Journal of Microbiology and Biotechnology

The Korean Society for Microbiology and Biotechnology (한국미생물·생명공학회)
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Deinococcus radiodurans R1 Lysate Induces Tolerogenic Maturation in Lipopolysaccharide-Stimulated Dendritic Cells and Protects Dextran Sulfate Sodium-Induced Colitis in Mice

  • Song, Ha-Yeon (Advanced Radiation Technology Institute, Korea Atomic Energy Research Institute) ;
  • Han, Jeong Moo (Advanced Radiation Technology Institute, Korea Atomic Energy Research Institute) ;
  • Kim, Woo Sik (Functional Biomaterial Research Center, Korea Research Institute of Bioscience and Biotechnology) ;
  • Lee, Ji Hee (Division of Pathogen Resource Management, Center for Vaccine Development Support, National Institute of Infectious Disease, National Institute of Health (NIH), Korea Disease Control and Prevention Agency) ;
  • Park, Woo Yong (Department of Pharmacology, College of Korean Medicine, Kyung Hee University) ;
  • Byun, Eui-Baek (Advanced Radiation Technology Institute, Korea Atomic Energy Research Institute) ;
  • Byun, Eui-Hong (Department of Food Science and Technology, Kongju National University)
  • Received : 2022.03.04
  • Accepted : 2022.06.02
  • Published : 2022.07.28
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Abstract

Deinococcus radiodurans is an extremophilic bacterium that can thrive in harsh environments. This property can be attributed to its unique metabolites that possess strong antioxidants and other pharmacological properties. To determine the potential of D. radiodurans R1 lysate (DeinoLys) as a pharmacological candidate for inflammatory bowel disease (IBD), we investigated the antiinflammatory activity of DeinoLys in bone marrow-derived dendritic cells (BMDCs) and a colitis mice model. Lipopolysaccharide (LPS)-stimulated BMDCs treated with DeinoLys exhibited alterations in their phenotypic and functional properties by changing into tolerogenic DCs, including strongly inhibited proinflammatory cytokines (TNF-α and IL-12p70) and surface molecule expression and activated DC-induced T cell proliferation/activation with high IL-10 production. These phenotypic and functional changes in BMDCs induced by DeinoLys in the presence of LPS were abrogated by IL-10 neutralization. Furthermore, oral administration of DeinoLys significantly reduced clinical symptoms against dextran sulfate sodium-induced colitis, including body weight loss, disease activity index, histological severity in colon tissue, and lower myeloperoxidase level in mice. Our results establish DeinoLys as a potential anti-inflammatory candidate for IBD therapy.

Keywords

Acknowledgement

This work was supported by the National Research Foundation of Korea grant funded by Korea government (NRF-NRF2022R1A2C4001251), the Internal R&D program of KAERI (523210) funded by Ministry of Science and ICT (MSIT), and research grant from Kongju National University in 2021 under grant 2021-0419-01.

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