한국발생생물학회지:발생과생식 (Development and Reproduction)

  • 제26권2호
  • /
  • Pages.79-90
  • /
  • 2022
  • /
  • 2465-9525(pISSN)
  • /
  • 2465-9541(eISSN)
한국발생생물학회 (The Korean Society of Developmental Biology)
권호 표지
DOI QR코드

DOI QR Code

Zebrafish Klf11b is Required to Maintain Cell Viability by Inhibiting p53-Mediated Apoptosis

  • Kong, Hee Jeong (Biotechnology Research Division, National Institute of Fisheries Science) ;
  • Lee, Jung Jin (Dept. of Chemical and Biological Engineering, Hanbat National University) ;
  • Kim, Ju-Won (Biotechnology Research Division, National Institute of Fisheries Science) ;
  • Kim, Julan (Biotechnology Research Division, National Institute of Fisheries Science) ;
  • Kim, Young-Ok (Biotechnology Research Division, National Institute of Fisheries Science) ;
  • Yeo, Sang-Yeob (Dept. of Chemical and Biological Engineering, Hanbat National University)
  • 투고 : 2022.03.18
  • 심사 : 2022.06.03
  • 발행 : 2022.06.30
PDF 다운로드
⟨ 이전 논문 다음 논문 ⟩

초록

Krüppel-like factor 10 (KLF10) regulates various cellular functions, such as proliferation, differentiation and apoptosis, as well as the homeostasis of several types of tissue. In the present study, we attempted a loss-of-function analysis of zebrafish Klf11a and Klf11b, which constitute human KLF10 homologs. Embryos injected with klf11b-morpholino (MO) showed developmental retardation and cell death, whereas klf11a-MO-injected embryos showed normal development. In klf11b-MO-injected embryos, a dramatic increase in the amount of zebrafish p53 mRNA might be the cause of the increase in that of bax. The degree of apoptosis decreased in the klf11b-MO and p53-MO co-injected embryos. These findings imply that KLF10 is a negative regulator of p53-dependent transcription, suggesting that the KLF10/p53 complex may play an important role in apoptosis for maintenance of tissue homeostasis during embryonic development.

키워드

과제정보

We thank the members of Yeo and Kong's Lab. This research was supported by a grant from the National Institute of Fisheries Science (NIFS), Korea (R2022027).

