Mycobiology

The Korean Society of Mycology (한국균학회)
권호 표지
DOI QR코드

DOI QR Code

Effect of Temperature, pH, and Media on the Mycelial Growth of Tuber koreanum

  • Ju-Hui Gwon (Department of Biology Education, Korea National University of Education) ;
  • Hyeok Park (Department of Biology Education, Korea National University of Education) ;
  • Ahn-Heum Eom (Department of Biology Education, Korea National University of Education)
  • Received : 2022.05.24
  • Accepted : 2022.08.09
  • Published : 2022.08.31
Download PDF
⟨ Previous Next ⟩

Abstract

Members of the genus Tuber are ectomycorrhizal fungi; this genus includes more than 180 species worldwide. In the present study, the optimal pH, temperature, and medium suitable for the mycelial growth of the Korean truffle, Tuber koreanum, were determined. Mycelium of T. koreanum, isolated from fruiting bodies collected in Korea, was used to investigate the effects of these environmental factors. The results showed that malt extract agar and potato dextrose agar were the most suitable for the mycelial growth of T. koreanum when cultured at a pH of 6.0 at 25 ℃ for 30 days.

Keywords

Acknowledgement

This work was supported by the Korea Institute of Planning and Evaluation for Technology in Food, Agriculture, Forestry and Fisheries (IPET) funded by the Ministry of Agriculture, Food and Rural Affairs (MAFRA) (IPET319106052HD050).

References

  1. Urban A. Truffles and small mammals. True truffle (Tuber spp.) in the world. Cham: Springer; 2016. p. 353-373.
  2. Bonito GM, Trappe JM, Rawlinson P, et al. Improved resolution of major clades within Tuber and taxonomy of species within the Tuber gibbosum complex. Mycologia. 2010;102(5):1042-1057. https://doi.org/10.3852/09-213
  3. Bonito GM, Gryganskyi AP, Trappe JM, et al. A global meta-analysis of Tuber ITS rDNA sequences: species diversity, host associations and long-distance dispersal. Mol Ecol. 2010;19(22):4994-5008. https://doi.org/10.1111/j.1365-294X.2010.04855.x
  4. Tsiaras S, Dragoslis A, Papathanasiou J, editors. Fuzzy multiple criteria analysis for selecting the optimum tree species for truffle cultivation in Greece. In: International Workshop "Information Technology, Sustainable Development, Scientific Network and Nature Protection". 18th Panhellenic Forestry Congress; 2017; Edessa, Greece.
  5. Thomas PW. The role of pH in Tuber aestivum syn. uncinatum mycorrhiza development within commercial orchards. Acta Mycol. 2013;47(2):161-167. https://doi.org/10.5586/am.2012.019
  6. Bonet JA, Oliach D, Fischer C, et al. Cultivation methods of the black truffle, the most profitable Mediterranean non-wood forest product; a state of the art review. Modelling, valuing and managing Mediterranean Forest ecosystems for non-timber goods and services. EFI Proc. 2009;57:57-71.
  7. Ceccaroli P, Saltarelli R, Cesari P, et al. Effects of different carbohydrate sources on the growth of Tuber borchii Vittad. mycelium strains in pure culture. Mol Cell Biochem. 2001;218(1/2):65-70. https://doi.org/10.1023/A:1007265423786
  8. Mamoun M, Olivier JM. Influence du substrat carbone et de la forme d'azote mineral sur la croissance de Tuber melanosporum (Vitt) en culture pure. Application a la production de biomasse mycelienne. Agronomie. 1991;11(6):521-527. https://doi.org/10.1051/agro:19910609
  9. Park H, Gwon JH, Lee JC, et al. Report on a new truffle species, Tuber koreanum sp. nov., from Korea. Mycobiology. 2021;49(6):527-533. https://doi.org/10.1080/12298093.2021.1992089
  10. Nadim M, Saidi N, Hasani I, et al. Effects of some environmental parameters on mycelia growth of Finnish truffle Tuber maculatum. Int J Adv Eng Sci Appl Math. 2016;3:2394-3661.
  11. Leonardi P, Iotti M, Zeppa SD, et al. Morphological and functional changes in mycelium and mycorrhizas of Tuber borchii due to heat stress. Fungal Ecol. 2017;29:20-29. https://doi.org/10.1016/j.funeco.2017.05.003
  12. Rosa-Gruszecka A, Hilszczanska D, Pacioni G. Virtual truffle hunting-a new method of burgundy truffle (Tuber aestivum Vittad.) site typing. Forests. 2021;12(9):1239.
  13. Mello A, Murat C, Bonfante P. Truffles: much more than a prized and local fungal delicacy. FEMS Microbiol Lett. 2006;260:1-8. https://doi.org/10.1111/j.1574-6968.2006.00252.x
  14. Nakano S, Kinoshita A, Obase K, et al. Influence of pH on in vitro mycelial growth in three Japanese truffle species: Tuber japonicum, T. himalayense, and T. longispinosum. Mycoscience. 2020;61(2):58-61. https://doi.org/10.1016/j.myc.2019.12.001
  15. Mischiati P, Fontana A. In vitro culture of Tuber magnatum mycelium isolated from mycorrhizas. Mycol Res. 1993;97(1):40-44. https://doi.org/10.1016/S0953-7562(09)81110-6
  16. Bustan A, Ventura Y, Kagan-Zur V, et al. Optimized conditions for mycorrhiza formation between the pink rockrose (Cistus incanus) and the black Perigord truffle (Tuber melanosporum). Isr J Plant Sci. 2006;54(2):87-96. https://doi.org/10.1560/IJPS_54_2_87
  17. Iotti M, Leonardi P, Vitali G, et al. Effect of summer soil moisture and temperature on the vertical distribution of Tuber magnatum mycelium in soil. Biol Fertil Soils. 2018;54(6):707-716. https://doi.org/10.1007/s00374-018-1296-3
  18. Cruz-Suarez LE, Ricque-Marie D, Pinal-Mansilla JD, et al. Effect of different carbohydrate sources on the growth of Penaeus vannamei: economical impact. Aquaculture. 1994;123(3-4):349-360. https://doi.org/10.1016/0044-8486(94)90070-1
  19. Saltarelli R, Ceccaroli P, Polidori E, et al. A high concentration of glucose inhibits Tuber borchii mycelium growth: a biochemical investigation. Mycol Res. 2003;107:72-76. https://doi.org/10.1017/S0953756202007062