The Korea Journal of Herbology (대한본초학회지)

The Korea Association of Herbology (대한본초학회)
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Analysis of Components in the Different Parts of Lythrum salicaria L.

털부처꽃의 부위별 성분 분석

  • Kim, Hee-Young (Department of Pharmacology, College of Korean Medicine, Sangji University) ;
  • Park, Yea-Jin (Department of Pharmacology, College of Korean Medicine, Sangji University) ;
  • Lee, Ju-Yeon (Department of Pharmacology, College of Korean Medicine, Sangji University) ;
  • Kim, Ki-young (Research Institute, Bio Port Korea Inc.) ;
  • Shin, Su (Research Institute, Bio Port Korea Inc.) ;
  • Choi, Min-Woo (Phyheal Inc.) ;
  • Hong, Eun-Jin (Research Institute, Bio Port Korea Inc.) ;
  • Kim, Min-jeong (Department of Acupuncture and Moxibustion Medicine, College of Korean Medicine, Sangji University) ;
  • Yeo, Sujung (Department of Meridian & Acupoint, College of Korean Medicine, Sangji University) ;
  • Park, In-hwa (Department of Rehabilitation Medicine of Korean Medicine, College of Korean Medicine, Sangji University) ;
  • Jerng, Ui Min (Department of Internal Medicine, College of Korean Medicine, Sangji University) ;
  • An, Hyo-Jin (Department of Pharmacology, College of Korean Medicine, Sangji University) ;
  • Cha, Yun-Yeop (Department of Rehabilitation Medicine of Korean Medicine, College of Korean Medicine, Sangji University)
  • 김희영 (상지대학교 한의과대학 약리학교실) ;
  • 박예진 (상지대학교 한의과대학 약리학교실) ;
  • 이주연 (상지대학교 한의과대학 약리학교실) ;
  • 김기영 ((주)바이오포트코리아 기업부설연구소) ;
  • 신수 ((주)바이오포트코리아 기업부설연구소) ;
  • 최민우 ((주)파이힐) ;
  • 홍은진 ((주)바이오포트코리아 기업부설연구소) ;
  • 김민정 (상지대학교 한의과대학 침구의학교실) ;
  • 여수정 (상지대학교 한의과대학 경혈학교실) ;
  • 박인화 (상지대학교 한의과대학 한의학과 재활의학과) ;
  • 정의민 (상지대학교 한의과대학 내과) ;
  • 안효진 (상지대학교 한의과대학 약리학교실) ;
  • 차윤엽 (상지대학교 한의과대학 한의학과 재활의학과)
  • Received : 2022.09.06
  • Accepted : 2022.09.25
  • Published : 2022.09.30
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Abstract

Objectives : This research was performed to analyze the components in the different parts of Lythrum salicaria L. and to compare which parts of L. salicaria L. are appropriate for food development. Methods : L. salicaria L. was extracted in 20% EtOH at 100 ℃ for 4 hours. Cytotoxicity was investigated in 3T3-L1 cells after treatment of 10-500 ㎍/ml L. salicaria L. for 24 hours. Total polyphenol content (TPC) was estimated using 1 N Folin-ciocateu reagent. 2,2-Diphenyl-1-picryhydrazyl (DPPH) radical scavenging activity was estimated using DPPH reagent and gallic acid. The chemical composition was analyzed by high-performance liquid chromatography (HPLC). 1) Results : The half maximal inhibitory concentration (IC50) in the extracts of the whole plant, aerial parts, and root parts was 350 ㎍/ml, over 500 ㎍/ml, and 150 ㎍/ml, respectively. The TPC in the extracts of the whole plant, aerial parts, and root parts was 527.1 mg/g, 422.6 mg/g, and 781.1 mg/g, respectively. The averages of vitexin contents in the aerial parts, and root parts were 256.7 ± 154.9 ㎍/g and 266.1 ± 63.2 ㎍/g, respectively. The averages of TPC in the leaves, roots, flower stalks and stems were 224.0 ± 53.7 tannin acid (TA) mg/g, 221.8 ± 70.2 TA mg/g, 249.8 ± 34.4 TA mg/g, and 67.7±8.9 TA mg/g, respectively. The averages of DPPH radical scavenging activity in the leaves, roots, flower stalks, and stems were 282.01 ± 43.3 gallic acid equivalent (GAE) 𝜇mole/g, 260.16 ± 44.1 GAE 𝜇mole/g, 288.0 ± 9.3 GAE 𝜇mole/g, and 97.6 ± 10.7 GAE 𝜇mole/g, respectively. Conclusions : There were no significant differences in the content of components or antioxidant activity in the aerial parts compared to those in the whole plant of L. salicaria L. Furthermore, the root parts had low extract yield, cytotoxicity, and quality control problems, therefore our results suggest that the use of the aerial part of L. salicaria L. would be the most appropriate for food development.

Keywords

Acknowledgement

이 연구는 농촌진흥청 연구사업(세부과제번호: PJ016132012022)과 2020년 상지대학교 교내과제 지원사업 및 상지대학교 대학원 지원에 의하여 수행되었음

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