Clinical and Experimental Reproductive Medicine

The Korean Society for Reproductive Medicine (대한생식의학회)
권호 표지
DOI QR코드

DOI QR Code

High efficiency of homemade culture medium supplemented with GDF9-β in human oocytes for rescue in vitro maturation

  • Mohsenzadeh, Mehdi (Gerash Al-Zahra Fertility Center, Gerash University of Medical Sciences) ;
  • Khalili, Mohammad Ali (Research and Clinical Center for Infertility, Yazd Reproductive Sciences Institute, Shahid Sadoughi University of Medical Sciences) ;
  • Anbari, Fatemeh (Research and Clinical Center for Infertility, Yazd Reproductive Sciences Institute, Shahid Sadoughi University of Medical Sciences) ;
  • Vatanparast, Mahboubeh (Molecular Medicine Research Center, Research Institute of Basic Medical Sciences, Rafsanjan University of Medical Sciences)
  • Received : 2021.10.18
  • Accepted : 2022.03.07
  • Published : 2022.06.30
Download PDF
⟨ Previous Next ⟩

Abstract

Objective: Optimizing culture media for the incubation of immature oocytes is a vital strategy to increase the oocyte maturation rate during in vitro maturation (IVM) programs. This study evaluated the IVM and fertilization rates of human germinal vesicle (GV) and metaphase I (MI) oocytes using two different maturation media (commercial and homemade) with or without growth differentiation factor 9-β (GDF9-β). supplementation. Methods: Immature oocytes from intracytoplasmic sperm injection (ICSI) cycles were collected and assigned to one of two IVM culture media (commercial or homemade; cleavage-stage base). After maturation, MII oocytes were examined under an inverted microscope for the presence of the polar body, zona pellucida (ZP) birefringence, and meiotic spindle (MS) visualization after maturation in four conditions (commercial or homemade medium, with or without GDF9-β. ICSI was done for matured oocytes, and fertilization was confirmed by the visualization of two distinct pronuclei and two polar bodies. Results: No significant differences were found between the two culture media in terms of the time and rate of oocyte maturation or the rate of fertilization (p>0.05). Growth factor supplementation increased the 24-hour maturation rate for both GV and MI oocytes only in homemade medium. The maturation rate after 24 hours was higher for MI oocytes (p<0.05). Similar results were observed for MS visualization and ZP structure in both types of media (p>0.05). Conclusion: Higher rates of oocyte maturation and fertilization were observed after application of homemade medium supplemented with GDF9-β. Therefore, this combination may be recommended as an alternative for clinical IVM programs.

