DOI QR코드

DOI QR Code

Determination of Amino Acid Composition in Leaf, Stem, and Inflorescence of Alfalfa (Medicago sativa L.)

  • Muthusamy, Karnan (Grassland and Forages Division, National Institute of Animal Science, Rural Development Administration) ;
  • Ilavenil, Soundharrajan (Grassland and Forages Division, National Institute of Animal Science, Rural Development Administration) ;
  • Jung, Jeong Sung (Grassland and Forages Division, National Institute of Animal Science, Rural Development Administration) ;
  • Lee, Bae Hun (Grassland and Forages Division, National Institute of Animal Science, Rural Development Administration) ;
  • Nam, Cheol Hwan (Livestock Institute, Jeollanamdo Agricultural Research and Extension Services) ;
  • Park, Hyung Soo (Grassland and Forages Division, National Institute of Animal Science, Rural Development Administration) ;
  • Choi, Ki Choon (Grassland and Forages Division, National Institute of Animal Science, Rural Development Administration)
  • Received : 2021.09.02
  • Accepted : 2022.09.26
  • Published : 2022.09.30

Abstract

Alfalfa is one of the most useful forage crops worldwide, containing a high level of amino acids that are essential to both human and animal health. However, amino acids and their concentrations may differ between plant parts. Hence, detecting amino acids in different plant parts would be useful in the development of diet supplements. The purpose of this study was to determine the amino acid content in alfalfa leaves, stems, and inflorescences using an amino acid analyzer. Asparagine and glutamic acid were the most abundant amino acids found in stems, leaves, and inflorescences than other amino acids. All parts of alfalfa had low concentrations of cysteine and methionine. All amino acids except asparagine were present in the highest concentration in leaves followed by inflorescences. Leaf had a rich amino acid content, namely asparagine, glutamic acid, leucine, proline, and lysine. However, the stem had a lower amino acid composition than the leaf or inflorescence. Overall, the data showed determining the amino acid content of forages provides a good approach to making animal feed with essential and specific amino acids and preventing excessive inclusion of amino acids.

Keywords

Acknowledgement

Cooperative Research Program for Agriculture Science and Technology Development supported funds for this research work (Project No. PJ01358902). The project titled "technique development for the manufacture of high-quality legume silage "sponsored by RDA, Korea. This study was also supported by the Postdoctoral Fellowship Program of the National Institute of Animal Science funded by RDA, Korea.

