The Korean Journal of Pain

The Korean Pain Society (대한통증학회)
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Stem cells from human exfoliated deciduous teeth attenuate trigeminal neuralgia in rats by inhibiting endoplasmic reticulum stress

  • Yang, Zhijie (Department of Oral and Maxillofacial Surgery, Hospital of Stomatology, China Medical University) ;
  • Wang, Chun (Department of Anesthesia, First Affiliated Hospital, Zhejiang University School of Medicine) ;
  • Zhang, Xia (Liaoning Provincial Key Laboratory of Oral Diseases) ;
  • Li, Jing (Department of Oral and Maxillofacial Surgery, Hospital of Stomatology, China Medical University) ;
  • Zhang, Ziqi (Department of Oral and Maxillofacial Surgery, Hospital of Stomatology, China Medical University) ;
  • Tan, Zhao (Department of Oral and Maxillofacial Surgery, Hospital of Stomatology, China Medical University) ;
  • Wang, Junyi (Department of Oral and Maxillofacial Surgery, Hospital of Stomatology, China Medical University) ;
  • Zhang, Junyang (Department of Oral and Maxillofacial Surgery, Hospital of Stomatology, China Medical University) ;
  • Bai, Xiaofeng (Department of Oral and Maxillofacial Surgery, Hospital of Stomatology, China Medical University)
  • Received : 2022.03.23
  • Accepted : 2022.06.13
  • Published : 2022.10.01
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Abstract

Background: The treatment of trigeminal neuralgia remains a challenging issue. Stem cells from human exfoliated deciduous teeth (SHED) provide optimized therapy for chronic pain. This study aimed to investigate the mechanisms underlying the attenuation of trigeminal neuralgia by SHED. Methods: Trigeminal neuralgia was induced by chronic constriction injury of the infraorbital nerve. The mechanical threshold was assessed after model establishment and local SHED transplantation. Endoplasmic reticulum (ER) morphology and Caspase12 expression in trigeminal ganglion (TG) was evaluated as well. BiP expression was observed in PC12 cells induced by tunicamycin. Results: The local transplantation of SHED could relieve trigeminal neuralgia in rats. Further, transmission electron microscopy revealed swelling of the ER in rats with trigeminal neuralgia. Moreover, SHED inhibited the tunicamycin-induced up-regulated expression of BiP mRNA and protein in vitro. Additionally, SHED decreased the up-regulated expression of Caspase12 mRNA and protein in the TG of rats caused by trigeminal neuralgia after chronic constriction injury of the infraorbital nerve mode. Conclusions: This findings demonstrated that SHED could alleviate pain by relieving ER stress which provide potential basic evidence for clinical pain treatment.

Keywords

Acknowledgement

We would like to thank central lab of China Medical University for assistance.

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