Restorative Dentistry and Endodontics

The Korean Academy of Conservative Dentistry (대한치과보존학회)
권호 표지
DOI QR코드

DOI QR Code

Bonding effects of cleaning protocols and time-point of acid etching on dentin impregnated with endodontic sealer

  • Tatiane Miranda Manzoli (Department of Restorative Dentistry, School of Dentistry, Araraquara, Sao Paulo State University – UNESP) ;
  • Joissi Ferrari Zaniboni (Department of Restorative Dentistry, School of Dentistry, Araraquara, Sao Paulo State University – UNESP) ;
  • Joao Felipe Besegato (Department of Restorative Dentistry, School of Dentistry, Araraquara, Sao Paulo State University – UNESP) ;
  • Flavia Angelica Guiotti (Department of Restorative Dentistry, School of Dentistry, Araraquara, Sao Paulo State University – UNESP) ;
  • Andrea Abi Rached Dantas (Department of Restorative Dentistry, School of Dentistry, Araraquara, Sao Paulo State University – UNESP) ;
  • Milton Carlos Kuga (Department of Restorative Dentistry, School of Dentistry, Araraquara, Sao Paulo State University – UNESP)
  • Received : 2021.03.22
  • Accepted : 2021.10.21
  • Published : 2022.05.31
Download PDF
⟨ Previous Next ⟩

Abstract

Objectives: This study aimed to investigate the bonding effects of cleaning protocols on dentin impregnated with endodontic sealer residues using ethanol (E) or xylol (X). The effects of dentin acid etching immediately (I) or 7 days (P) after cleaning were also evaluated. For bonding to dentin, universal adhesive (Scotchbond Universal; 3M ESPE) was used. The persistence of sealer residues, hybrid layer formation and microshear bond strength were the performed analysis. Materials and Methods: One hundred and twenty bovine dentin specimens were allocated into 4 groups (n = 10): G1 (E+I); G2 (X+I); G3 (E+P); and G4 (X+P). The persistence of sealer residues was evaluated by SEM. Confocal laser scanning microscopy images were taken to measure the formed hybrid layer using the Image J program. For microshear bond strength, 4 resin composite cylinders were placed over the dentin after the cleaning protocols. ANOVA followed by Tukey test and Kruskal-Wallis followed by Dunn test were used for parametric and non-parametric data, respectively (α = 5%). Results: G2 and G4 groups showed a lower persistence of residues (p < 0.05) and thicker hybrid layer than the other groups (p < 0.05). No bond strength differences among all groups were observed (p > 0.05). Conclusions: Dentin cleaning using xylol, regardless of the time-point of acid etching, provided lower persistence of residues over the surface and thicker hybrid layer. However, the bond strength of the universal adhesive system in etch-and-rinse strategy was not influenced by the cleaning protocols or time-point of acid etching.

Keywords

Acknowledgement

This study was financed in part by the Coordenacao de Aperfeicoamento de Pessoal de Nivel Superior (CAPES) - Finance code 001.