참고문헌

  1. Black AR, Black JD, Azizkhan-Clifford J (2001) Sp1 and kruppel-like factor family of transcription factors in cell growth regulation and cancer. J Cell Physiol 188:143-160. https://doi.org/10.1002/jcp.1111
  2. Cook T, Gebelein B, Mesa K, Mladek A, Urrutia R (1998) Molecular cloning and characterization of TIEG2 reveals a new subfamily of transforming growth factor-β-inducible Sp1-like zinc finger-encoding genes involved in the regulation of cell growth. J Biol Chem 273:25929-25936. https://doi.org/10.1074/jbc.273.40.25929
  3. Fernandez-Zapico ME, Mladek A, Ellenrieder V, Folch-Puy E, Miller L, Urrutia R (2003) An mSin3A interaction domain links the transcriptional activity of KLF11 with its role in growth regulation. EMBO J 22:4748-4758. https://doi.org/10.1093/emboj/cdg470
  4. Hendzel MJ, Wei Y, Mancini MA, Van Hooser A, Ranalli T, Brinkley BR, Bazett-Jones DP, Allis CD (1997) Mitosis-specific phosphorylation of histone H3 initiates primarily within pericentromeric heterochromatin during G2 and spreads in an ordered fashion coincident with mitotic chromosome condensation. Chromosoma 106:348-360. https://doi.org/10.1007/s004120050256
  5. Heo SH, Jeong ES, Lee KS, Seo JH, Lee WK, Choi YK (2015) Kruppel-like factor 10 null mice exhibit lower tumor incidence and suppressed cellular proliferation activity following chemically induced liver tumorigenesis. Oncol Rep 33:2037-2044. https://doi.org/10.3892/or.2015.3801
  6. Higashijima S, Hotta Y, Okamoto H (2000) Visualization of cranial motor neurons in live transgenic zebrafish expressing green fluorescent protein under the control of the islet-1 promoter/enhancer. J Neurosci 20:206-218. https://doi.org/10.1523/jneurosci.20-01-00206.2000
  7. Jin W, Di G, Li J, Chen Y, Li W, Wu J, Cheng T, Yao M, Shao Z (2007) TIEG1 induces apoptosis through mitochondrial apoptotic pathway and promotes apoptosis induced by homoharringtonine and velcade. FEBS Lett 581:3826-3832. https://doi.org/10.1016/j.febslet.2007.07.008
  8. Jung SH, Kim HS, Ryu JH, Gwak JW, Bae YK, Kim CH, Yeo SY (2012) Her4-positive population in the tectum opticum is proliferating neural precursors in the adult zebrafish brain. Mol Cells 33:627-632. https://doi.org/10.1007/s10059-012-0091-5
  9. Kimmel CB, Ballard WW, Kimmel SR, Ullmann B, Schilling TF (1995) Stages of embryonic development of the zebrafish. Dev Dyn 203:253-310. https://doi.org/10.1002/aja.1002030302
  10. Mungamuri SK, Qiao RF, Yao S, Manfredi JJ, Gu W, Aaronson SA (2016) USP7 enforces heterochromatinization of p53 target promoters by protecting SUV39H1 from MDM2- mediated degradation. Cell Rep 14:2528-2537. https://doi.org/10.1016/j.celrep.2016.02.049
  11. Oltval ZN, Milliman CL, Korsmeyer SJ (1993) Bcl-2 heterodimerizes in vivo with a conserved homolog, Bax, that accelerates programmed cell death. Cell 74:609-619. https://doi.org/10.1016/0092-8674(93)90509-o
  12. Ribeiro A, Bronk SF, Roberts PJ, Urrutia R, Gores GJ (1999) The transforming growth factor β1-inducible transcription factor, TIEG1, mediates apoptosis through oxidative stress. Hepatology 30:1490-1497. https://doi.org/10.1002/hep.510300620
  13. Sirach E, Bureau C, Peron JM, Pradayrol L, Vinel JP, Buscail L, Cordelier P (2007) KLF6 transcription factor protects hepatocellular carcinoma-derived cells from apoptosis. Cell Death Differ 14:1202-1210. https://doi.org/10.1038/sj.cdd.4402114
  14. Song KD, Kim DJ, Lee JE, Yun CH, Lee WK (2012) KLF10, transforming growth factor-βinducible early gene 1, acts as a tumor suppressor. Biochem Biophys Res Commun 419:388-394. https://doi.org/10.1016/j.bbrc.2012.02.032
  15. Subramaniam M, Harris SA, Oursler MJ, Rasmussen K, Riggs BL, Spelsberg TC (1995) Identification of a novel TGF-β-regulated gene encoding a putative zinc finger protein in human osteoblasts. Nucleic Acids Res 23:4907-4912. https://doi.org/10.1093/nar/23.23.4907
  16. Subramaniam M, Hawse JR, Rajamannan NM, Ingle JN, Spelsberg TC (2010) Functional role of KLF10 in multiple disease processes. Biofactors 36:8-18.
  17. Tachibana I, Imoto M, Adjei PN, Gores GJ, Subramaniam M, Spelsberg TC, Urrutia R (1997) Overexpression of the TGFbeta-regulated zinc finger encoding gene, TIEG, induces apoptosis in pancreatic epithelial cells. J Clin Invest 99:2365-2374. https://doi.org/10.1172/JCI119418
  18. Xue Y, Gao S, Liu F (2015) Genome-wide analysis of the zebrafish Klf family identifies two genes important for erythroid maturation. Dev Biol 403:115-127. https://doi.org/10.1016/j.ydbio.2015.05.015
  19. Yabu T, Kishi S, Okazaki T, Yamashita M (2001) Characterization of zebrafish caspase-3 and induction of apoptosis through ceramide generation in fish fathead minnow tailbud cells and zebrafish embryo. Biochem J 360:39-47. https://doi.org/10.1042/bj3600039
  20. Yeo SY, Miyashita T, Fricke C, Little MH, Yamada T, Kuwada JY, Huh TL, Chien CB, Okamoto H (2004) Involvement of Islet-2 in the Slit signaling for axonal branching and defasciculation of the sensory neurons in embryonic zebrafish. Mech Dev 121:315-324. https://doi.org/10.1016/j.mod.2004.03.006
  21. Zhang JS, Moncrieffe MC, Kaczynski J, Ellenrieder V, Prendergast FG, Urrutia R (2001) A conserved α-helical motif mediates the interaction of Sp1-like transcriptional repressors with the corepressor mSin3A. Mol Cell Biol 21:5041-5049. https://doi.org/10.1128/MCB.21.15.5041-5049.2001