Journal of Korean Dental Science

Korean Academy of Dental Science (대한치의학회)
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Decellularized Non-cross-linked Collagen Membranes for Guided Bone Regeneration in Rabbit Calvarial Defects

  • Jeon, Su-Hee (Department of Periodontology, Research Institute for Periodontal Regeneration, Yonsei University College of Dentistry) ;
  • Lee, Da-Na (Department of Periodontology, Research Institute for Periodontal Regeneration, Yonsei University College of Dentistry) ;
  • Seo, Young-Wook (Department of Periodontology, Research Institute for Periodontal Regeneration, Yonsei University College of Dentistry) ;
  • Park, Jin-Young (Department of Periodontology, Research Institute for Periodontal Regeneration, Yonsei University College of Dentistry) ;
  • Paik, Jeong-Won (Department of Periodontology, Research Institute for Periodontal Regeneration, Yonsei University College of Dentistry) ;
  • Cha, Jae-Kook (Department of Periodontology, Research Institute for Periodontal Regeneration, Yonsei University College of Dentistry) ;
  • Choi, Seong-Ho (Department of Periodontology, Research Institute for Periodontal Regeneration, Yonsei University College of Dentistry)
  • Received : 2022.04.05
  • Accepted : 2022.06.07
  • Published : 2022.06.30
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Abstract

Purpose: The aim of this study was to evaluate the bio-durability and bone regeneration capacity of the non-cross-linked collagen membrane in rabbit calvarial defect models. Materials and Methods: Four circular defects with 8 mm diameter were made in each of calvarium of 10 male rabbits. The following groups was randomly assigned to each defect - 1) Control, 2) membrane group containing non-cross-linked collagen membrane only (M), 3) bone graft group (B), 4) bone graft with membrane group (B+M). Animals were sacrificed and samples were harvested at 2 weeks (n=5) and 8 weeks (n=5). Histologic sections were prepared and histomorphometric analysis was performed. Result: Histologic results showed well adaptation of the non-cross-linked membrane on each defect and normal healing response at 2 weeks. At 8 weeks, the membranes were partially biodegraded. Histomorphometrically, B and B+M group showed the significantly greater total augmented area (B+M group, 10.44±1.49, P=0.016; B group, 9.13±0.53, P=0.032) and new bone formation (B+M group, 2.89±0.93, P=0.008; B group, 2.85±1.15, P=0.008) compared to control group. Collapsing of the central portion of the membrane, membrane group showed greater value in new bone formation at 8 weeks (1.78±0.68, P=0.032). Conclusion: Within the limitations of this study, the non-cross-linked collagen membrane fabricated using the improved decellularized method was shown to be effective for the regeneration of calvarial bone defects. In addition, prolonged barrier function might be provided using this collagen membrane.

Keywords

Acknowledgement

This work was supported by the National Research Foundation of Korea (NRF) grant funded by the Korea government (Ministry of Science, ICT & Future Planning) (No. NRF2017R1A2B4002782).