Keywords

References

  1. de Araujo CH, Nogueira D, de Araujo MC, Martins Wde P, Ferriani RA, dos Reis RM. Supplemented tissue culture medium 199 is a better medium for in vitro maturation of oocytes from women with polycystic ovary syndrome women than human tubal fluid. Fertil Steril 2009;91:509-13. https://doi.org/10.1016/j.fertnstert.2007.11.082
  2. Yeo CX, Gilchrist RB, Thompson JG, Lane M. Exogenous growth differentiation factor 9 in oocyte maturation media enhances subsequent embryo development and fetal viability in mice. Hum Reprod 2008;23:67-73. https://doi.org/10.1093/humrep/dem140
  3. Vuong LN, Ho TM, Gilchrist RB, Smitz J. The place of in vitro maturation in assisted reproductive technology. Fertil Reprod 2019;1:11-5. https://doi.org/10.1142/S2661318219300022
  4. Gong X, Li H, Zhao Y. The improvement and clinical application of human oocyte in vitro maturation (IVM). Reprod Sci 2021 Jun 2 [Epub]. https://doi.org/10.1007/s43032-021-00613-3.
  5. Lee HJ, Barad DH, Kushnir VA, Shohat-Tal A, Lazzaroni-Tealdi E, Wu YG, et al. Rescue in vitro maturation (IVM) of immature oocytes in stimulated cycles in women with low functional ovarian reserve (LFOR). Endocrine 2016;52:165-71. https://doi.org/10.1007/s12020-015-0744-1
  6. Rienzi L, Ubaldi F, Anniballo R, Cerulo G, Greco E. Preincubation of human oocytes may improve fertilization and embryo quality after intracytoplasmic sperm injection. Hum Reprod 1998;13:1014-9. https://doi.org/10.1093/humrep/13.4.1014
  7. Huang FJ, Chang SY, Tsai MY, Lin YC, Kung FT, Wu JF, et al. Relationship of the human cumulus-free oocyte maturational profile with in vitro outcome parameters after intracytoplasmic sperm injection. J Assist Reprod Genet 1999;16:483-7. https://doi.org/10.1023/A:1020551000150
  8. Tantitham C, Panunumpa S, Satirapod C. The effect of human chorionic gonadotropin on the in vitro development of immature to mature human oocytes: a randomized controlled study. J Hum Reprod Sci 2020;13:133-7. https://doi.org/10.4103/jhrs.JHRS_1_20
  9. Omidi M, Khalili MA, Ashourzadeh S, Rahimipour M. Zona pellucida birefringence and meiotic spindle visualisation of human oocytes are not influenced by IVM technology. Reprod Fertil Dev 2014;26:407-13. https://doi.org/10.1071/RD13001
  10. Fesahat F, Faramarzi A, Sheikhha MH, Firouzabadi RD, Khalili MA. Comparing the effects of different in vitro maturation media on IVM outcomes of MI oocytes. Middle East Fertil Soc J 2017;22: 174-7. https://doi.org/10.1016/j.mefs.2017.03.002
  11. Filali M, Hesters L, Fanchin R, Tachdjian G, Frydman R, Frydman N. Retrospective comparison of two media for invitro maturation of oocytes. Reprod Biomed Online 2008;16:250-6. https://doi.org/10.1016/S1472-6483(10)60582-2
  12. Izquierdo D, Villamediana P, Paramio MT. Effect of culture media on embryo development from prepubertal goat IVM-IVF oocytes. Theriogenology 1999;52:847-61. https://doi.org/10.1016/S0093-691X(99)00177-6
  13. Faramarzi A, Khalili MA, Ashourzadeh S, Palmerini MG. Does rescue in vitro maturation of germinal vesicle stage oocytes impair embryo morphokinetics development? Zygote 2018;26:430-4. https://doi.org/10.1017/s0967199418000515
  14. Kim M, Hong SJ, Lee JH, Min CK, Hwang KJ, Park RW. Comparison of in vitro maturation media of immature oocytes: the effectiveness of blastocyst culture media. Fertil Steril 2011;95:554-7. https://doi.org/10.1016/j.fertnstert.2010.10.035
  15. Dahan MH, Tan SL, Chung J, Son WY. Clinical definition paper on in vitro maturation of human oocytes. Hum Reprod 2016;31:1383-6. https://doi.org/10.1093/humrep/dew109
  16. Chatroudi MH, Khalili MA, Ashourzadeh S, Anbari F, Shahedi A, Safari S. Growth differentiation factor 9 and cumulus cell supplementation in in vitro maturation culture media enhances the viability of human blastocysts. Clin Exp Reprod Med 2019;46:166-72. https://doi.org/10.5653/cerm.2019.00402
  17. Lin ZL, Li YH, Xu YN, Wang QL, Namgoong S, Cui XS, et al. Effects of growth differentiation factor 9 and bone morphogenetic protein 15 on the in vitro maturation of porcine oocytes. Reprod Domest Anim 2014;49:219-27. https://doi.org/10.1111/rda.12254
  18. Pangas SA, Matzuk MM. The art and artifact of GDF9 activity: cumulus expansion and the cumulus expansion-enabling factor. Biol Reprod 2005;73:582-5. https://doi.org/10.1095/biolreprod.105.042127
  19. Fesahat F, Dehghani Firouzabadi R, Faramarzi A, Khalili MA. The effects of different types of media on in vitro maturation outcomes of human germinal vesicle oocytes retrieved in intracytoplasmic sperm injection cycles. Clin Exp Reprod Med 2017;44:79-84. https://doi.org/10.5653/cerm.2017.44.2.79
  20. Azoury J, Verlhac MH, Dumont J. Actin filaments: key players in the control of asymmetric divisions in mouse oocytes. Biol Cell 2009;101:69-76. https://doi.org/10.1042/BC20080003
  21. Nivet AL, Vigneault C, Blondin P, Sirard MA. Changes in granulosa cells' gene expression associated with increased oocyte competence in bovine. Reproduction 2013;145:555-65. https://doi.org/10.1530/REP-13-0032
  22. Arroyo A, Kim B, Yeh J. Luteinizing hormone action in human oocyte maturation and quality: signaling pathways, regulation, and clinical impact. Reprod Sci 2020;27:1223-52. https://doi.org/10.1007/s43032-019-00137-x
