대한본초학회지 (The Korea Journal of Herbology)

  • 제37권4호
  • /
  • Pages.1-8
  • /
  • 2022
  • /
  • 1229-1765(pISSN)
  • /
  • 2288-7199(eISSN)
대한본초학회 (The Korea Association of Herbology)
권호 표지
DOI QR코드

DOI QR Code

산약(山藥) 물 추출물의 급성 췌장염 보호 효과

Protective effects of Dioscorea batas Decaisne water extract on acute pancreatitis

  • 권빛나 (원광대학교 한의과대학 약리학교실) ;
  • 배기상 (원광대학교 한의과대학 약리학교실)
  • Kweon, Bitna (Department of Pharmacology, School of Korean Medicine, Wonkwang University) ;
  • Bae, Gi-Sang (Department of Pharmacology, School of Korean Medicine, Wonkwang University)
  • 투고 : 2022.06.14
  • 심사 : 2022.07.25
  • 발행 : 2022.07.30
PDF 다운로드
⟨ 이전 논문 다음 논문 ⟩

초록

Objectives : Dioscorea batas Decaisne (DB) has been known to be good for the digestive system on Eastern Asia. However, the protective effect of DB on acute pancreatitis (AP) has not been studied. In this study, we tried to investigate the protective effect of DB water extract on caerulein-induced AP. Methods : To measure the protective effect of DB on AP, Mice were injected with cholecystokinin analogue caerulein (50 ㎍/kg) hourly for 6 times. DB water extract (200 or 400 mg/kg) or saline (control group) was administered orally 1 h before the first injection of caerulein. The mice were sacrificed at 6 h after the last injection of caerulein. The pancreas tissues and serum samples were immediately taken for further analysis. Results : Administration of DB water extract showed the inhibitory effect on the increase of pancreas weight/body weight ratio, pancreatic histological damage. And the rise of serum lipase level was significantly reduced in DB water extract treatment group during AP in mice. However administration of DB water extract did not show significant reduction in serum amylase level. Also, mRNA levels of pro-inflammatory cytokines Interleukin (IL)-6 and Tumor necrosis factor (TNF)-𝛼 but not IL-1𝛽 were inhibited by administration of DB water extract. Conclusions : Taken together, we found that administration of DB water extract ameliorates the severity of caerulein-induced AP, which suggests the potential to be an effective treatment on AP.

키워드

과제정보

이 논문은 2019년 정부(교육부)의 재원으로 한국연구재단의지원을 받아 수행된 연구임(한국연구재단-2019-글로벌박사양성사업-2019H1A2A1076626). 이 성과는 정부(교육부/과학기술정보통신부)의 재원으로 한국연구재단의 지원을 받아 수행된 연구임(No. 2019R1A2C1090032/2021R1I1A2053285).