References

  1. Roberts S, Kim JR, Gu LS, Kim YK, Mitchell QM, Pashley DH, Tay FR. The efficacy of different sealer removal protocols on bonding of self-etching adhesives to AH plus-contaminated dentin. J Endod 2009;35:563-567.
  2. Bandeca MC, Kuga MC, Diniz AC, Jordao-Basso KC, Tonetto MR. Effects of the residues from the endodontic sealers on the longevity of esthetic restorations. J Contemp Dent Pract 2016;17:615-617. https://doi.org/10.5005/jp-journals-10024-1899
  3. Bakaus TE, Gruber YL, Reis A, Gomes JC, Gomes GM. Bonding properties of universal adhesives to root canals prepared with different rotary instruments. J Prosthet Dent 2019;121:298-305. https://doi.org/10.1016/j.prosdent.2018.02.013
  4. Kuga MC, Faria G, Rossi MA, do Carmo Monteiro JC, Bonetti-Filho I, Berbert FL, Keine KC, So MV. Persistence of epoxy-based sealer residues in dentin treated with different chemical removal protocols. Scanning 2013;35:17-21. https://doi.org/10.1002/sca.21030
  5. Mushtaq M, Masoodi A, Farooq R, Yaqoob Khan F. The dissolving ability of different organic solvents on three different root canal sealers: in vitro study. Iran Endod J 2012;7:198-202.
  6. Korasli D, Ziraman F, Ozyurt P, Cehreli SB. Microleakage of self-etch primer/adhesives in endodontically treated teeth. J Am Dent Assoc 2007;138:634-640. https://doi.org/10.14219/jada.archive.2007.0235
  7. Demirbuga S, Pala K, Topcuoglu HS, Cayabatmaz M, Topcuoglu G, Ucar EN. Effect of different gutta-percha solvents on the microtensile bond strength of various adhesive systems to pulp chamber dentin. Clin Oral Investig 2017;21:627-633. https://doi.org/10.1007/s00784-016-1929-6
  8. Kaczor K, Krasowski M, Lipa S, Sokolowski J, Nowicka A. How do the etching mode and thermomechanical loading influence the marginal integrity of universal adhesives? Oper Dent 2020;45:306-317.
  9. Leite MLAES, Costa CAS, Duarte RM, Andrade AKM, Soares DG. Bond strength and cytotoxicity of a universal adhesive according to the hybridization strategies to dentin. Braz Dent J 2018;29:68-75. https://doi.org/10.1590/0103-6440201801698
  10. Munoz MA, Luque I, Hass V, Reis A, Loguercio AD, Bombarda NH. Immediate bonding properties of universal adhesives to dentine. J Dent 2013;41:404-411. https://doi.org/10.1016/j.jdent.2013.03.001
  11. Oliveira SS, Pugach MK, Hilton JF, Watanabe LG, Marshall SJ, Marshall GW Jr. The influence of the dentin smear layer on adhesion: a self-etching primer vs. a total-etch system. Dent Mater 2003;19:758-767. https://doi.org/10.1016/S0109-5641(03)00023-X
  12. Jordao-Basso KC, Kuga MC, Bandeca MC, Duarte MA, Guiotti FA. Effect of the time-point of acid etching on the persistence of sealer residues after using different dental cleaning protocols. Braz Oral Res 2016;30:e133.
  13. Langer A, Ilie N. Dentin infiltration ability of different classes of adhesive systems. Clin Oral Investig 2013;17:205-216. https://doi.org/10.1007/s00784-012-0694-4
  14. Hass V, Cardenas A, Siqueira F, Pacheco RR, Zago P, Silva DO, Bandeca MC, Loguercio AD. Bonding performance of universal adhesive systems applied in etch-and-rinse and self-etch strategies on natural dentin caries. Oper Dent 2019;44:510-520. https://doi.org/10.2341/17-252-L
  15. Wagner A, Wendler M, Petschelt A, Belli R, Lohbauer U. Bonding performance of universal adhesives in different etching modes. J Dent 2014;42:800-807. https://doi.org/10.1016/j.jdent.2014.04.012
  16. Rosa WL, Piva E, Silva AF. Bond strength of universal adhesives: a systematic review and meta-analysis. J Dent 2015;43:765-776. https://doi.org/10.1016/j.jdent.2015.04.003
  17. Morais JMP, Victorino KR, Escalante-Otarola WG, Jordao-Basso KCF, Palma-Dibb RG, Kuga MC. Effect of the calcium silicate-based sealer removal protocols and time-point of acid etching on the dentin adhesive interface. Microsc Res Tech 2018;81:914-920. https://doi.org/10.1002/jemt.23056