  23. Sanders JR, Jones KT. Regulation of the meiotic divisions of mammalian oocytes and eggs. Biochem Soc Trans 2018;46:797-806. https://doi.org/10.1042/BST20170493
  24. Ashourzadeh S, Khalili MA, Omidi M, Mahani SN, Kalantar SM, Aflatoonian A, et al. Noninvasive assays of in vitro matured human oocytes showed insignificant correlation with fertilization and embryo development. Arch Gynecol Obstet 2015;292:459-63. https://doi.org/10.1007/s00404-015-3644-y
  25. Chen SU, Chen HF, Lien YR, Ho HN, Chang HC, Yang YS. Schedule to inject in vitro matured oocytes may increase pregnancy after intracytoplasmic sperm injection. Arch Androl 2000;44:197-205. https://doi.org/10.1080/014850100262173
  26. Moschini RM, Chuang L, Poleshchuk F, Slifkin RE, Copperman AB, Barritt J. Commercially available enhanced in vitro maturation medium does not improve maturation of germinal vesicle and metaphase I oocytes in standard in vitro fertilization cases. Fertil Steril 2011;95:2645-7. https://doi.org/10.1016/j.fertnstert.2011.03.094
  27. Jurema MW, Nogueira D. In vitro maturation of human oocytes for assisted reproduction. Fertil Steril 2006;86:1277-91. https://doi.org/10.1016/j.fertnstert.2006.02.126
  28. Edirisinghe WR, Junk SM, Matson PL, Yovich JL. Birth from cryopreserved embryos following in-vitro maturation of oocytes and intracytoplasmic sperm injection. Hum Reprod 1997;12:1056-8. https://doi.org/10.1093/humrep/12.5.1056
  29. Edwards RG. Maturation in vitro of human ovarian oocytes. Lancet 1965;2:926-9. https://doi.org/10.1016/S0140-6736(65)92903-X
  30. Son WY, Lee SY, Lim JH. Fertilization, cleavage and blastocyst development according to the maturation timing of oocytes in in vitro maturation cycles. Hum Reprod 2005;20:3204-7. https://doi.org/10.1093/humrep/dei195
  31. Hussein TS, Thompson JG, Gilchrist RB. Oocyte-secreted factors enhance oocyte developmental competence. Dev Biol 2006;296: 514-21. https://doi.org/10.1016/j.ydbio.2006.06.026
  32. Prochowska S, Nizanski W, Partyka A, Kochan J, Mlodawska W, Nowak A, et al. The use of human and bovine commercial media for oocyte maturation and embryo development in the domestic cat (Felis catus). Reprod Domest Anim 2019;54:719-26. https://doi.org/10.1111/rda.13418
  33. Gook DA, Edgar DH, Borg J, Martic M. Detection of zona pellucida proteins during human folliculogenesis. Hum Reprod 2008;23: 394-402. https://doi.org/10.1093/humrep/dem373
  34. Villamediana P, Ruttlant J, opez-B ML, Vidal F, Paramio M. Changes in zona pellucida surface after in vivo and in vitro maturation of caprine oocytes. Reprod Domest Anim 1999;34:417-21. https://doi.org/10.1046/j.1439-0531.1999.00179.x
  35. Wang WH, Keefe DL. Prediction of chromosome misalignment among in vitro matured human oocytes by spindle imaging with the PolScope. Fertil Steril 2002;78:1077-81. https://doi.org/10.1016/S0015-0282(02)04196-1
  36. Braga DP, Figueira Rde C, Rodrigues D, Madaschi C, Pasqualotto FF, Iaconelli A Jr, et al. Prognostic value of meiotic spindle imaging on fertilization rate and embryo development in in vitro-matured human oocytes. Fertil Steril 2008;90:429-33. https://doi.org/10.1016/j.fertnstert.2007.06.088
  37. Wang WH, Meng L, Hackett RJ, Keefe DL. Developmental ability of human oocytes with or without birefringent spindles imaged by Polscope before insemination. Hum Reprod 2001;16:1464-8. https://doi.org/10.1093/humrep/16.7.1464
  38. Rienzi L, Ubaldi F, Iacobelli M, Minasi MG, Romano S, Greco E. Meiotic spindle visualization in living human oocytes. Reprod Biomed Online 2005;10:192-8. https://doi.org/10.1016/S1472-6483(10)60940-6
  39. Li Y, Feng HL, Cao YJ, Zheng GJ, Yang Y, Mullen S, et al. Confocal microscopic analysis of the spindle and chromosome configurations of human oocytes matured in vitro. Fertil Steril 2006;85: 827-32. https://doi.org/10.1016/j.fertnstert.2005.06.064
  40. Rama Raju GA, Prakash GJ, Krishna KM, Madan K. Meiotic spindle and zona pellucida characteristics as predictors of embryonic development: a preliminary study using PolScope imaging. Reprod Biomed Online 2007;14:166-74. https://doi.org/10.1016/S1472-6483(10)60784-5
  41. Farsi MM, Kamali N, Pourghasem M. Embryological aspects of oocyte in vitro maturation. Int J Mol Cell Med 2013;2:99-109.
  42. Kim BK, Lee SC, Kim KJ, Han CH, Kim JH. In vitro maturation, fertilization, and development of human germinal vesicle oocytes collected from stimulated cycles. Fertil Steril 2000;74:1153-8. https://doi.org/10.1016/S0015-0282(00)01617-4
  43. Reichman DE, Politch J, Ginsburg ES, Racowsky C. Extended in vitro maturation of immature oocytes from stimulated cycles: an analysis of fertilization potential, embryo development, and reproductive outcomes. J Assist Reprod Genet 2010;27:347-56. https://doi.org/10.1007/s10815-010-9416-5
  44. De Vos M, Grynberg M, Ho TM, Yuan Y, Albertini DF, Gilchrist RB. Perspectives on the development and future of oocyte IVM in clinical practice. J Assist Reprod Genet 2021;38:1265-80. https://doi.org/10.1007/s10815-021-02263-5
  45. Kirillova A, Bunyaeva E, Van Ranst H, Khabas G, Farmakovskaya M, Kamaletdinov N, et al. Improved maturation competence of ovarian tissue oocytes using a biphasic in vitro maturation system for patients with gynecological malignancy: a study on sibling oocytes. J Assist Reprod Genet 2021;38:1331-40. https://doi.org/10.1007/s10815-021-02118-z