참고문헌

  1. Collaborators GBDC. The global, regional, and national burden of cirrhosis by cause in 195 countries and territories, 1990-2017: a systematic analysis for the Global Burden of Disease Study 2017. Lancet Gastroenterol Hepatol. 2020;5(3):245-66. https://doi.org/10.1016/S2468-1253(19)30349-8
  2. Petrov MS, Shanbhag S, Chakraborty M, Phillips AR, Windsor JA. Organ failure and infection of pancreatic necrosis as determinants of mortality in patients with acute pancreatitis. Gastroenterology. 2010;139(3):813-20. https://doi.org/10.1053/j.gastro.2010.06.010
  3. Global Burdens of Disease Study 2013 Collaborators. Global, regional, and national incidence, prevalence, and years lived with disability for 301 acute and chronic diseases and injuries in 188 countries, 1990 -2013: a systematic analysis for the Global Burden of Disease Study 2013. The lancet. 2015;386(9995): 743-800. https://doi.org/10.1016/S0140-6736(15)60692-4
  4. Weiss FU, Laemmerhirt F, Lerch MM. Etiology and Risk Factors of Acute and Chronic Pancreatitis. Visc Med. 2019;35(2):73-81. https://doi.org/10.1159/000499138
  5. Lee JK. Recent Advances in Management of Acute Pancreatitis. The Korean Journal of Gastroenterology. 2015;66(3):135-43. https://doi.org/10.4166/kjg.2015.66.3.135
  6. Nanjing University of Chinese Medicine. Oriental medicine dictionary. Shanghai: SSTLPH. 2006.217
  7. Kim MJ, Park H-J, Kim KJ, Lee JA, Shin M-R, Roh S-S. Protective Effect of Dioscoreae Rhizoma Extracts in MIA-induced Rat. The Korea Journal of Herbology. 2019;34(4):27-35. https://doi.org/10.6116/KJH.2019.34.4.27
  8. Jeon JR, Lee JS, Lee CH, Kim JY, Kim SD, Nam DH. Effect of ethanol extract of dried Chinese yam (Dioscorea batatas) flour containing dioscin on gastrointestinal function in rat model. Arch Pharm Res. 2006;29(5):348-53. https://doi.org/10.1007/BF02968583
  9. Hou WC, Hsu FL, Lee MH. Yam (Dioscorea batatas) tuber mucilage exhibited antioxidant activities in vitro. Planta Med. 2002;68(12):1072-6. https://doi.org/10.1055/s-2002-36356
  10. Lee S-Y, Yoo D-H, Joo D-H, Lee J-Y. Inhibitory Efficacy of Dioscoreae Rhizoma on MITF, TRP-1, TRP-2, Tyrosinase, PKA and ERK Expression in Melanoma Cells (B16F10). The Korea Journal of Herbology. 2015;30(4):95-100. https://doi.org/10.6116/KJH.2015.30.4.95.
  11. Li CL, Jiang M, Pan CQ, Li J, Xu LG. The global, regional, and national burden of acute pancreatitis in 204 countries and territories, 1990-2019. BMC Gastroenterol. 2021;21(1):332. https://doi.org/10.1186/s12876-021-01906-2
  12. Kirkegard J, Cronin-Fenton D, Heide-Jorgensen U, Mortensen FV. Acute Pancreatitis and Pancreatic Cancer Risk: A Nationwide Matched-Cohort Study in Denmark. Gastroenterology. 2018;154(6):1729-36. https://doi.org/10.1053/j.gastro.2018.02.011
  13. Rijkers AP, Bakker OJ, Ahmed Ali U, Hagenaars J, van Santvoort HC, Besselink MG, Bollen TL, van Eijck CH, Risk of Pancreatic Cancer After a Primary Episode of Acute Pancreatitis. Pancreas. 2017;46(8):1018-22. https://doi.org/10.1097/MPA.0000000000000879
  14. Ahmed Ali U, Issa Y, Hagenaars JC, Bakker OJ, van Goor H, Nieuwenhuijs VB, Bollen TL, van Ramshorst B, Witteman BJ, Brink MA, Schaapherder AF, Dejong CH, Marcel Spanier B.W. Heisterkamp J, van der Harst E, van Eijck CH, Basselink MG, Gooszen HG, van Santvoort HC, Boermeester MA. Risk of Recurrent Pancreatitis and Progression to Chronic Pancreatitis After a First Episode of Acute Pancreatitis. Clin Gastroenterol Hepatol. 2016;14(5): 738-46 https://doi.org/10.1016/j.cgh.2015.12.040
  15. Kim SC, Yang HR. Clinical efficacy of gabexate mesilate for acute pancreatitis in children. European journal of pediatrics. 2013;172(11):1483-90. https://doi.org/10.1007/s00431-013-2068-6
  16. Kwon D-Y,Bu Y-m, Seo B-I, Oh M-S, Lee J-H, Choi H-Y. Herbology. Seoul: Younlimsa. 2004:814-7.
  17. Hu F, Lou N, Jiao J, Guo F, Xiang H, Shang D. Macrophages in pancreatitis: Mechanisms and therapeutic potential. Biomed Pharmacother. 2020; 131:110693. https://doi.org/10.1016/j.biopha.2020.110693
  18. Zhang H, Cai D, Bai X. Macrophages regulate the progression of osteoarthritis. Osteoarthritis and cartilage. 2020;28(5):555-61. https://doi.org/10.1016/j.joca.2020.01.007
  19. Choi G-y, Kim B-w. Experimental study on the antioxidant and antimicrobial properties of Dioscoreae Rhizoma. The Journal of Internal Korean Medicine. 2010;31(2):290-7.
  20. Kim S, Shin S, Park Y, Lee S, Lee C-K, Kim K. Anti-inflammatory activity of Dioscoreae rhizome in macrophages by inhibition of pro-inflammatory cytokines via NF-κB signaling pathway. Am Assoc Immnol; 2011.186(1 Suppplement)54.11.
  21. Lim S-W, Lee S-H, Hur J-M, Lee Y-M, Kim D-K. The Inhibitory Effect of Fermented Dioscoreae batatas Extract on Lipopolysaccharide-induced Macrophage Activation. Yakhak Hoeji. 2011;55(5): 404-10.
