Journal of Microbiology and Biotechnology

The Korean Society for Microbiology and Biotechnology (한국미생물·생명공학회)
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Prevalence and Genetic Characteristics of Japanese Encephalitis Virus among Mosquitoes and Pigs in Hunan Province, China from 2019 to 2021

  • Tang, Qiwu (Hunan Biological and Electromechanical Polytechnic) ;
  • Deng, Zaofu (Hunan Biological and Electromechanical Polytechnic) ;
  • Tan, Shengguo (Hunan Biological and Electromechanical Polytechnic) ;
  • Song, Guo (Animal Husbandry and Fishery Bureau of Ningyuan) ;
  • Zhang, Hai (Animal Epidemic Prevention Station of Xiangxi Autonomous Prefecture) ;
  • Ge, Lingrui (Hunan Biological and Electromechanical Polytechnic)
  • Received : 2022.07.31
  • Accepted : 2022.08.22
  • Published : 2022.09.28
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Abstract

Japanese encephalitis virus (JEV), the causative agent of Japanese encephalitis (JE), is an importantly zoonotic, vector-borne virus widely prevalent in Asia. Although JE has been well controlled in China, its prevalence remains a huge threat to the pig industry as well as human health. Herein, we report on our molecular and serological investigations of JEV among pigs from different regions in Hunan Province of China from 2019 to 2021. Collectively, 19.27% (583/3026, 95% Confidential Interval (CI) 17.86-20.68) of sampled pigs were positive for JEV IgG antibody as revealed by indirect enzyme-linked immunosorbent assay, and the seroprevalence of JEV among pigs was significantly associated with the development stage and breeding scale (p < 0.01). Meanwhile, 10.99% (42/382, 95% CI 7.86-14.13) of tissue samples of pigs with suspected clinical symptoms of JE and 23.44% (15/64, 95% CI 13.06-33.82) of mosquito batches were JEV-positive via reverse polymerase chain reaction. In addition, the complete E gene sequences of 14 JEV strains identified in this study were amplified and sequenced. Phylogenetic analysis showed that all 14 JEV strains belonged to genotype I-b and displayed a distinct genetic relationship to the present JEV vaccine strain (SA14-14-2). In conclusion, our results revealed not only the severe prevalence of JEV in Hunan Province, but also that JEV I-b might be the predominant genotype in Hunan Province, suggesting therefore that effective measures for JE control are urgently needed.

Keywords

Acknowledgement

This study was supported by the Hunan National Science Foundation Youth Fund Project (Grant No. 2018JJ3265), the "Scientific Research Project" provided by Hunan Biological and Electromechanical Polytechnic (Grant No. 17JZ17), the "College Program" provided by Hunan Biological and Electromechanical Polytechnic Institute (Grant No. 20TD06), and the "Backbone Teacher Project" provided by Hunan Biological and Electromechanical Polytechnic Institute.

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