Journal of Microbiology and Biotechnology

The Korean Society for Microbiology and Biotechnology (한국미생물·생명공학회)
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Effect of Methyl Gallate on 1-Nitropyrene-Induced Keratinocyte Toxicity in a Human and Canine Skin Model

  • Lee, Woo Jin (School of Food Science and Biotechnology, Kyungpook National University) ;
  • Kim, Min Jeong (School of Food Science and Biotechnology, Kyungpook National University) ;
  • Choi, Hyun-Wook (Department of Functional Food and Biotechnology, Jeonju University) ;
  • Lee, Jeong Jae (Institute of Agricultural Science and Technology, Kyungpook National University) ;
  • Jung, Sung Keun (School of Food Science and Biotechnology, Kyungpook National University)
  • Received : 2022.06.07
  • Accepted : 2022.06.27
  • Published : 2022.07.28
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Abstract

The skin, which is the largest organ of the human body, is in direct contact with pollutants in the surrounding atmosphere. Meanwhile, 1-nitropyrene (1-NP), the most abundant nitro-polycyclic aromatic hydrocarbon found in particulate matter, is known to have carcinogenic effects; however, studies on its toxicity in human and canine skin are still needed. In this study, we investigated 1-NP-induced apoptosis and inflammatory pathways in HaCaT cells. In addition, we also measured the cytoprotective effect of methyl gallate (MG), which is widely distributed in medicinal and edible plants and is well known for its anti-inflammatory and antioxidant properties. MG inhibited 1-NP-induced cell death and apoptosis pathways, including the cleavage of PARP and activation of caspase-3, -7, and -9. MG also suppressed 1-NP-induced COX-2 expression and phosphorylation of mitogen-activated protein kinases (MAPKs) and MAPK kinases (MAPKKs). Our findings suggest that 1-NP induces skin toxicity in human and canine through apoptosis and inflammatory responses, and moreover, that this can be prevented by treatment with MG.

Keywords

Acknowledgement

This project was supported by the Ministry of Oceans and Fisheries of Korea (No.20210386), and by the National Research Foundation of Korea grant funded by the Korean government (NRF-2021M3E5E6022715, NRF-2021R1F1A1062889).

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