Korean Journal of Veterinary Service (한국동물위생학회지)

The Korean Society of Veterinary Service (한국동물위생학회)
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Prevalence and characterization of pathogenic Escherichia coli from carcasses and environmental samples of cattle and pig slaughterhouses

소와 돼지 도축장 도체 및 환경에서 분리된 병원성대장균 분포 및 특성조사

  • Hong, Serim (Bacterial Disease Division, Animal and Plant Quarantine Agency) ;
  • Kang, Hye Jeong (Bacterial Disease Division, Animal and Plant Quarantine Agency) ;
  • Moon, Jin-San (Bacterial Disease Division, Animal and Plant Quarantine Agency) ;
  • Yoon, Soon-Seek (Bacterial Disease Division, Animal and Plant Quarantine Agency) ;
  • Kim, Ha-Young (Bacterial Disease Division, Animal and Plant Quarantine Agency)
  • 홍세림 (농림축산검역본부 세균질병과) ;
  • 강혜정 (농림축산검역본부 세균질병과) ;
  • 문진산 (농림축산검역본부 세균질병과) ;
  • 윤순식 (농림축산검역본부 세균질병과) ;
  • 김하영 (농림축산검역본부 세균질병과)
  • Received : 2022.08.05
  • Accepted : 2022.09.02
  • Published : 2022.09.30
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Abstract

We investigated the virulence genes, O-serotypes, antimicrobial resistance of pathogenic E. coli isolated from carcasses (n=455) and environmental (n=372) samples of 11 cattle and 12 pig slaughterhouses from December 2020 to December 2021. E. coli were isolated from nine carcasses (2.0%), three slaughter facilities (1.4%), two utensils (2.7%) and three abattoir workers (3.5%) from four cattle and four pig slaughterhouses. Among all isolates, 13 STEC (76.5%) were identified, followed by four EPEC (23.5%). As a result of the antibiotic susceptibility test, all isolates were resistant to at least one antimicrobial, of which 70.6% isolates showed multidrug resistance patterns. The serotypes were diverse in pigs compared to cattle, with serotypes O18, O66, O109 in cattle and O9, O76, O85, O100, O153, and O159 in pigs. In a single cattle slaughterhouse, eight STEC O66 were isolated from various types of sample (4 slaughter animal surfaces, 3 gloves, and 1 knife) with two antimicrobial resistance patterns (CHL-FIS-STR and CHL-FIS). Those two types of strain were suspected cross-contamination from utensils to slaughter animal surfaces. These results showed that pathogenic E. coli were detected in carcasses and various environmental samples in cattle and pig slaughterhouses. Nationwide monitoring and hygiene management are required to prevent cross-contamination of STEC isolate slaughterhouses.

Keywords

Acknowledgement

본 연구는 농림축산검역본부의 농림축산검역검사기술개발사업(과제명: 도축장 내 클로스트리듐과 예르시니아균 오염실태 및 특성조사, B-1543081-2022-23-02)의 연구비 지원에 의해 수행되었다.

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