References

  1. Apostol, L., Iorga, S., Mosoiu, C., Racovita, R., Niculae, O. and Vlasceanu, G. 2017. Alfalfa concentrate-A rich source of nutrients for use in food products. Agriculture and Food. 5:66-73.
  2. Arinze, E.A., Schoenau, G.J., Sokhansanj, S. and Adapa, P. 2003. Aerodynamic separation and fractional drying of alfalfa leaves and stems-A review and new concept. Drying Technology. 21(9): 1669-1698. https://doi.org/10.1081/DRT-120025503
  3. Arlabosse, P. and Blanc, M. 2011. Thermo-mechanical fractionation of green biomass: Optimisation of the processing conditions according to the water potential of the tissue, EuroDrying'2011-III European drying conference. Universitat de les illes balears, Palma de Mallorca, Spain, p. 3 p.Bora, K., Sharma. B 8, 4.
  4. Blume, L., Hoischen-Taubner, S. and Sundrum, A. 2021. Alfalfa-A regional protein source for all farm animals. Journal of Sustainable and Organic Agricultural Systems. 71(1):1-13.
  5. Chiesa, S. and Gnansounou, E. 2011. Protein extraction from biomass in a bioethanol refinery-Possible dietary applications: Use as animal feed and potential extension to human consumption. Bioresource Technology. 102(2):427-436. https://doi.org/10.1016/j.biortech.2010.07.125
  6. Choi, K.C., Ryu, J.H., Jung, M.W., Park, H.S., Kim, C.M., Lim, Y.C. and Kim, W.H. 2012. Changes of amino acid contents in white panicled rice (Oryza sativa L.). Journal of the Korean Society of Grassland and Forage Science. 32(2):93-100. https://doi.org/10.5333/KGFS.2012.32.2.93
  7. Giner-Chavez, B., Soest, P., Robertson, J., Lascano, C. and Pell, A. 1997. Comparison of the precipitation of alfalfa leaf protein and bovine serum albumin by tannins in the radial diffusion method. Journal of the Science of Food and Agriculture. 74(4):513-523. https://doi.org/10.1002/(SICI)1097-0010(199708)74:4<513::AID-JSFA825>3.0.CO;2-B
  8. Guo, Z., Cao, W. and Liao, H. 2007. Review on the research progress in leaf protein. J Suzhou University. 47:124-132.
  9. Hojilla-Evangelista, M.P., Selling, G.W., Hatfield, R. and Digman, M. 2017. Extraction, composition, and functional properties of dried alfalfa (Medicago sativa L.) leaf protein. Journal of the Science of Food and Science Agriculture. 97(3):882-888. https://doi.org/10.1002/jsfa.7810
  10. Hsu, A. and Allee, G. 1980. Limiting order of amino acid in AlfalfaLeaf Protein Concentrate (ALPC) for growing pigs. Kansas Agricultural Experiment Station Research Reports. 10:6-9. https://doi.org/10.4148/2378-5977.6066
  11. Lyle Livingston, A., Allis, M.E. and Kohler, G.O. 1971. Amino acid stability during alfalfa dehydration. Journal of Agricultural and Food Chemistry. 19(5):947. https://doi.org/10.1021/jf60177a023
  12. Manjarin, R., Bequette, B.J., Wu, G. and Trottier, N.L. 2014. Linking our understanding of mammary gland metabolism to amino acid nutrition. Amino Acids. 46(11):2447-2462. https://doi.org/10.1007/s00726-014-1818-8
  13. Patton, R.A., Hristov, A.N. and Lapierre, H. 2014. Protein feeding and balancing for amino acids in lactating dairy cattle. The Veterinary clinics of North America. Food Animal Practice. 30(3):599-621. https://doi.org/10.1016/j.cvfa.2014.07.005
  14. Radovic, J., Sokolovic, D. and Markovic, J. 2009. Alfalfa-most important perennial forage legume in animal husbandry. Biotechnology in Animal Husbandry. 25(5-6):465-475. https://doi.org/10.2298/BAH0906465R
  15. Sadeghi, L., Tanwir, F. and Yousefi Babadi, V. 2016. Antioxidant effects of alfalfa can improve iron oxide nanoparticle damage: Invivo and invitro studies. Regulatory Toxicology and Pharmacology. 81:39-46. https://doi.org/10.1016/j.yrtph.2016.07.010
  16. Sahni, P., Sharma, S. and Surasani, V.K.R. 2020. Influence of processing and pH on amino acid profile, morphology, electrophoretic pattern, bioactive potential and functional characteristics of alfalfa protein isolates. Food Chemistry. 333:127503. https://doi.org/10.1016/j.foodchem.2020.127503
  17. Van Krimpen, M., Bikker, P., Van der Meer, I.M., Van Der Peet-Schweing, C.M.C. and Vereijken, J.M. 2013. Cultivation, processing and nutritional aspects for pigs and poultry of European protein sources as alternatives for imported soybean products. Report, 662.
  18. Wustholz, J., Carrasco, S., Berger, U., Sundrum, A. and Bellof, G. 2017. Silage of young harvested alfalfa (Medicago sativa) as home-grown protein feed in the organic feeding of laying hens. Organic Agriculture. 7:153-163. https://doi.org/10.1007/s13165-016-0151-9
  19. Zhang, W., Grimi, N., Jaffrin, M., Ding, L.H. and Tang, B. 2017. A short review on the research progress in alfalfa leaf protein separation technology. Journal of Chemical Technology and Biotechnology. 92(12):2894-2900. https://doi.org/10.1002/jctb.5364