Biomedical Science Letters (대한의생명과학회지)

The Korean Society for Biomedical Laboratory Sciences (대한의생명과학회)
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Anti-inflammatory and Anti-oxidant Activities of Aster Scaber Ethanol Extract

  • Saba, Evelyn (Department of Veterinary Biomedical Sciences, Faculty of Veterinary and Animal Sciences, Pir Mehr Ali Shah Arid Agriculture University) ;
  • Je, Nayeong (Department of Veterinary Medicine, College of Veterinary Medicine, Kyungpook National University) ;
  • Song, Ji Eun (Department of Veterinary Medicine, College of Veterinary Medicine, Kyungpook National University) ;
  • Shi, Sangwoo (Department of Veterinary Medicine, College of Veterinary Medicine, Kyungpook National University) ;
  • Lee, Juho (Department of Veterinary Medicine, College of Veterinary Medicine, Kyungpook National University) ;
  • Jung, Oneyoung (Department of Veterinary Medicine, College of Veterinary Medicine, Kyungpook National University) ;
  • Han, Beom Jun (Department of Veterinary Medicine, College of Veterinary Medicine, Kyungpook National University) ;
  • Lee, Soo Young (Department of Veterinary Medicine, College of Veterinary Medicine, Kyungpook National University) ;
  • Park, Jongwon (Department of Veterinary Medicine, College of Veterinary Medicine, Kyungpook National University) ;
  • Lee, Yuan Yee (Department of Animal and Avian Sciences, University of Maryland) ;
  • Rhee, Man Hee (Department of Veterinary Medicine, College of Veterinary Medicine, Kyungpook National University)
  • Received : 2022.08.10
  • Accepted : 2022.09.14
  • Published : 2022.09.30
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Abstract

In mountainous regions, wild herbs which can also be edible in nature for humans and animals possess a wide array of biologically diversified properties. It is because of the fact that due to the cold weather of mountains; they are enriched in certain kinds of phytochemicals such as anti-oxidants, anti-inflammatory and many more. One such kind of an herb is Aster scaber (AS) in Korean. It is a widely cultivated culinary herb in Korean peninsula and used as a side dish in Korean culinary cuisine. In view of its extensive use in cuisine, we geared to unravel the anti-oxidant and anti-inflammatory effects of AS in murine alveolar macrophage cell line (MH-S). 2,2'-Azino-bis-3-ethylbenzothiazoline-6-sulfonic acid (ABTS) and 2,2-diphenyl-1-picryl-hydrazyl-hydrate (DPPH) assays revealed a dose dependent (7.8~1,000 ㎍/mL) inhibition of oxidation by AS 70% ethanol (ASE) extract as compared to Trolox and Ascorbic acid respectively. Nitric oxide assay (NO) showed a dose dependent decrease (5~40 ㎍/mL) in MH-S cells with ASE when stimulated with Coal Fly Ash (CFA). Moreover, this dose for NO reduction was also found to be least cytotoxic for cells as determined by cellular viability (MTT) assay. The gene expression of pro-inflammatory mediators (iNOS and COX-2) and cytokines (IL-6 and IL-1β) and were also dose dependently inhibited by ASE in MH-S cells through RT-PCR. Therefore, in light of these findings, AS exhibited a strong anti-oxidant and anti-inflammatory agent. These results also justify the extensive use of this mountainous herb in culinary practices for beneficial effects on human health.

Keywords

Acknowledgement

This work was supported by the National Research Foundation of Korea (NRF) grant funded by the Korea government (MSIT) (No. 2022R1A2C1012963).

