Clinical and Experimental Reproductive Medicine

  • 제49권2호
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  • Pages.87-92
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  • 2022
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  • 2233-8233(pISSN)
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  • 2233-8241(eISSN)
대한생식의학회 (The Korean Society for Reproductive Medicine)
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Melatonin and selenium supplementation in extenders improves the post-thaw quality parameters of rat sperm

  • 투고 : 2022.02.01
  • 심사 : 2022.03.14
  • 발행 : 2022.06.30
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초록

Objective: The aim of this study was to determine the effects of melatonin and selenium in freezing extenders on frozen-thawed rat sperm. Methods: Semen samples were collected from 20 adult male Wistar albino rats. Following dilution, the samples were divided into six groups: four cryopreserved groups with 1 mM and 0.5 mM melatonin and selenium supplements, and two fresh and cryopreserved control groups. The rapid freezing technique was used to freeze the samples. Flow cytometry was used to assess plasma membrane integrity, mitochondrial membrane potential, and DNA damage, while computer-assisted sperm analysis was used to assess motility. Results: Total motility was higher in the 1 mM melatonin supplementation group than in the cryopreserved control group (mean±standard error of the mean, 69.89±3.05 vs. 59.21±1.31; p≤0.05). The group with 1 mM selenium had the highest plasma membrane integrity (42.35%±1.01%). The cryopreserved group with 0.5 mM selenium had the highest mitochondrial membrane potential, whereas the cryopreserved control group had the lowest (45.92%±4.53% and 39.45%±3.52%, respectively). Conclusion: Cryopreservation of rat semen supplemented with 1 mM melatonin increased sperm motility after freeze-thawing, while supplementation with 0.5 mM selenium increased mitochondrial activity.

