Korean Journal of Veterinary Service (한국동물위생학회지)

The Korean Society of Veterinary Service (한국동물위생학회)
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Monitoring for fluoroquinolones residues in raw meat in Sejong

세종지역 유통 식육의 플루오로퀴놀론계 항생제 잔류 연구

  • 정윤경 (세종특별자치시 보건환경연구원) ;
  • 이태호 (세종특별자치시 보건환경연구원) ;
  • 이종훈 (세종특별자치시 보건환경연구원) ;
  • 김문배 (세종특별자치시 보건환경연구원)
  • Received : 2022.06.23
  • Accepted : 2022.08.29
  • Published : 2022.09.30
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Abstract

This study describes an analytical method based on LC-MS/MS for the quantitation of 5 fluoroquinolones (Enrofloxacin, Ciprofloxacin, Marbofloxacin, Norfloxacin and Danofloxacin) in meat, and was applied to 230 meat samples for validation. Quantitation was performed based on a matrix-matched calibration to compensate for the matrix effect on the electrospray ionization. Good linear calibrations (R2≥0.998) were obtained for all fluoroquinolones at 6 concentrations of 1~50 ㎍/kg. Satisfied recoveries of all fluoroquinolones were demonstrated in spiked meat at three levels from 10 to 50 ㎍/kg. The recoveries ranged between 75.8~99.2% in beef, 80.1~99.6% in pork and 72.2~99.8% in chicken, respectively. The limits of quantitation (LOQs) for fluoroquinolones ranged from 0.7 to 3.2 ㎍/kg. We also monitored fluoroquinolones residue in the sample (beef 107, pork 71, chicken 52) using LC-MS/MS. Residues of fluoroquinolones which exceeded maximum residue limits (MRL) were not exceed in any of the 230 samples.

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References

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