In eng]. Int J Cell Biol 2012 (2012): 736905. https://doi.org/10.1155/2012/736905
  12. H. R. Yun, Y. H. Jo, J. Kim, Y. Shin, S. S. Kim, and T. G. Choi. "Roles of Autophagy in Oxidative Stress." [In eng]. Int J Mol Sci 21, no. 9 (May 6 2020).
  13. H. Weidberg, E. Shvets, and Z. Elazar. "Biogenesis and Cargo Selectivity of Autophagosomes." [In eng]. Annu Rev Biochem 80 (2011): 125-56. https://doi.org/10.1146/annurev-biochem-052709-094552
  14. S. C. Lai, and R. J. Devenish. "Lc3-Associated Phagocytosis (Lap): Connections with Host Autophagy." [In eng]. Cells 1, no. 3 (Jul 30 2012): 396-408. https://doi.org/10.3390/cells1030396
  15. W. J. Liu, L. Ye, W. F. Huang, L. J. Guo, Z. G. Xu, H. L. Wu, C. Yang, and H. F. Liu. "P62 Links the Autophagy Pathway and the Ubiqutin-Proteasome System Upon Ubiquitinated Protein Degradation." [In eng]. Cell Mol Biol Lett 21 (2016): 29. https://doi.org/10.1186/s11658-016-0031-z
  16. G. Filomeni, D. De Zio, and F. Cecconi. "Oxidative Stress and Autophagy: The Clash between Damage and Metabolic Needs." [In eng]. Cell Death Differ 22, no. 3 (Mar 2015): 377-88. https://doi.org/10.1038/cdd.2014.150
  17. Leo Donnelly, and Gillian Campling. "Functions of the Placenta." Anaesthesia & intensive care medicine 15, no. 3 (2014): 136-39. https://doi.org/10.1016/j.mpaic.2014.01.004
  18. Y Karasawa, Y Iwasaki, S Kagawa, M Saito, Y Iwasaki, Y Kimura, K Kobayashi, and M Tsuyuguchi. "Clinical Treatment Test of Melsmon on Menopausal Disorder." Med. Treat. 1981; 9 (3): 1 10 (1981).
  19. S. K. Tiwary, D. Shukla, A. K. Tripathi, S. Agrawal, M. K. Singh, and V. K. Shukla. "Effect of Placental-Extract Gel and Cream on Non-Healing Wounds." [In eng]. J Wound Care 15, no. 7 (Jul 2006): 325-8. https://doi.org/10.12968/jowc.2006.15.7.26937
  20. J. Y. Choi, K. Lee, S. M. Lee, S. H. Yoo, S. G. Hwang, J. Y. Choi, S. W. Lee, et al. "Efficacy and Safety of Human Placental Extract for Alcoholic and Nonalcoholic Steatohepatitis: An Open-Label, Randomized, Comparative Study." [In eng]. Biol Pharm Bull 37, no. 12 (2014): 1853-9. https://doi.org/10.1248/bpb.b13-00979
  21. M. H. Kong, E. J. Lee, S. Y. Lee, S. J. Cho, Y. S. Hong, and S. B. Park. "Effect of Human Placental Extract on Menopausal Symptoms, Fatigue, and Risk Factors for Cardiovascular Disease in Middle-Aged Korean Women." [In eng]. Menopause 15, no. 2 (Mar-Apr 2008): 296-303. https://doi.org/10.1097/gme.0b013e3181405b74
  22. J. W. Hong, W. J. Lee, S. B. Hahn, B. J. Kim, and D. H. Lew. "The Effect of Human Placenta Extract in a Wound Healing Model." [In eng]. Ann Plast Surg65, no. 1 (Jul 2010): 96-100.
  23. S. Togashi, N. Takahashi, M. Iwama, S. Watanabe, K. Tamagawa, and T. Fukui. "Antioxidative Collagen-Derived Peptides in Human-Placenta Extract." [In eng]. Placenta 23, no. 6 (Jul 2002): 497-502. https://doi.org/10.1053/plac.2002.0833
  24. Y. K. Lee, H. H. Chung, and S. B. Kang. "Efficacy and Safety of Human Placenta Extract in Alleviating Climacteric Symptoms: Prospective, Randomized, Double-Blind, Placebo-Controlled Trial." [In eng]. J Obstet Gynaecol Res 35, no. 6 (Dec 2009): 1096-101. https://doi.org/10.1111/j.1447-0756.2009.01066.x
  25. L. A. Cole. "Biological Functions of Hcg and Hcg-Related Molecules." [In eng]. Reprod Biol Endocrinol 8 (Aug 24 2010): 102. https://doi.org/10.1186/1477-7827-8-102
  26. D. H. Bak, J. Na, S. I. Im, C. T. Oh, J. Y. Kim, S. K. Park, H. J. Han, et al. "Antioxidant Effect of Human Placenta Hydrolysate against Oxidative Stress on Muscle Atrophy." [In eng]. J Cell Physiol 234, no. 2 (Feb 2019): 1643-58. https://doi.org/10.1002/jcp.27034