Natural Product Sciences

  • 제28권3호
  • /
  • Pages.130-137
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  • 2022
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  • 1226-3907(pISSN)
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  • 2288-9027(eISSN)
한국생약학회 (The Korean Society of Pharmacognosy)
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DOI QR코드

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Phytochemical Characterization of Vitex negundo Leaves: a Potent Antiandrogenic and Antioxidant Agent

  • 투고 : 2022.04.07
  • 심사 : 2022.07.29
  • 발행 : 2022.09.30
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초록

This study was conducted to characterise phytochemicals and to explore the biological activities of Vitex negundo leaves. The washed, course powder of V. negundo leaves were extracted with different solvents of increasing polarity. All the extracts were characterized and biological activities were compared. The results revealed that the ethanolic and cold water extracts showed the presence of all phytochemicals studied except protein compared to other extracts. Further, the quantitative estimation of phytochemicals showed that the ethanolic extract had highest yield and maximum amount of total polyphenols, flavonoids, and alkaloids with the least amount of tannins compared to other extracts studied. Furthermore, the highest total polyphenol content corresponds with the potent biological activities. Indeed, in vitro antioxidant and antisteroidogenic activities were highest in the ethanolic extract than others. To conclude, the present study is the first to report the characterization and antiandrogenic property of V. negundo leaf extracts. The ethanolic extract of V. negundo leaves can be used as an antioxidant and antiandrogenic agent. Hence, it can be considered for the treatment of hyperandrogenic conditions like polycystic ovary syndrome, etc.

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과제정보

We thank University of Mysore for the award of fellowship to the first author University SC/ST special cell fellowship scheme. We would also thank University of Mysore for the equipment facility provided in the Institution of Excellence.