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참고문헌

  1. Mehaisen G, Partyka A, Ligocka Z, Nizanski W. Cryoprotective effect of melatonin supplementation on post-thawed rooster sperm quality. Anim Reprod Sci 2020;212:106238. https://doi.org/10.1016/j.anireprosci.2019.106238
  2. Chatterjee S, Gagnon C. Production of reactive oxygen species by spermatozoa undergoing cooling, freezing, and thawing. Mol Reprod Dev 2001;59:451-8. https://doi.org/10.1002/mrd.1052
  3. Rui BR, Shibuya FY, Kawaoku A, Losano J, Angrimani D, Dalmazzo A, et al. Impact of induced levels of specific free radicals and malondialdehyde on chicken semen quality and fertility. Theriogenology 2017;90:11-9. https://doi.org/10.1016/j.theriogenology.2016.11.001
  4. ChaithraShree AR, Ingole SD, Dighe VD, Nagvekar AS, Bharucha SV, Dagli NR, et al. Effect of melatonin on bovine sperm characteristics and ultrastructure changes following cryopreservation. Vet Med Sci 2020;6:177-86. https://doi.org/10.1002/vms3.224
  5. Gomes Sanches E, Antonio Moreira Tosta G, eronimo Souza-Filho J. Economic feasibility of cobia juvenile production (Rachycentron canadum). Bol Inst Pesca 2013;39:15-26.
  6. Boutin JA, Audinot V, Ferry G, Delagrange P. Molecular tools to study melatonin pathways and actions. Trends Pharmacol Sci 2005;26:412-9. https://doi.org/10.1016/j.tips.2005.06.006
  7. Tan DX, Reiter RJ, Manchester LC, Yan MT, El-Sawi M, Sainz RM, et al. Chemical and physical properties and potential mechanisms: melatonin as a broad spectrum antioxidant and free radical scavenger. Curr Top Med Chem 2002;2:181-97. https://doi.org/10.2174/1568026023394443
  8. Carrasco C, Rodriguez AB, Pariente JA. Melatonin as a stabilizer of mitochondrial function: role in diseases and aging. Turk J Biol 2015;39:822-31. https://doi.org/10.3906/biy-1504-26
  9. Reiter RJ. Melatonin: lowering the high price of free radicals. News Physiol Sci 2000;15:246-50.
  10. Bas E, Naziroglu M. Treatment with melatonin and selenium attenuates docetaxel-induced apoptosis and oxidative injury in kidney and testes of mice. Andrologia 2019;51:e13320.
  11. Lopes JT, Salmito-Vanderley CS, Almeida-Monteiro PS. Presenca de antioxidantes no semen de teleosteos e sua utilizacao na suplementacao de meios de congelacao seminal. R Bras Reprod Anim 2016;40:29-34.
  12. Burk RF. Selenium, an antioxidant nutrient. Nutr Clin Care 2002;5: 75-9. https://doi.org/10.1046/j.1523-5408.2002.00006.x
  13. Lemoine M, Mignon-Grasteau S, Grasseau I, Magistrini M, Blesbois E. Ability of chicken spermatozoa to undergo acrosome reaction after liquid storage or cryopreservation. Theriogenology 2011; 75:122-30. https://doi.org/10.1016/j.theriogenology.2010.07.017
  14. Wani NA, Hong S. Intracytoplasmic sperm injection (ICSI) of in vitro matured oocytes with stored epididymal spermatozoa in camel (Camelus dromedarius): effect of exogenous activation on in vitro embryo development. Theriogenology 2018;113:44-9. https://doi.org/10.1016/j.theriogenology.2018.02.004
  15. Ali A, Derar DR, Alhassun TM, Almundarij TI. Effect of zinc, selenium, and vitamin E administration on semen quality and fertility of male dromedary camels with impotentia generandi. Biol Trace Elem Res 2021;199:1370-6. https://doi.org/10.1007/s12011-020-02276-8
  16. Ahmadvand H, Babaeenezhad E, Nayeri H, Nezhad ZZ. Selenium effects on antioxidant and inflammatory indices in renal ischemia-reperfusion injury in rats. J Renal Inj Prev 2018;8:71-7. https://doi.org/10.15171/jrip.2019.14
  17. Varisli O, Scott H, Agca C, Agca Y. The effects of cooling rates and type of freezing extenders on cryosurvival of rat sperm. Cryobiology 2013;67:109-16. https://doi.org/10.1016/j.cryobiol.2013.05.009
  18. Fadl AM, Ghallab A, Abou-Ahmed MM, Moawad AR. Melatonin can improve viability and functional integrity of cooled and frozen/thawed rabbit spermatozoa. Reprod Domest Anim 2021;56:103-11. https://doi.org/10.1111/rda.13853
  19. Khalil WA, El-Harairy MA, Zeidan A, Hassan M. Impact of selenium nano-particles in semen extender on bull sperm quality after cryopreservation. Theriogenology 2019;126:121-7. https://doi.org/10.1016/j.theriogenology.2018.12.017
  20. Soleimanzadeh A, Saberivand A. Effect of curcumin on rat sperm morphology after the freeze-thawing process. Vet Res Forum 2013;4:185-9.
  21. Koohestanidehaghi Y, Torkamanpari M, Shirmohamadi Z, Lorian K, Vatankhah M. The effect of cysteine and glutamine on human sperm functional parameters during vitrification. Andrologia 2021;53:e13870.
  22. Ghorbani F, Nasiri Z, Koohestanidehaghi Y, Lorian K. The antioxidant roles of L-carnitine and N-acetyl cysteine against oxidative stress on human sperm functional parameters during vitrification. Clin Exp Reprod Med 2021;48:316-21. https://doi.org/10.5653/cerm.2021.04560
  23. Gungor S, Inanc ME, Ozturk C, Korkmaz F, Bastan I, Cil B, et al. Gallic and carnosic acids improve quality of frozen-thawed ram spermatozoa. Andrologia 2019;51:e13393.
  24. Medrano A, Contreras CF, Herrera FM, Alcantar-Rodriguez AM. Melatonin as an antioxidant preserving sperm from domestic animals. Asian Pac J Reprod 2017;6:241. https://doi.org/10.4103/2305-0500.217317
  25. Banday MN, Lone FA, Rasool F, Rashid M, Shikari A. Use of antioxidants reduce lipid peroxidation and improve quality of crossbred ram sperm during its cryopreservation. Cryobiology 2017;74:25-30. https://doi.org/10.1016/j.cryobiol.2016.12.008
  26. El-Raey M, Badr MR, Rawash ZM, Darwish GM. Evidences for the role of melatonin as a protective additive during buffalo semen freezing. Am J Anim Vet Sci 2014;9:252-62. https://doi.org/10.3844/ajavsp.2014.252.262
  27. Fang Y, Zhao C, Xiang H, Jia G, Zhong R. Melatonin improves cryopreservation of ram sperm by inhibiting mitochondrial permeability transition pore opening. Reprod Domest Anim 2020;55: 1240-9. https://doi.org/10.1111/rda.13771
  28. Feng TY, Li Q, Ren F, Xi HM, Lv DL, Li Y, et al. Melatonin protects goat spermatogonial stem cells against oxidative damage during cryopreservation by improving antioxidant capacity and inhibiting mitochondrial apoptosis pathway. Oxid Med Cell Longev 2020;2020:5954635.
  29. He B, Guo H, Gong Y, Zhao R. Lipopolysaccharide-induced mitochondrial dysfunction in boar sperm is mediated by activation of oxidative phosphorylation. Theriogenology 2017;87:1-8. https://doi.org/10.1016/j.theriogenology.2016.07.030
  30. Ugur MR, Saber Abdelrahman A, Evans HC, Gilmore AA, Hitit M, Arifiantini RI, et al. Advances in cryopreservation of bull sperm. Front Vet Sci 2019;6:268. https://doi.org/10.3389/fvets.2019.00268
  31. Pool KR, Rickard JP, Tumeth E, de Graaf SP. Treatment of rams with melatonin implants in the non-breeding season improves post-thaw sperm progressive motility and DNA integrity. Anim Reprod Sci 2020;221:106579. https://doi.org/10.1016/j.anireprosci.2020.106579
  32. Rezaeian Z, Yazdekhasti H, Nasri S, Rajabi Z, Fallahi P, Amidi F. Effect of selenium on human sperm parameters after freezing and thawing procedures. Asian Pac J Reprod 2016;5:462-6. https://doi.org/10.1016/j.apjr.2016.11.001
  33. Browne RK, Kaurova SA, Uteshev VK, Shishova NV, McGinnity D, Figiel CR, et al. Sperm motility of externally fertilizing fish and amphibians. Theriogenology 2015;83:1-13. https://doi.org/10.1016/j.theriogenology.2014.09.018
  34. World Health Organization. WHO laboratory manual for the examination and processing of human semen. Geneva: World Health Organization; 2010.
  35. Rateb SA, Khalifa MA, Abd El-Hamid IS, Shedeed HA. Enhancing liquid-chilled storage and cryopreservation capacities of ram spermatozoa by supplementing the diluent with different additives. Asian-Australas J Anim Sci 2020;33:1068-76. https://doi.org/10.5713/ajas.19.0338
  36. Breininger E, Beorlegui NB, O'Flaherty CM, Beconi MT. Alpha-tocopherol improves biochemical and dynamic parameters in cryopreserved boar semen. Theriogenology 2005;63:2126-35. https://doi.org/10.1016/j.theriogenology.2004.08.016