References

  1. Andree BPJ. Incidence of Covid-19 and connections with air pollution exposure: Evidence from the netherlands. medRxiv 2020. 04. 27. 20081562.
  2. Azab A, Nassar A, Azab AN. Anti-inflammatory activity of natural products. Molecules. 2016. 21: 1321. https://doi.org/10.3390/molecules21101321
  3. Choi JH, Park YH, Lee IS, Lee SP, Yu MH. Antioxidant activity and inhibitory effect of aster scaber thunb. Extract on adipocyte differentiation in 3t3-l1 cells. Korean J Food Sci Technol. 2013. 45: 356-363. https://doi.org/10.9721/KJFST.2013.45.3.356
  4. Choi YE, Choi SI, Han X, Men X, Jang GW, Kwon HY, Kang SR, Han JS, Lee OH. Radical scavenging-linked anti-adipogenic activity of aster scaber ethanolic extract and its bioactive compound. Antioxidants (Basel). 2020. 9: 1290. https://doi.org/10.3390/antiox9121290
  5. Chung MJ, Lee S, Park YI, Lee J, Kwon KH. Neuroprotective effects of phytosterols and flavonoids from cirsium setidens and aster scaber in human brain neuroblastoma sk-n-sh cells. Life Sci. 2016. 148: 173-182. https://doi.org/10.1016/j.lfs.2016.02.035
  6. Chung TY, Eiserich JP, Shibamoto T. Volatile compounds isolated from edible korean chamchwi (aster scaber thunb). J Agric Food Chem.1993. 41: 1693-1697. https://doi.org/10.1021/jf00034a033
  7. Cui Y, Zhang ZF, Froines J, Zhao J, Wang H, Yu SZ, Detels R. Air pollution and case fatality of sars in the people's republic of china: An ecologic study. Environ Health. 2003. 2: 15. https://doi.org/10.1186/1476-069X-2-15
  8. Dinarello CA. Proinflammatory cytokines. Chest. 2000. 118: 503-508. https://doi.org/10.1378/chest.118.2.503
  9. Halliwell B. Role of free radicals in the neurodegenerative diseases: Therapeutic implications for antioxidant treatment. Drugs Aging. 2001. 18: 685-716. https://doi.org/10.2165/00002512-200118090-00004
  10. Ignarro LJ. Physiology and pathophysiology of nitric oxide. Kidney Int Suppl. 1996. 55: S2-S5.
  11. Jeon SM, Lee JY, Kim HW, Lee YM, Jang HH, Hwang KA, Kim HR, Park DS. Antioxidant activity of extracts and fractions from aster scaber. J Korean Soc Food Sci Nutr. 2012. 41: 1197-1204. https://doi.org/10.3746/jkfn.2012.41.9.1197
  12. Jeong GS, Li B, Lee DS, Kim KH, Lee IK, Lee KR, Kim YC. Cytoprotective and anti-inflammatory effects of spinasterol via the induction of heme oxygenase-1 in murine hippocampal and microglial cell lines. Int Immunopharmacol. 2010. 10: 1587-1594. https://doi.org/10.1016/j.intimp.2010.09.013
  13. Karan A, Ali K, Teelucksingh S, Sakhamuri S. The impact of air pollution on the incidence and mortality of covid-19. Glob Health Res Policy. 2020. 5: 39. https://doi.org/10.1186/s41256-020-00167-y
  14. Kim EH, Lee S, Chung MJ. Anti-inflammatory and neuroprotective effects of astragalin isolated from aster scaber. Korean J Food Preserv. 2021. 28: 1000-1009. https://doi.org/10.11002/kjfp.2021.28.7.1000
  15. Kim EH, Shim YY, Lee HI, Lee S, Reaney MJT, Chung MJ. Astragalin and isoquercitrin isolated from aster scaber suppress lps-induced neuroinflammatory responses in microglia and mice. Foods. 2022. 11: 1505. https://doi.org/10.3390/foods11101505
  16. Kim GM, Hong JY, Woo SY, Lee HS, Choi YJ, Shin SR. Antioxidant activities of ethanol extracts of aster scaber grown in wild and culture field. Korean J Food Preserv. 2015. 22: 567-576. https://doi.org/10.11002/kjfp.2015.22.4.567
  17. Kim GH, Choi HY, Park HS. Antioxidant activity of aster scaber thunb. With different cooking methods (LB409). FASEB J. 2014. 28.
  18. Kim J, Park CS, Lim Y, Kim HS. Paeonia japonica, houttuynia cordata, and aster scaber water extracts induce nitric oxide and cytokine production by lipopolysaccharide-activated macrophages. J Med Food. 2009. 12: 365-373. https://doi.org/10.1089/jmf.2008.1013
  19. Kwon HC, Jung CM, Shin CG, Lee JK, Choi SU, Kim SY, Lee KR. Dictionary of Chinese herbs 1431. Bulletin P. 2000a. 48: 1796-1798.
  20. Kwon HC, Jung CM, Shin CG, Lee JK, Choi SU, Kim SY, Lee KR. A new caffeoly quinic acid from aster scaber and its inhibitory activity against human immunodeficiency virus-1 (hiv-1) integrase. Bulletin P. 2000b. 48: 1796-1798.
  21. Lee HI, Kim EH, Lee S, Chung MJ. Anti-inflammatory and neuroprotective effects of isoquercitrin isolated from aster scaber. J Korean Soc Food Sci Nutr. 2019. 48: 1205-1213. https://doi.org/10.3746/jkfn.2019.48.11.1205
  22. Lee SE, Lee JH, Kim JK, Kim GS, Kim YO, Soe JS, Choi JH, Lee ES, Noh HJ, Kim SY. Anti-inflammatory activity of medicinal plant extracts. Korean J Crop Sci. 2011. 19: 217-226. https://doi.org/10.7783/KJMCS.2011.19.4.217
  23. Lee TB. Illustrated flora of Korea. HMS. 1989.
  24. Lee YJ, Kim DB, Lee JS, Cho JH, Kim BK, Choi HS, Lee BY, Lee OH. Antioxidant activity and anti-adipogenic effects of wild herbs mainly cultivated in Korea. Molecules. 2013. 18: 12937-12950. https://doi.org/10.3390/molecules181012937
  25. Lee YY, Saba E, Kim M, Rhee MH, Kim HK. Antioxidant and anti-inflammatory properties of raw and processed fruits and vegetables. Biomed Sci Lett. 2018. 24: 196-205. https://doi.org/10.15616/BSL.2018.24.3.196
  26. Pizzino G, Irrera N, Cucinotta M, Pallio G, Mannino F, Arcoraci V, Squadrito F, Altavilla D, Bitto A. Oxidative stress: Harms and benefits for human health. Oxid Med Cell Longev. 2017. 2017: 8416763.
  27. Sahlmann CO, Strobel P. Pathophysiology of inflammation. Nuklearmedizin. 2016. 55: 1-6. https://doi.org/10.1055/s-0037-1616468
  28. Takeda K, Akira S. Toll-like receptors. Curr Protoc Immunol. 2015. 109: 14.12. 1-14.12. 10.
  29. Thiruvengadam M, Praveen N, Yu BR, Kim SH, Chung IM. Polyphenol composition and antioxidant capacity from different extracts of aster scaber. Acta Biol Hung. 2014. 65: 144-155. https://doi.org/10.1556/ABiol.65.2014.2.3
  30. Vazquez-Fresno R, Rosana ARR, Sajed T, Onookome-Okome T, Wishart NA, Wishart DS. Herbs and spices-biomarkers of intake based on human intervention studies - a systematic review. Genes Nutr. 2019. 14: 18. https://doi.org/10.1186/s12263-019-0636-8
  31. Whiteside M, Herndon JM. Covid-19 immunopathology, particle pollution, and iron balance. J Adv Med Med Res. 2020. 32: 43-60. https://doi.org/10.9734/jammr/2020/v32i1830654