참고문헌

  1. Mazid, M.; Khan, T. A.; Mohammad, F. Biol. Med. 2011, 3, 232-249.
  2. Pandey, K. B.; Rizvi, S. I. Oxid. Med. Cell. Longev. 2009, 2, 270-278. https://doi.org/10.4161/oxim.2.5.9498
  3. Keerti, G.; Padma, K. Int. J. Drug Dev. Res. 2012, 4, 192-199.
  4. Bano, U.; Jabeen, Z.; Ahmed, A.; Siddiqui, M. A. World J. Pharm. Res. 2015, 4, 589-606.
  5. Warrier, P. K.; Nambiar, V. P. K. Indian medicinal plants: A compendium of 500 species. Vol. 5; Orient Longman Private Limited: India, 2002, p 387.
  6. Chaturvedi, G. N.; Singh, R. H. Indian J. Med. Res. 1965, 53, 71-80.
  7. Chawla, A. S.; Sharma, A. K.; Handa, S. S.; Dhar, K. L. Indian J. Chem. 1991, 30B, 773-776.
  8. Chawla, A. S.; Sharma, A. K.; Handa, S. S.; Dhar, K. L. J. Nat. Prod. 1992, 55, 163-167. https://doi.org/10.1021/np50080a002
  9. Utpalendu, J.; Chattopadhyay, R. N.; Shaw, B. P. Indian J. Pharmacol. 1999, 3, 232-233.
  10. Kulkarni, R. R.; Virkar, A. D.; D'mello, P. Indian J. Pharm. Sci. 2008, 70, 838-840.
  11. Singh, D. D.; Chitra, G.; Singh, I. P.; Bhutani, K. K. Indian J. Chem. 2005, 44B, 1288-1290.
  12. Bhargava, S. K. J. Ethnopharmacol. 1989, 27, 327-339. https://doi.org/10.1016/0378-8741(89)90007-X
  13. Samy, P. R.; Ignacimuthu, S.; Sen, A. J. Ethnopharmacol. 1998, 62, 173-182. https://doi.org/10.1016/S0378-8741(98)00057-9
  14. Deshmukh, P. B.; Chavan, S. R.; Renapurkar, D. M. Pesticides, 1982, 16, 7-10.
  15. Hebbalkar, D. S.; Hebbalkar, G. D.; Sharma, R. N.; Joshi, V. S.; Bhat, V. S. Indian J. Med. Res. 1992, 95, 200-203.
  16. Alam, M. I.; Gomes, A. J. Ethnopharmacol. 2003, 86, 75-80. https://doi.org/10.1016/S0378-8741(03)00049-7
  17. Zheng, G.; Luo, Z. Guangdong Huangong, 1999, 2, 8-9.
  18. Ono, M.; Nishida, Y.; Masuoka, C.; Li, J.; Okawa, M.; Ikeda, T.; Nohara, T. J. Nat. Prod. 2004, 67, 2073-2075. https://doi.org/10.1021/np040102t
  19. Dharmasiri, M. G.; Jayakody, J. R. A. C.; Galhena, G.; Liyanage, S. S. P.; Ratnasooriyab, W. D. J. Ethnopharmacol. 2003, 87, 199-206. https://doi.org/10.1016/S0378-8741(03)00159-4
  20. Kadir, F. A.; Kassim, N. M.; Abdulla, M. A.; Yehye, W. A. Evid. Based Complement Alternat. Med. 2013, 2013, 739850.
  21. Tondon, V. R.; Gupta, R. K. Indian J. Physiol. Pharmacol. 2005, 49, 199-205.
  22. Tiwari, O. P.; Tripathi, Y. B. Food Chem. 2007, 100, 1170-1176. https://doi.org/10.1016/j.foodchem.2005.10.069
  23. Jivarajani, M.; Ravat, N.; Anandjiwala, S.; Nivsarkar, M. Int. Sch. Res. Notices 2014, 2014, 241946.
  24. Kannan, M.; Rajendran, P.; Vedha, V.; Ashok, G.; Anushka, S.; Ramachandran Nair, P. C. J. Cell Mol. Biol. 2012, 10, 53-59.
  25. Mumtaz, F.; Raza, S. M.; Ahmad, Z.; Iftikhar, A.; Hussain, M. J. Pharm. Altern. Med. 2014, 3, 17-21.
  26. McDonald, S.; Prenzler, P. D.; Antolovich, M.; Robards, K. Food Chem. 2001, 73, 73-84. https://doi.org/10.1016/S0308-8146(00)00288-0
  27. Chang, C. C.; Yang, M. H.; Wen, H. M.; Chern, J. C. J. Food Drug Anal. 2002, 10, 178-182.
  28. Herald, T. J.; Gadgil, P.; Perumal, R.; Bean, S. R.; Wilson, J. D. J. Sci. Food Agric. 2014, 94, 2133-2136. https://doi.org/10.1002/jsfa.6538
  29. Fadhil, S.; Reza, M. H.; Rouhollah, G.; Reza, V. R. M. Res. J. Phytochem. 2007, 1, 79-82. https://doi.org/10.3923/rjphyto.2007.79.82
  30. Rakholiya, K.; Kaneria, M.; Chanda, S. J. Med. Plants Res. 2011, 5, 63-71.
  31. Shivanandappa, T.; Venkatesh, S. Anal. Biochem. 1997, 254, 57-61.
