Restorative Dentistry and Endodontics

The Korean Academy of Conservative Dentistry (대한치과보존학회)
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Cytotoxicity of two self-adhesive resin cements and their interference in the phagocytic activity of murine macrophages

  • Danilo Couto da Silva (Department of Restorative Dentistry, Faculty of Dentistry, Universidade Federal de Minas Gerais (UFMG)) ;
  • Leonardo Gomes Vaz (Department of Biochemistry and Immunology, Institute of Biological Sciences, Universidade Federal de Minas Gerais (UFMG)) ;
  • Warley Luciano Fonseca Tavares (Department of Restorative Dentistry, Faculty of Dentistry, Universidade Federal de Minas Gerais (UFMG)) ;
  • Leda Quercia Vieira (Department of Biochemistry and Immunology, Institute of Biological Sciences, Universidade Federal de Minas Gerais (UFMG)) ;
  • Ricardo Reis de Oliveira (Department of Restorative Dentistry, Faculty of Dentistry, Universidade Federal de Minas Gerais (UFMG)) ;
  • Antonio Paulino Ribeiro Sobrinho (Department of Restorative Dentistry, Faculty of Dentistry, Universidade Federal de Minas Gerais (UFMG))
  • Received : 2022.01.04
  • Accepted : 2022.02.15
  • Published : 2022.08.31
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Abstract

Objectives: This study aimed to evaluate in vitro the effects of the self-adhesive resin cements RelyX U200 (3M ESPE) and seT PP (SDI Limited) on murine macrophages and the interference of the photoactivation. Materials and Methods: Cell viability assays, cell adherence, yeast phagocytosis of Saccharomyces boulardii and production of reactive oxygen species (ROS) were performed in the presence of capillaries containing the respective self-adhesive cement when photoactivated or not. Results: After long periods of contact, both types of cements, when not photoactivated, are more cytotoxic for macrophages. The seT PP cement when only chemically activated seems to interfere more negatively in the process of phagocytosis of yeasts S. boulardii. Both types of cements interfere in the cell adhesion process, independent of photoactivation. None of the types of cements tested was able to induce the production of ROS. Conclusions: Our results highlight the great importance of the photoactivation of self-adhesive resin cements in the dental clinic, since RelyX U200, when photoactivated, presented the best results within the evaluated parameters.

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References

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