The Journal of Korean Medicine Ophthalmology and Otolaryngology and Dermatology (한방안이비인후피부과학회지)

The Society of Korean Medicine Ophthalmology, Otolaryngology & Dermatology (대한한방안이비인후피부과학회)
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Research of the Anti-inflammatory Effects of Hwadokdan on Raw 264.7 Cells

Raw 264.7 세포에서 화독단(化毒丹)의 항염증 효능에 대한 연구

  • Shin, Ki Seok (Dep. of Korean Medicine Ophthalmology & Otolaryngology & Dermatology, Daegu Hanny University) ;
  • Hwangbo, Min (Dep. of Korean Medicine Ophthalmology & Otolaryngology & Dermatology, Daegu Hanny University) ;
  • Jee, Seon Young (Dep. of Korean Medicine Ophthalmology & Otolaryngology & Dermatology, Daegu Hanny University)
  • 신기석 (대구한의대학교 한의과대학 안이비인후피부과학교실) ;
  • 황보민 (대구한의대학교 한의과대학 안이비인후피부과학교실) ;
  • 지선영 (대구한의대학교 한의과대학 안이비인후피부과학교실)
  • Received : 2022.06.30
  • Accepted : 2022.07.29
  • Published : 2022.08.25
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Abstract

Objectives : The purpose of this study was to evaluate the anti-Inflammatory effect of Hwadokdan(HDD) extracted with water. Methods : We pretreated LPS-activated Raw 264.7 cell with HDD and anti-inflammatory effect of HDD is investigated by measuring NO production, relative iNOS, and TNF-α, IL-6, IL-1β, PGE2 productions level, p-IκBα, IκBα, NFκB. Results : HDD reduced NO production and iNOS protein, TNF-α, IL-6 level at concentration of 100㎍/㎖ and 300㎍/㎖ in LPS-activated Raw 264.7 cell significantly. There wasn't a significant cytotoxicity in MTT assay. And HDD reduced IL-1β level at concentration of 300㎍/㎖ in LPS-activated Raw 264.7 cell significantly. HDD reduced p-IκBα and NFκB protein level at concentration of 300㎍/㎖ in LPS-activated Raw 264.7 cell significantly. And HDD enhanced IκBα protein level at concentration of 100㎍/㎖ and 300㎍/㎖ in LPS-activated Raw 264.7 cell significantly. Conclusions : These suggests that HDD can be used as a therapeutic drug for various inflammatory diseases.

Keywords

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