Biomedical Science Letters (대한의생명과학회지)

The Korean Society for Biomedical Laboratory Sciences (대한의생명과학회)
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Comparison of CXCL10 Secretion in Colorectal Cancer Cell Lines

  • Lee, Song Mi (Department of Veterinary Medicine, College of Veterinary Medicine, Kyungpook National University) ;
  • Lee, Ji Eun (Department of Veterinary Medicine, College of Veterinary Medicine, Kyungpook National University) ;
  • Ahn, Hye Rim (Department of Veterinary Medicine, College of Veterinary Medicine, Kyungpook National University) ;
  • Choi, Myung Hyun (Department of Veterinary Medicine, College of Veterinary Medicine, Kyungpook National University) ;
  • Yoon, Seo Young (Department of Veterinary Medicine, College of Veterinary Medicine, Kyungpook National University) ;
  • Rhee, Man Hee (Department of Veterinary Medicine, College of Veterinary Medicine, Kyungpook National University) ;
  • Baik, Ji Sue (Research Center, Dongnam Institute of Radiological & Medical Sciences) ;
  • Seo, You Na (Research Center, Dongnam Institute of Radiological & Medical Sciences) ;
  • Park, Moon-Taek (Research Center, Dongnam Institute of Radiological & Medical Sciences) ;
  • Kim, Sung Dae (Department of Veterinary Medicine, College of Veterinary Medicine, Kyungpook National University)
  • Received : 2022.08.24
  • Accepted : 2022.09.22
  • Published : 2022.09.30
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Abstract

Established cancer cell lines are widely used for developing biomarkers for the patient-specific treatment of colorectal cancer and predicting prognoses. However, cancer cell lines may exhibit different drug responses depending upon the characteristics of the cell line. Therefore, it is necessary to select a tumor cell line suitable for the purpose of the study by considering the cell characteristics. This study investigated the levels of CXCL10, which were recently been reported to play an important role in the outcome of tumor treatment, secreted by colon cancer cells. 2 × 105 cells/mL of each colorectal cancer cell was seeded into a 35 mm cell culture dish. After 24 h incubation, culture supernatant was used to determine the secreted CXCL10 levels. Among six colorectal cancer cell lines (HT-29, HCT116, CaCo-2, SW620, SW480, and CT26), Caco-2 cells showed the highest level of CXCL10 secretion. HT-29 cells showed the second-highest level of CXCL10 secretion. No significantly measurable level of CXCL10 secretion was detected in HCT116 cells. These results will be helpful in investigating the molecular basis of colorectal cancer.

Keywords

Acknowledgement

This work was supported by a National Research Foundation of Korea grant funded by the Korean government (MSIP) (2022R1A2C1011767 and DIRAMS 50591-2022).

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