Journal of Physiology & Pathology in Korean Medicine (동의생리병리학회지)

The Physiological Society of Korean Medicine and The Society of Pathology in Korean Medicine (대한동의생리학회·한의병리학회)
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In vitro Anti-oxidant and Anti-inflammatory Effects of Moringa Folium and Eucommiae Cortex 2:1 (g/g) Mixed Formula

모링가:두충 2:1 (g:g) 복합 조성물의 in vitro 항산화 및 항염 효능 연구

  • Heo, Seok-Mo (Department of Periodontology, School of Dentistry, Chonbuk National University) ;
  • Yang, Jin-Won (Department of Anatomy and Histology, College of Korean Medicine, Daegu Haany University) ;
  • Kim, Jong-Lae (HLscience Co., Ltd) ;
  • Park, Mi-Ryeong (HLscience Co., Ltd) ;
  • Kim, Tae Gi (HLscience Co., Ltd) ;
  • Ku, Sae-Kwang (Department of Anatomy and Histology, College of Korean Medicine, Daegu Haany University) ;
  • Park, Soo-Jin (Department of Physiology, College of Korean Medicine, Daegu Haany University)
  • 허석모 (전북대학교 치과대학 치주과학) ;
  • 양진원 (대구한의대학교 해부조직학교실) ;
  • 김종래 (에이치엘사이언츠) ;
  • 박미령 (에이치엘사이언츠) ;
  • 김태기 (에이치엘사이언츠) ;
  • 구세광 (대구한의대학교 해부조직학교실) ;
  • 박수진 (대구한의대학교 생리학교실)
  • Received : 2022.05.05
  • Accepted : 2022.08.24
  • Published : 2022.08.25
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Abstract

The purpose of study is to evaluate in vitro anti-oxidant and anti-inflammatory effects of Moringa Folium and Eucommiae Cortex 2:1 (g/g) mixtures (MEMix). HaCaT and human normal dermal fibroblast were treated with 0.01-1 mg/mL of MEMix to monitor cytotoxicity. Radical scavenging activities of MEMix were examined by DPPH assay. To explore anti-inflammatory effect, Raw 264.7 cells were pretreated with MEMix for 1h and subsequently exposed to LPS for 18h. NO release and cytotoxicity of Raw 264.7 cells were measured by adding Griess and MTT reagents, respectively. TNF-α, IL-1β, IL-6, and PGE2 productions were examined by ELISA. Immunoblot analysis was conducted to examine COX-2 expression in MEMix pretreated Raw 264.7 cells. Up to 1 mg/mL concentration, treatment of MEMix for 24 h did not affect normal dermal fibroblast viability and significantly reduced cell viability of HaCaT cells with no concentration dependency. MEMix increased DPPH radical scavenging activity with concentration dependency. Radical scavenging activities by 1 mg/mL of MEMix was comparable with 30 µM of trolox. Pretreatment of MEMix did not change the reduction of Raw 264.7 cell viability. Exposure of LPS in Raw 264.7 cells significantly increased NO, TNF-α, IL-1β, IL-6, and PGE2 productions, and MEMix pretreatment attenuated these productions by LPS concentration dependently. However, pretreatment with MEMix did not change COX-2 expression by LPS in Raw 264.7 cells. MEMix showed in vitro anti-oxidant and anti-inflammatory activities. MEMix would be useful candidate agent against inflammation.

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