Journal of the Korean Applied Science and Technology (한국응용과학기술학회지)

The Korean Society of Applied Science and Technology (한국응용과학기술학회)
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Antioxidant and Cytotoxicity in Skin Cell of the Trichosanthis Cucumeroidis Radix Extract

쥐참외뿌리 추출물의 항산화 및 피부 세포에서의 세포 독성 연구

  • You, Seon-Hee (Department of Beauty Stylist Major in Skin Care, Yeonsung University) ;
  • Moon, Ji-Sun (Department of Medical Beauty Care Jungwon University)
  • 유선희 (연성대학교 뷰티스타일리스트과) ;
  • 문지선 (중원대학교 의료뷰티케어과)
  • Received : 2022.05.31
  • Accepted : 2022.06.27
  • Published : 2022.06.30
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Abstract

We tried to check the antioxidant activity and toxicity of trichosanthis cucumeroidis radix extracts in skin cells, and check the possibility of their use as a functional material that can be effectively used on the skin. Total polyphenol and total flavonoid content, which are indicators of antioxidant activity of trichosanthis cucumeroidis radix extracts, were confirmed, and cytotoxicity was confirmed using Neutral red assay in the skin. As a result of the study, the content of total polyphenols and total flavonoids increased concentration-dependent. High survival rates in fibroblast HDF cells were identified, and cell survival rates were significantly lowered from 5 ㎍/mL in melanocytes B16F10 melanoma cells and inflammation-related macrophages RAW 264.7 cells. He results of this study are believed to be available as basic data for antioxidant activity of trichosanthis cucumeroidis radix extracts and skin cells.

쥐참외뿌리 추출물이 항산화 활성 및 피부 세포에서의 독성을 확인하여, 피부에 효과적으로 사용할 수 있는 기능성 소재로써의 활용 가능성을 확인해 보고자 하였다. 쥐참외뿌리 추출물의 항산화 활성의 지표가 되는 총 폴리페놀과 총 플라보노이드 함량을 확인하였고, 피부에서의 Neutral red assay를 이용하여 세포 독성을 확인하였다. 연구 결과, 총 폴리페놀과 총 플라보노이드의 함량은 농도 의존적으로 증가하였다. 섬유아 세포인 HDF cell에서의 높은 생존율이 확인되었으며, B16F10 melanoma cel와 RAW 264.7 cell에서는 5 ㎍/mL부터 세포 생존율이 유의하게 낮아지는 것이 확인되었다. 본 연구 결과는 쥐참외뿌리 추출물의 항산화 활성 및 피부 세포에서의 기초적인 자료로 사용가능할 것으로 사료되어 진다.

Keywords

References

  1. S. J. Lee, E. J. Song, S. Y. Lee, K. B. Kim, W. R. S. J. Kim, S. Y. Yoon, "Antioxidant activity of leaf, stem and root extracts from Orostachysjaponicus and their heat and pH stabilities". J Korean Soc Food Sci Nutr, Vol.38 pp. 1571-1579, (2009). https://doi.org/10.3746/JKFN.2009.38.11.1571
  2. H. M. Chiang, H. C. Chen, T. J. Lin, I. C. Shih, K. C. Wen, "Michelia alba extract attenuates UVB-induced expression of matrix metalloproteinases via MAP kinase pathway in human dermal fibroblasts", Food and Chemical Toxicology, Vol.50 pp. 4260-4269, (2012). https://doi.org/10.1016/j.fct.2012.08.018
  3. J. O. Kim, C. W. Lee, E. K. Kim, S. J. Lee, N. H. Park, H. S. Kim, H. K. Kim, K. H. Char, Y. P. Jang, J. W. Kim. "Inhibition effect of Gynura procumbens extract on UV-B-induced matrix-metalloproteinase expression in human dermal fibroblasts", Journal of Ethnopharmacology, Vol.137 pp. 427-433, (2011). https://doi.org/10.1016/j.jep.2011.04.072
  4. B. K. Han, "The role of active oxygen in the aging process", Korea Institute of Science and Technology, Vol.6 No.2 pp. 9-14, (1999).
  5. J. Regnstrom, J. Nilsson, P. Tornvall, C. Landou, A. Hamsten, "Susceptibility to low-density lipoprotein oxidation and coronaryatherosclerosis in man". Lancet Vol.339 pp. 1183-1186, (1992). https://doi.org/10.1016/0140-6736(92)91129-V
  6. D. Feskanich, R. G. Ziegler, D. S. Michaud, E. L. Giovannucci, F. E. Speizer, W. C. Willett, "Prospective study of fruit and vegetable consumptionand risk of lung cancer among men and women", J Natl Cancer Inst, Vol.92 pp. 1812-123, (1998).
  7. X. Tianhan, Rustum Roy, "Studying Traditional Chinese Medicine", American Association for the Advancement of Science, Vol.300 pp. 740-741, (2003). https://doi.org/10.1126/science.300.5620.740
  8. O. Folin, W. Denis. "On phosphotungstic-phosphomolybdic compounds as color reagents", The Journal of Biological Chemistry. Vol.12 pp. 239-243, (1912). https://doi.org/10.1016/S0021-9258(18)88697-5
  9. M. I. Moreno, M. I. Isla, A. R. Sampietro, M. A. Vattuone. "Comparison of the free radical-scavenging activity of propolis from several regions of Argentina". Journal of Ethnopharmacology, Vol.71 pp. 109-114, (2000). https://doi.org/10.1016/S0378-8741(99)00189-0
  10. Y. H. Kang, Y. K. Park, G. D. Lee, "The nitrite scavenging and electrondonating ability of phenolic compounds". Korean J Food Sci Technol, Vol.28 pp. 232-239, (1996).
  11. J. Y. Cha, H. J. Kim, C. H. Chung, S. C. Young. Antioxidative Activities and Contents of Polyphenolic Compound of Cudrania tricuspidata. J. Korean. Soc. Food Sci. Nutr, Vol.28 No.6 pp. 1310-1315, (1999).