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The Korean Society of Phycology (한국조류학회(藻類))
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Statistical optimization of phytol and polyunsaturated fatty acid production in the Antarctic microalga Micractinium variabile KSF0031

  • Kim, Eun Jae (Division of Life Sciences, Korea Polar Research Institute) ;
  • Chae, Hyunsik (Division of Life Sciences, Korea Polar Research Institute) ;
  • Koo, Man Hyung (Research Unit of Cryogenic Novel Material, Korea Polar Research Institute) ;
  • Yu, Jihyeon (Division of Life Sciences, Korea Polar Research Institute) ;
  • Kim, Hyunjoong (Division of Life Sciences, Korea Polar Research Institute) ;
  • Cho, Sung Mi (Division of Life Sciences, Korea Polar Research Institute) ;
  • Hong, Kwang Won (R&D Department, Microalgae Ask Us Co., Ltd.) ;
  • Lee, Joo Young (Department of Polar Sciences, University of Science and Technology) ;
  • Youn, Ui Joung (Division of Life Sciences, Korea Polar Research Institute) ;
  • Kim, Sanghee (Division of Life Sciences, Korea Polar Research Institute) ;
  • Choi, Han-Gu (Division of Life Sciences, Korea Polar Research Institute) ;
  • Han, Se Jong (Division of Life Sciences, Korea Polar Research Institute)
  • Received : 2021.10.20
  • Accepted : 2022.04.01
  • Published : 2022.06.15
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Abstract

Polar microorganisms produce physiologically active substances to adapt to harsh environments, and these substances can be used as biomedical compounds. The green microalga Micractinium variabile KSF0031, which was isolated from Antarctica, produced phytol, a natural antimicrobial agent. Furthermore, several polyunsaturated fatty acids (PUFAs), including omega-3, exhibit antioxidant properties. Here statistical methods (Plackett-Burman design and Box-Behnken design) were used to optimize the culture medium of KSF0031 to improve biomass production, and K2HPO4, MgSO4·7H 2O, and ammonium ferric citrate green (AFCg) were selected as significant components of the culture medium. Changes in the concentration of K2HPO4 and MgSO4·7H 2O as positive factors and AFCg as a negative factor affected cell growth to a remarkable degree. The biomass production in a 100 L culture using the optimized medium for 24 d at 18℃ was improved by 37.5% compared to that obtained using the original BG-11 medium. The quantities of PUFAs and phytol obtained were 13 mg g-1 dry cell weight (DCW) and 10.98 mg g-1 DCW, which represent improved yields of 11.70% and 48.78%, respectively. The results of this study could contribute to an improved production of phytol and fatty acids from Antarctic microalgae in the biomedical industry.

Keywords

Acknowledgement

The research was supported by Grants from the Korea Polar Research Institute (PE22140 and PE19910).

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