  18. Kuga MC, So MV, De Faria-junior NB, Keine KC, Faria G, Fabricio S, Matsumoto MA. Persistence of resinous cement residues in dentin treated with different chemical removal protocols. Microsc Res Tech 2012;75:982-985. https://doi.org/10.1002/jemt.22023
  19. Martos J, Bassotto AP, Gonzalez-Rodriguez MP, Ferrer-Luque CM. Dissolving efficacy of eucalyptus and orange oil, xylol and chloroform solvents on different root canal sealers. Int Endod J 2011;44:1024-1028. https://doi.org/10.1111/j.1365-2591.2011.01912.x
  20. Shenoi PR, Badole GP, Khode RT. Evaluation of softening ability of Xylene & Endosolv-R on three different epoxy resin based sealers within 1 to 2 minutes - an in vitro study. Restor Dent Endod 2014;39:17-23. https://doi.org/10.5395/rde.2014.39.1.17
  21. Kuga MC, So MV, De Campos EA, Faria G, Keine KC, Dantas AA, Faria NB Jr. Persistence of endodontic methacrylate-based cement residues on dentin adhesive surface treated with different chemical removal protocols. Microsc Res Tech 2012;75:1432-1436. https://doi.org/10.1002/jemt.22086
  22. Guzman-Armstrong S, Armstrong SR, Qian F. Relationship between nanoleakage and microtensile bond strength at the resin-dentin interface. Oper Dent 2003;28:60-66. 
  23. Cardoso MV, de Almeida Neves A, Mine A, Coutinho E, Van Landuyt K, De Munck J, Van Meerbeek B. Current aspects on bonding effectiveness and stability in adhesive dentistry. Aust Dent J 2011;56 Supplement 1:31-44. https://doi.org/10.1111/j.1834-7819.2011.01294.x
  24. Pane ES, Palamara JE, Messer HH. Critical evaluation of the push-out test for root canal filling materials. J Endod 2013;39:669-673. https://doi.org/10.1016/j.joen.2012.12.032
  25. Yoshiyama M, Urayama A, Kimochi T, Matsuo T, Pashley DH. Comparison of conventional vs self-etching adhesive bonds to caries-affected dentin. Oper Dent 2000;25:163-169.
  26. Hanabusa M, Mine A, Kuboki T, Momoi Y, Van Ende A, Van Meerbeek B, De Munck J. Bonding effectiveness of a new 'multi-mode' adhesive to enamel and dentine. J Dent 2012;40:475-484. https://doi.org/10.1016/j.jdent.2012.02.012
  27. Perdigao J, Sezinando A, Monteiro PC. Laboratory bonding ability of a multi-purpose dentin adhesive. Am J Dent 2012;25:153-158.
  28. Perdigao J, Kose C, Mena-Serrano AP, De Paula EA, Tay LY, Reis A, Loguercio AD. A new universal simplified adhesive: 18-month clinical evaluation. Oper Dent 2014;39:113-127. https://doi.org/10.2341/13-045-C
  29. Chen C, Niu LN, Xie H, Zhang ZY, Zhou LQ, Jiao K, Chen JH, Pashley DH, Tay FR. Bonding of universal adhesives to dentine--old wine in new bottles? J Dent 2015;43:525-536. https://doi.org/10.1016/j.jdent.2015.03.004
  30. Heintze SD, Rousson V, Mahn E. Bond strength tests of dental adhesive systems and their correlation with clinical results - a meta-analysis. Dent Mater 2015;31:423-434. https://doi.org/10.1016/j.dental.2015.01.011
  31. Hirokane E, Takamizawa T, Kasahara Y, Ishii R, Tsujimoto A, Barkmeier WW, Latta MA, Miyazaki M. Effect of double-layer application on the early enamel bond strength of universal adhesives. Clin Oral Investig 2021;25:907-921.
  32. Fujiwara S, Takamizawa T, Barkmeier WW, Tsujimoto A, Imai A, Watanabe H, Erickson RL, Latta MA, Nakatsuka T, Miyazaki M. Effect of double-layer application on bond quality of adhesive systems. J Mech Behav Biomed Mater 2018;77:501-509. https://doi.org/10.1016/j.jmbbm.2017.10.008
  33. Taschner M, Kummerling M, Lohbauer U, Breschi L, Petschelt A, Frankenberger R. Effect of double-layer application on dentin bond durability of one-step self-etch adhesives. Oper Dent 2014;39:416-426.  https://doi.org/10.2341/13-168-L