Clinical and Experimental Reproductive Medicine

The Korean Society for Reproductive Medicine (대한생식의학회)
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Sperm DNA fragmentation in consecutive ejaculates from patients with cancer for sperm cryopreservation

  • Kim, Seul Ki (Department of Obstetrics and Gynecology, Seoul National University Bundang Hospital) ;
  • Paik, Haerin (Department of Obstetrics and Gynecology, Seoul National University Bundang Hospital) ;
  • Lee, Jung Ryeol (Department of Obstetrics and Gynecology, Seoul National University Bundang Hospital) ;
  • Jee, Byung Chul (Department of Obstetrics and Gynecology, Seoul National University Bundang Hospital)
  • Received : 2022.03.04
  • Accepted : 2022.06.13
  • Published : 2022.09.30
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Abstract

Objective: This prospective consecutive study investigated the variation in sperm DNA fragmentation (SDF) in multiple semen samples from patients with cancer. Methods: Eighty-one patients with various cancers underwent multiple semen collections on 3 consecutive days for sperm cryopreservation prior to cancer treatment. A commercial Halosperm kit was used to measure SDF. Within- and between-subject coefficients of variation were estimated via random-effects analysis of variance to assess the consistency of semen parameters and SDF. Intraclass correlation coefficients (ICCs) were calculated to assess the magnitude of the between-subject component of variance relative to the total variance. Results: The volume of semen in the day-2 and day-3 samples was significantly lower compared with the day-1 sample. Most parameters showed high ICC values, suggesting that within-subject fluctuations were small relative to the between-subject variability. The highest ICC values were identified for the SDF (ICC, 0.68; 95% confidence interval [CI], 0.45-0.84) and semen volume (ICC, 0.67; 95% CI, 0.45-0.84). Conclusion: Our findings showed that repeated ejaculates from patients with cancer had stable SDF levels.

Keywords

Acknowledgement

The authors thank the Division of Statistics in the Medical Research Collaborating Center at Seoul National University Bundang Hospital for their statistical analyses.

