The Korea Journal of Herbology (대한본초학회지)

The Korea Association of Herbology (대한본초학회)
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Development SCAR marker for the rapid authenticaton of Sinomeni Caulis et Rhizoma based on ITS Sequences

ITS 염기서열 기반 방기 신속 감별용 SCAR marker 개발

  • Kim, Wook Jin (Herbal Medicine Resources Research Center, Korea Institute of Oriental Medicine) ;
  • Noh, Sumin (Herbal Medicine Resources Research Center, Korea Institute of Oriental Medicine) ;
  • Choi, Goya (Herbal Medicine Resources Research Center, Korea Institute of Oriental Medicine) ;
  • Moon, Byeong Cheol (Herbal Medicine Resources Research Center, Korea Institute of Oriental Medicine)
  • 김욱진 (한국한의학연구원 한약자원연구센터) ;
  • 노수민 (한국한의학연구원 한약자원연구센터) ;
  • 최고야 (한국한의학연구원 한약자원연구센터) ;
  • 문병철 (한국한의학연구원 한약자원연구센터)
  • Received : 2022.05.02
  • Accepted : 2022.07.25
  • Published : 2022.07.30
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Abstract

Objectives : In the Korean Pharmacopoeia 12th edition (KP 12) and the Korean Herbal Pharmacopoeia (KHP), two authentic herbal medicines are described, namely Bang-gi (Cheong-pung-deung) and Mok-bang-gi, respectively. In China, Bun-bang-gi is also used as herbal medicine. This study was conducted to develop a molecular authentication tool for distinguishing the three herbal medicine used as Bang-gi, which are Sinomeni Caulis et Rhizoma (Rhizome of Sinomenium acutum), Stephaniae Tetrandrae Radix (Root of Stephania terandra), and Cocculi Radix (Root of Cocculus trilobus). Methods : Twelve samples of three species (four samples of S. acutum, five samples of S. tetrandra, and three samples of C. trilobus) were collected from different habitats. The sequences of internal transcribed spacer (ITS) regions were obtained and comparatively analyzed to design the species-specific sequence characterized amplified region (SCAR) primers. The specificity of each pair of SCAR primers that amplified species-specific amplicon was evaluated for establishing the singleplex and multiplex PCR assay tools. Results : The singleplex SCAR markers show discriminability in C. acutum, S. tetrandra, and C. trilobus. These SCAR markers were also efficiently authenticated three species in the multiplex SCAR amplification using single PCR reaction. Furthermore, these PCR assay methods were applicable to authenticate dried herbal medicines distributed in the markets. Conclusions : The SCAR markers and PCR assay tools help discriminate the three herbal medicines used as Bang-gi at the species levels and provide a reliable genetic method to prevent the inauthentic distribution of these herbal medicines.

Keywords

Acknowledgement

본 연구는 한국한의학연구원의 '지속가능한 한약표준자원 활용기술 개발' 과제(KSN2021320)의 지원으로 수행되었으며, 한국한의학연구원 한약표준표본관 그리고 시료의 분류·동정에 도움을 주신 분류·동정자문위원님께 감사드립니다.

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