References

  1. Retzepi M, Donos N. Guided Bone Regeneration: biological principle and therapeutic applications. Clin Oral Implants Res. 2010; 21: 567-76. https://doi.org/10.1111/j.1600-0501.2010.01922.x
  2. Sanz-Sanchez I, Ortiz-Vigon A, Sanz-Martin I, Figuero E, Sanz M. Effectiveness of lateral bone augmentation on the alveolar crest dimension: a systematic review and meta-analysis. J Dent Res. 2015; 94(9 Suppl): 128S-42S. https://doi.org/10.1177/0022034515594780
  3. Benic GI, Hammerle CH. Horizontal bone augmentation by means of guided bone regeneration. Periodontol 2000. 2014; 66: 13-40. https://doi.org/10.1111/prd.12039
  4. Bunyaratavej P, Wang HL. Collagen membranes: a review. J Periodontol. 2001; 72: 215-29. https://doi.org/10.1902/jop.2001.72.2.215
  5. Chia-Lai PJ, Orlowska A, Al-Maawi S, Dias A, Zhang Y, Wang X, Zender N, Sader R, Kirkpatrick CJ, Ghanaati S. Sugar-based collagen membrane cross-linking increases barrier capacity of membranes. Clin Oral Investig. 2018; 22: 1851-63. https://doi.org/10.1007/s00784-017-2281-1
  6. Jimenez Garcia J, Berghezan S, Carames JMM, Dard MM, Marques DNS. Effect of cross-linked vs noncross-linked collagen membranes on bone: a systematic review. J Periodontal Res. 2017; 52: 955-64. https://doi.org/10.1111/jre.12470
  7. Rothamel D, Schwarz F, Sculean A, Herten M, Scherbaum W, Becker J. Biocompatibility of various collagen membranes in cultures of human PDL fibroblasts and human osteoblast-like cells. Clin Oral Implants Res. 2004; 15: 443-9. https://doi.org/10.1111/j.1600-0501.2004.01039.x
  8. Rothamel D, Schwarz F, Sager M, Herten M, Sculean A, Becker J. Biodegradation of differently crosslinked collagen membranes: an experimental study in the rat. Clin Oral Implants Res. 2005; 16: 369-78. https://doi.org/10.1111/j.1600-0501.2005.01108.x
  9. Bresaola MD, Matsumoto MA, Zahoui A, Biguetti CC, Nary-Filho H. Influence of rapid- and slow-rate resorption collagen membrane in maxillary sinus augmentation. Clin Oral Implants Res. 2017; 28: 320-6.
  10. Barbeck M, Lorenz J, Holthaus MG, Raetscho N, Kubesch A, Booms P, Sader R, Kirkpatrick CJ, Ghanaati S. Porcine dermis and pericardium-based, non-cross-linked materials induce multinucleated giant cells after their in vivo implantation: a physiological reaction? J Oral Implantol. 2015; 41: e267-81. https://doi.org/10.1563/aaid-joi-D-14-00155
  11. Raz P, Brosh T, Ronen G, Tal H. Tensile properties of three selected collagen membranes. Biomed Res Int. 2019; 2019: 5163603.
  12. Ahn JJ, Kim HJ, Bae EB, Cho WT, Choi Y, Hwang SH, Jeong CM, Huh JB. Evaluation of 1-ethyl-3-(3- dimethylaminopropyl) carbodiimide cross-linked collagen membranes for guided bone regeneration in beagle dogs. Materials (Basel). 2020; 13: 4599. https://doi.org/10.3390/ma13204599
  13. Park CS, Kim YJ, Sung SC, Park JE, Choi SY, Kim WH, Kim KH. Study on an effective decellularization technique for cardiac valve, arterial wall and pericardium xenographs: optimization of decellularization. Korean J Thorac Cardiovasc Surg. 2008; 41: 550-62.
  14. Kitayama S, Wong LO, Ma L, Hao J, Kasugai S, Lang NP, Mattheos N. Regeneration of rabbit calvarial defects using biphasic calcium phosphate and a strontium hydroxyapatite-containing collagen membrane. Clin Oral Implants Res. 2016; 27: e206-14. https://doi.org/10.1111/clr.12605
  15. Park JY, Jung IH, Kim YK, Lim HC, Lee JS, Jung UW, Choi SH. Guided bone regeneration using 1-ethyl-3-(3-dimethylaminopropyl) carbodiimide (EDC)-cross-linked type-I collagen membrane with biphasic calcium phosphate at rabbit calvarial defects. Biomater Res. 2015; 19: 15. https://doi.org/10.1186/s40824-014-0024-9
  16. Jensen SS, Broggini N, Hjorting-Hansen E, Schenk R, Buser D. Bone healing and graft resorption of autograft, anorganic bovine bone and beta-tricalcium phosphate. A histologic and histomorphometric study in the mandibles of minipigs. Clin Oral Implants Res. 2006; 17: 237-43. https://doi.org/10.1111/j.1600-0501.2005.01257.x
  17. Jensen SS, Yeo A, Dard M, Hunziker E, Schenk R, Buser D. Evaluation of a novel biphasic calcium phosphate in standardized bone defects: a histologic and histomorphometric study in the mandibles of minipigs. Clin Oral Implants Res. 2007; 18: 752-60. https://doi.org/10.1111/j.1600-0501.2007.01417.x
  18. Hurzeler MB, Kohal RJ, Naghshbandi J, Mota LF, Conradt J, Hutmacher D, Caffesse RG. Evaluation of a new bioresorbable barrier to facilitate guided bone regeneration around exposed implant threads. An experimental study in the monkey. Int J Oral Maxillofac Surg. 1998; 27: 315-20. https://doi.org/10.1016/S0901-5027(05)80623-X
  19. Schwarz F, Rothamel D, Herten M, Sager M, Becker J. Angiogenesis pattern of native and cross-linked collagen membranes: an immunohistochemical study in the rat. Clin Oral Implants Res. 2006; 17: 403-9. https://doi.org/10.1111/j.1600-0501.2005.01225.x
  20. Badylak SF, Taylor D, Uygun K. Whole-organ tissue engineering: decellularization and recellularization of three-dimensional matrix scaffolds. Annu Rev Biomed Eng. 2011; 13: 27-53. https://doi.org/10.1146/annurev-bioeng-071910-124743
  21. Chappard D, Fressonnet C, Genty C, Basle MF, Rebel A. Fat in bone xenografts: importance of the purification procedures on cleanliness, wettability and biocompatibility. Biomaterials. 1993; 14: 507-12. https://doi.org/10.1016/0142-9612(93)90238-W
  22. Owens KW, Yukna RA. Collagen membrane resorption in dogs: a comparative study. Implant Dent. 2001; 10: 49-58. https://doi.org/10.1097/00008505-200101000-00016
  23. Rothamel D, Schwarz F, Fienitz T, Smeets R, Dreiseidler T, Ritter L, Happe A, Zoller J. Biocompatibility and biodegradation of a native porcine pericardium membrane: results of in vitro and in vivo examinations. Int J Oral Maxillofac Implants. 2012; 27: 146-54.
  24. Bozkurt A, Apel C, Sellhaus B, van Neerven S, Wessing B, Hilgers RD, Pallua N. Differences in degradation behavior of two non-cross-linked collagen barrier membranes: an in vitro and in vivo study. Clin Oral Implants Res. 2014; 25: 1403-11. https://doi.org/10.1111/clr.12284
  25. von Arx T, Broggini N, Jensen SS, Bornstein MM, Schenk RK, Buser D. Membrane durability and tissue response of different bioresorbable barrier membranes: a histologic study in the rabbit calvarium. Int J Oral Maxillofac Implants. 2005; 20: 843-53.