  32. Pagare, S.; Bhatia, M.; Tripathi, N.; Pagare, S.; Bansal, Y. K. Curr. Trends Biotechnol. Pharm. 2015, 9, 293-304.
  33. Farnsworth, N. R.; Akerele, O.; Bingel, A. S.; Soejarto, D. D.; Guo, Z. Bull. World Health Organ. 1985, 63, 965-981.
  34. Ramadan, G.; Al-Kahtani, M. A.; El-Sayed, W. M. Inflammation 2011, 34, 291-301.
  35. Savaringal, J. P.; Lally, M. S. Int. J. Basic Clin. Pharmacol. 2018, 7, 229-233. https://doi.org/10.18203/2319-2003.ijbcp20180032
  36. Juarez-Rijop, I. E.; Diaz-Zagoya, J. C.; Ble-Castillo, J. L.; MirandaOscorio, P. H.; Castell-Rodriguez, A. E.; Tovilla-Zarate, C. A.; RodriguezHernandez, A.; Aguilar-Mariscal, H.; Ramon-Fris, T.; Bermudez-Ocana, D. Y. BMC Complement. Altern. Med. 2012, 12, 236.
  37. Sharma, N.; Bano, A.; Dhaliwal, H. S.; Sharma, V. Int. J. Pharm. Pharm. Sci. 2015, 7, 30-34.
  38. Zarei, M.; Javarappa, K. K.; Zarei, M.; Baker, S. Der Pharmacia Lettre 2013, 5, 334-339.
  39. Kim, D. S.; Kim, S. H.; Cha, J. Evid. Based Complement. Altern. Med. 2016, 2016, 9735276.
  40. Jeyachandran, R.; Xavier, T. F.; Anand, S. P. Anc. Sci. Life 2003, 23, 40-43
  41. Gul, P.; Bakht, J. J. Food Sci. Technol. 2015, 52, 2272-2279.
  42. Sarjan, H. N.; Divyashree, S.; Yajurvedi, H. N. Pharm. Biol. 2017, 55, 1358-1367.
  43. Ahirrao, R. A.; Patel, M. R. Asian J. Res. Chem. 2012, 5, 843-845.
  44. Zheng, C. J.; Huang, B. K.; Han, T.; Zhang, Q. Y.; Zhang, H.; Rahman, K.; Qin, L. P. Pharma. Biol. 2010, 48, 651-658.
  45. Zhao, X. Y.; Wang, B. E.; Li, X. M.; Wang, T. L. Pathol. Int. 2008, 58, 580-588.
  46. Alshawsh, M. A.; Abdulla, M. A.; Ismail, S.; Amin, Z. A. Evid. Based Complement. Alternat Med. 2011, 2011, 103039.
  47. Mahalakshmi, R.; Rajesh, P.; Ramesh, N.; Balasubramanian, V.; Kannan, V. R. Int. J. Pharmacol. 2010, 6, 658-663. https://doi.org/10.3923/ijp.2010.658.663
  48. Dashti, H. M.; Mathew, T. C.; Jadaon, M. M.; Ashkanani, E. Nutrition 1997, 13, 206-212. https://doi.org/10.1016/S0899-9007(96)00403-0
  49. Nakajima, M.; Iwata, K.; Yamamoto, T.; Funae, Y.; Yoshida, T.; Kuroiwa, Y. Drug Metab. Dispos. 1998, 26, 36-41.
  50. Prakash, A.; Mathur, K. C. Bull. Grain Technol. 1985, 23, 278-281.
  51. Ravishankar, B.; Bhaskaran, N. R.; Sasikala, C. K. Bull. MedicoEthno-Botanical Res. 1985, 6, 72-92.
  52. Ravishankar, B.; Bhaskaran, N. R.; Sasikala, C. K. J. Res. Ayurv. Siddha. 1986, 7, 62-77.
  53. Kurapatti, P.; Murugesan, K.; Anbalagan, S.; Sankareswaran, M. Int. J. Pharm. Sci. Rev. Res. 2017, 46, 183-187.
  54. Ueda, K.; Kawabata, R.; Irie, T.; Nakai, Y.; Tohya, Y.; Sakaguchi, T. PLoS ONE, 2013, 8, e55343. https://doi.org/10.1371/journal.pone.0055343
  55. Ashok, P. K.; Upadhyaya, K. J. Pharmacogn. Phytochem. 2012, 1, 45-50.
  56. Sumathy, R.; Sankaranarayanan, S.; Bama, P.; Ramachandram, J.; Vijayalakshmi, M.; Deecaraman, M. Asian J. Pharm. Clin. Res. 2013, 6, 211-214.
  57. Scalbert, A.; Johnson, I. T.; Saltmarsh, M. Am. J. Clin. Nutr. 2005, 81, 215S-217S. https://doi.org/10.1093/ajcn/81.1.215S
  58. Lakshmanashetty, R. H.; Nagaraj, V. B.; Hiremath, M. G.; Kumar, V. Chiang Mai J. Sci. 2010, 37, 489-497.
  59. Zargar, M.; Azizah, A. H.; Roheeyati, A. M.; Fatimah, A. B.; Jahanshiri, F.; Pak-Dek, M. S. J. Med. Plants Res. 2011, 5, 2525-2532.
  60. Divya, K.S.; Swati, S.P. Int. J. Pharm. Sci. Rev. Res. 2015, 33, 211-216.