The Korea Journal of Herbology (대한본초학회지)

The Korea Association of Herbology (대한본초학회)
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A Case Report of Imports Morphological Variation of Pinelliae Tuber Based on the Genetic Analysis

유전자 분석 기반 수입산 형태 변이 반하 유통 사례 보고

  • 김욱진 (한국한의학연구원 한약자원연구센터) ;
  • 최고야 (한국한의학연구원 한약자원연구센터) ;
  • 노수민 (한국한의학연구원 한약자원연구센터) ;
  • 문병철 (한국한의학연구원 한약자원연구센터)
  • Received : 2022.06.15
  • Accepted : 2022.09.25
  • Published : 2022.09.30
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Abstract

Objectives : The purpose of this study is to report that applying the genetic discrimination method to Pinelliae Tuber is suitable as a countermeasure for the limitations of morphological identification announced publicly in the Ministry of Food and Drug Safety(MFDS). Methods : Randomly selected fifty samples in Pinelliae Tuber imported from China were used for morphological and genetic identification. The morphological identification was applied method announced publicly by the MFDS. The traits of morphological identification were classified as Pinellia ternata, P. tripartita, Pinellia pedatisecta, and Typhonium flagelliforme, according to the formation of tuberous root and tuber morphology. The genetic identifications were conducted by Sequence Characterized Amplified Region(SCAR) marker and DNA barcoding analysis for cross-validation, respectively. SCAR marker was verified according to the presence or absence of amplicon through PCR amplification using species-specific primers. DNA barcoding analysis used sequence information of the matK region. Results : As a result of the morphological identification, 27 out of 50 samples were identified as original species 'P. ternata' of genuine 'Pinelliae Tuber', and 23 were identified as adulterant species 'P. pedatisecta'. Unlike this, the genetic identification was identified as the original species 'P. ternata' in all 50 samples in the SCAR marker and matK regional sequence analysis. Conclusions : Pinelliae Tuber of morphological mutant that can not be classified by morphological identification is imported from China. The SCAR marker would be used as accurate and efficient assays for species identification of the morphological mutant.

Keywords

Acknowledgement

이 논문은 2022년도 과학기술정보통신부 재원으로 연구개발특구진흥재단에서 지원하는 '마이크로바이옴 기반 스마트 웰에이징 기술 개발'(No.2021-DD-UP-0380) 및 한국한의학연구원의 '지속가능한 한약표준자원 활용기술 개발'(KSN 2013320) 과제의 지원을 받아 수행된 연구입니다. 한국한의학연구원 한약표준표본관 및 시료의 분류·동정에 도움을 주신 분류·동정자문위원님께 감사드립니다.

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