  22. Liu L, Guo H, Song A, Huang J, Zhang Y, Jin S, Li s, Zhang L, Yang C, Yang P. Progranulin inhibits LPS-induced macrophage M1 polarization via NF-small ka, CyrillicB and MAPK pathways. BMC Immunol. 2020;21(1):32. https://doi.org/10.1186/s12865-020-00355-y
  23. van Acker GJ, Perides G, Steer ML. Co-localization hypothesis: a mechanism for the intrapancreatic activation of digestive enzymes during the early phases of acute pancreatitis. World J Gastroenterol. 2006;12(13):1985-90. https://doi.org/10.3748/wjg.v12.i13.1985
  24. Bhatia M, Brady M, Shokuhi S, Christmas S, Neoptolemos JP, Slavin J. Inflammatory mediators in acute pancreatitis. J Pathol. 2000;190(2):117-25. https://doi.org/10.1002/(SICI)1096-9896(200002)190:2<117::AID-PATH494>3.0.CO;2-K
  25. Saluja A, Dudeja V, Dawra R, Sah RP. Early Intra-Acinar Events in Pathogenesis of Pancreatitis. Gastroenterology. 2019;156(7):1979-93. https://doi.org/10.1053/j.gastro.2019.01.268
  26. Talukdar R, Sareen A, Zhu H, Yuan Z, Dixit A, Cheema H, George J, Barlass U, Sah R, Garg SK, Banergee S, Garg P, Dudeja V, Dawra R, Saluja AK. Release of Cathepsin B in Cytosol Causes Cell Death in Acute Pancreatitis. Gastroenterology. 2016;151(4):747-58 e5. https://doi.org/10.1053/j.gastro.2016.06.042
  27. Granger J, Remick D. Acute pancreatitis: models, markers, and mediators. Shock. 2005;24 Suppl 1:45-51. https://doi.org/10.1097/01.shk.0000191413.94461.b0
  28. Kim D-U, Bae G-S, Choi J-W, Kim D-G, Kim M-J, Song H-J, Park S-J. Protective effects of Dictamni Radicis Cortex water extract on acute pancreatitis. The Korea Journal of Herbology. 2019;34(1):75-80. https://doi.org/10.6116/KJH.2019.34.1.75
  29. McNally PR. GI/Liver secrets plus E-Book. London : Elsevier Health Sciences; 2010:266
  30. Lee T. Diagnosis and Treatment of Acute Pancreatitis. The Korean Journal of Medicine. 2015;89(5): 494-506. https://doi.org/10.3904/kjm.2015.89.5.494
  31. Koh DH, Kim JH, Lee J, Choi HS. Clinical practice guidelines for acute pancreatitis: the diagnosis of acute pancreatitis. The Korean Journal of Pancreas and Biliary Tract. 2013;18(2):4-13. https://doi.org/10.15279/kpba.2013.18.2.4
  32. Osman MO, Jensen SL. Acute pancreatitis: the pathophysiological role of cytokines and integrins. New trends for treatment? Dig Surg. 1999;16(5): 347-62. https://doi.org/10.1159/000018746
  33. Gross V, Leser HG, Heinisch A, Scholmerich J. Inflammatory mediators and cytokines--new aspects of the pathophysiology and assessment of severity of acute pancreatitis? Hepatogastroenterology. 1993;40(6):522-30.
  34. Kusske AM, Rongione AJ, Reber HA. Cytokines and acute pancreatitis. Gastroenterology. 1996; 110(2):639-42. https://doi.org/10.1053/gast.1996.v110.agast960639
  35. Do JH. Mechanism of severe acute pancreatitis: focusing on development and progression. Korean Journal of Pancreas and Biliary Tract. 2015;20(3): 115-23. https://doi.org/10.15279/kpba.2015.20.3.115
  36. Dugernier TL, Laterre PF, Wittebole X, Roeseler J, Latinne D, Reynaert MS, Pugin J. Compartmentalization of the inflammatory response during acute pancreatitis: correlation with local and systemic complications. Am J Respir Crit Care Med. 2003;168(2):148-57. https://doi.org/10.1164/rccm.2204019
  37. Rao SA, Kunte AR. Interleukin-6: An Early Predictive Marker for Severity of Acute Pancreatitis. Indian J Crit Care Med. 2017;21(7):424-8. https://doi.org/10.4103/ijccm.IJCCM_478_16
  38. Sathyanarayan G, Garg PK, Prasad H, Tandon RK. Elevated level of interleukin-6 predicts organ failure and severe disease in patients with acute pancreatitis. J Gastroenterol Hepatol. 2007;22(4): 550-4. https://doi.org/10.1111/j.1440-1746.2006.04752.x
  39. Denham W, Yang J, Fink G, Denham D, Carter G, Ward K, Norman J. Gene targeting demonstrates additive detrimental effects of interleukin 1 and tumor necrosis factor during pancreatitis. Gastroenterology. 1997;113(5):1741-6. https://doi.org/10.1053/gast.1997.v113.pm9352880
  40. Malleo G, Mazzon E, Siriwardena AK, Cuzzocrea S. Role of tumor necrosis factor-alpha in acute pancreatitis: from biological basis to clinical evidence. Shock. 2007;28(2):130-40. https://doi.org/10.1097/shk.0b013e3180487ba1
  41. Virlos I, Mazzon E, Serraino I, Genovese T, Di Paola R, Thiemerman C, Siriwardena A, Cuzzocrea S. Calpain I inhibitor ameliorates the indices of disease severity in a murine model of cerulein-induced acute pancreatitis. Intensive Care Med. 2004;30(8):1645-51. https://doi.org/10.1007/s00134-004-2328-z
  42. Wagner AC, Mazzucchelli L, Miller M, Camoratto AM, Goke B. CEP-1347 inhibits caerulein-induced rat pancreatic JNK activation and ameliorates caerulein pancreatitis. Am J Physiol Gastrointest Liver Physiol. 2000;278(1):G165-72. https://doi.org/10.1152/ajpgi.2000.278.1.G165
  43. de Beaux AC, Ross JA, Maingay JP, Fearon KC, Carter DC. Proinflammatory cytokine release by peripheral blood mononuclear cells from patients with acute pancreatitis. Br J Surg. 1996;83(8): 1071-5. https://doi.org/10.1002/bjs.1800830811