References

  1. Practice Committee of the American Society for Reproductive Medicine. Fertility preservation in patients undergoing gonadotoxic therapy or gonadectomy: a committee opinion. Fertil Steril 2019;112:1022-33. https://doi.org/10.1016/j.fertnstert.2019.09.013
  2. Oktay K, Harvey BE, Partridge AH, Quinn GP, Reinecke J, Taylor HS, et al. Fertility preservation in patients with cancer: ASCO clinical practice guideline update. J Clin Oncol 2018;36:1994-2001. https://doi.org/10.1200/JCO.2018.78.1914
  3. World Health Organization. WHO laboratory manual for the examination and processing of human semen. 5th ed. Geneva: Department of Reproductive Health and Research, World Health Organization; 2010.
  4. Loren AW, Mangu PB, Beck LN, Brennan L, Magdalinski AJ, Partridge AH, et al. Fertility preservation for patients with cancer: American Society of Clinical Oncology clinical practice guideline update. J Clin Oncol 2013;31:2500-10. https://doi.org/10.1200/JCO.2013.49.2678
  5. Kim SK, Lee JM, Jee BC, Suh CS, Kim SH. Semen quality of consecutive ejaculates from cancer patients for fertility preservation. Fertil Steril 2014;102:1124-9.e3. https://doi.org/10.1016/j.fertnstert.2014.06.045
  6. Kim SM, Kim SK, Jee BC, Kim SH. Effect of sperm DNA fragmentation on embryo quality in normal responder women in in vitro fertilization and intracytoplasmic sperm injection. Yonsei Med J 2019;60:461-6. https://doi.org/10.3349/ymj.2019.60.5.461
  7. Choi HY, Kim SK, Kim SH, Choi YM, Jee BC. Impact of sperm DNA fragmentation on clinical in vitro fertilization outcomes. Clin Exp Reprod Med 2017;44:224-31. https://doi.org/10.5653/cerm.2017.44.4.224
  8. McQueen DB, Zhang J, Robins JC. Sperm DNA fragmentation and recurrent pregnancy loss: a systematic review and meta-analysis. Fertil Steril 2019;112:54-60.e3. https://doi.org/10.1016/j.fertnstert.2019.03.003
  9. Dutta S, Henkel R, Agarwal A. Comparative analysis of tests used to assess sperm chromatin integrity and DNA fragmentation. Andrologia 2021;53:e13718.
  10. Alvarez C, Castilla JA, Martinez L, Ramirez JP, Vergara F, Gaforio JJ. Biological variation of seminal parameters in healthy subjects. Hum Reprod 2003;18:2082-8. https://doi.org/10.1093/humrep/deg430
  11. Carlsen E, Petersen JH, Andersson AM, Skakkebaek NE. Effects of ejaculatory frequency and season on variations in semen quality. Fertil Steril 2004;82:358-66. https://doi.org/10.1016/j.fertnstert.2004.01.039
  12. Keel BA. Within- and between-subject variation in semen parameters in infertile men and normal semen donors. Fertil Steril 2006;85:128-34. https://doi.org/10.1016/j.fertnstert.2005.06.048
  13. Kim SW, Nho EJ, Lee JY, Jee BC. Specific tail swelling pattern in hypo-osmotic solution as a predictor of DNA fragmentation status in human spermatozoa. Clin Exp Reprod Med 2019;46:147-51. https://doi.org/10.5653/cerm.2019.00458
  14. Williams DH 4th, Karpman E, Sander JC, Spiess PE, Pisters LL, Lipshultz LI. Pretreatment semen parameters in men with cancer. J Urol 2009;181:736-40.
  15. van Casteren NJ, Boellaard WP, Romijn JC, Dohle GR. Gonadal dysfunction in male cancer patients before cytotoxic treatment. Int J Androl 2010;33:73-9. https://doi.org/10.1111/j.1365-2605.2009.00956.x
  16. Bungum M, Humaidan P, Axmon A, Spano M, Bungum L, Erenpreiss J, et al. Sperm DNA integrity assessment in prediction of assisted reproduction technology outcome. Hum Reprod 2007;22:174-9. https://doi.org/10.1093/humrep/del326
  17. Evenson DP, Wixon R. Data analysis of two in vivo fertility studies using sperm chromatin structure assay-derived DNA fragmentation index vs. pregnancy outcome. Fertil Steril 2008;90:1229-31. https://doi.org/10.1016/j.fertnstert.2007.10.066
  18. Tandara M, Bajic A, Tandara L, Bilic-Zulle L, Sunj M, Kozina V, et al. Sperm DNA integrity testing: big halo is a good predictor of embryo quality and pregnancy after conventional IVF. Andrology 2014;2:678-86. https://doi.org/10.1111/j.2047-2927.2014.00234.x
  19. Wijchman JG, de Wolf BT, Graafe R, Arts EG. Variation in semen parameters derived from computer-aided semen analysis, within donors and between donors. J Androl 2001;22:773-80.
  20. Sivanarayana T, Ravi Krishna C, Jaya Prakash G, Krishna KM, Madan K, Sudhakar G, et al. Sperm DNA fragmentation assay by sperm chromatin dispersion (SCD): correlation between DNA fragmentation and outcome of intracytoplasmic sperm injection. Reprod Med Biol 2013;13:87-94.
  21. Belloc S, Benkhalifa M, Cohen-Bacrie M, Dalleac A, Chahine H, Amar E, et al. Which isolated sperm abnormality is most related to sperm DNA damage in men presenting for infertility evaluation. J Assist Reprod Genet 2014;31:527-32. https://doi.org/10.1007/s10815-014-0194-3
  22. Al Omrani B, Al Eisa N, Javed M, Al Ghedan M, Al Matrafi H, Al Sufyan H. Associations of sperm DNA fragmentation with lifestyle factors and semen parameters of Saudi men and its impact on ICSI outcome. Reprod Biol Endocrinol 2018;16:49. https://doi.org/10.1186/s12958-018-0369-3