Korean journal of applied entomology (한국응용곤충학회지)

Korean Society of Applied Entomology (한국응용곤충학회)
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Repellent Activity of Five Different Plant Extracts Against Aedes albopictus

흰줄숲모기에 대한 5종 식물추출물의 기피효능 평가

  • Park, Seong Ik (Gyeongsangbuk-do Government Public Institute of Health & Environment) ;
  • Park, Jeong Ju (Gyeongsangbuk-do Government Public Institute of Health & Environment) ;
  • Kim, Dan Bi (Gyeongsangbuk-do Government Public Institute of Health & Environment) ;
  • Moon, Na Young (Gyeongsangbuk-do Government Public Institute of Health & Environment) ;
  • Shin, Ji Yoon (Gyeongsangbuk-do Government Public Institute of Health & Environment) ;
  • Cha, Sung Kyung (Gyeongsangbuk-do Government Public Institute of Health & Environment) ;
  • Kim, Sa Ra (Gyeongsangbuk-do Government Public Institute of Health & Environment) ;
  • Kim, Jong-Sik (Department of Biological Sciences, Andong National University) ;
  • Shon, Dong Cheol (Gyeongsangbuk-do Government Public Institute of Health & Environment)
  • 박성익 (경상북도 보건환경연구원) ;
  • 박정주 (경상북도 보건환경연구원) ;
  • 김단비 (경상북도 보건환경연구원) ;
  • 문나영 (경상북도 보건환경연구원) ;
  • 신지윤 (경상북도 보건환경연구원) ;
  • 차성경 (경상북도 보건환경연구원) ;
  • 김사라 (경상북도 보건환경연구원) ;
  • 김종식 (국립 안동대학교 생명과학과) ;
  • 손동철 (경상북도 보건환경연구원)
  • Received : 2022.01.04
  • Accepted : 2022.05.24
  • Published : 2022.06.01
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Abstract

Five different methanol extracts of plants were evaluated as a mosquito repellent against Aedes albopictus in nude mice model. The volatile components of plants were analyzed with GC-MS. The effective dose was the highest in Nepeta cataria with 72.9, 83.7, 86.4, and 97.3% efficiencies at 10, 50, 100, and 200 ㎍/mL, respectively. Nepeta cataria, Actinidia polygama, and Artemisia annua extracts were the most effective in duration time test. And there was no difference compared to negative control in the low mosquito repellent efficiency from 30 minutes. As a result of analyzing the volatile components of each plant, a total of 28 components in Mentha suaveolens, 19 components in Actinidia polygama, 27 components in Artemisia annua, 26 components in Nepeta cataria, and 19 components in Taraxacum platycarpum were detected. Especially, nepetalactone known as an effective ingredient of a mosquito repellent was identified (27.95 mg/Kg) in one of volatile components of Nepeta cataria. Overall, our results provide the possibility to develop mosquito repellents material using natural plants which contain various volatile components.

본 연구에서는, 5가지 식물의 메탄올 추출물을 이용하여 모기기피 효과 실험을 진행하고 누드 쥐를 모델로 하여 흰줄숲모기에 대한 모기기피제의 유효용량 및 유효시간을 평가하였다. 그리고 식물의 휘발성분을 GC-MS로 분석하였다. 유효용량은 개박하가 10, 50, 100, 200 ㎍/mL에서 72.9, 83.7, 86.4, 97.3%의 효율로 가장 높았다. 누드 쥐를 대상으로 한 유효시간 연구에서는 5가지 식물의 메탄올 추출물 중 개박하, 개다래 및 개똥쑥이 90.5%로, 30분까지만 지속하였다. 각 식물의 휘발성분을 분석한 결과, 애플민트 28개 성분, 개다래 19개 성분, 개똥쑥 27개 성분, 개박하 26개 성분, 민들레 19개 성분이 확인 되었다. 특히 모기기피제의 유효 성분으로 알려진 네페탈락톤(nepetalactone)이 개박하의 휘발성분 중 한 성분으로 확인 되었다(27.95 mg/Kg). 본 연구결과는 다양한 휘발성분을 함유한 식물을 이용한 모기기피제 재료로서 가능성을 제시한다.

Keywords

Acknowledgement

본 논문은 경상북도 보건환경연구원 지원에 의해 이루어진 것이며, 논문 교정 및 실험에 도움을 주신 김종식 교수님과 대구한의대학교 정지욱 교수님께 감사의 인사를 드립니다.

References

  1. Birkett, M.A., Hassanali, A., Hoglund, S., Pettersson, J, Pickett, J.A., 2011. Repellent activity of catmint, Nepeta cataria, and iridoid nepetalactone isomers against Afrotropical mosquitoes, ixodid ticks and red poultry mites. Phytochemistry 72, 109-114. https://doi.org/10.1016/j.phytochem.2010.09.016
  2. Choi, H.Y., Yang, Y.C., 2002. Repellent activity of aromatic medicinal plant extracts against Aedes aegypti (Diptera: Culicidae). Korean J. Sanitation 17, 110-114.
  3. Chung, D.H., Lee, W.G., Shin, E.H., 2015. Forecast of Culex tritaeniorhynchus as temperature change. JKDAS 17, 117-125.
  4. Chung, M.H., 2009. Dengue fever. Korean J. Med. 77, 165-170.
  5. Curtis, C.F., Lines, J.D., Lu, B.L., Renz, A., 1990. Natural and synthetic repellents. CRC Press, Inc, Florida, pp. 75-92.
  6. Gkinis, G., Michaelakis, A., Koliopoulos, G., Ioannou, E., Tzakou, O., Roussis, V., 2014. Evaluation of the repellent effects of Nepeta parnassica extract, essential oil, and its major nepetalactone metabolite against mosquitoes. Parasitol. Res. 113, 1127-1134. https://doi.org/10.1007/s00436-013-3750-3
  7. Harbach, R.E., 2007. The Culicidae (Diptera): a review of taxonomy, classification and phylogeny. Zootaxa 1668, 591-638. https://doi.org/10.11646/zootaxa.1668.1.28
  8. Isman, M.B., 1995. Leads and prospects for the development of new botanical inspecticides. Rev. Pestic. Toxicol. 3, 1-20.
  9. Kamimura, K., 1968. The distribution and habitat of medically important mosquitoes of Japan. Jap. J. Sanit. Zool. 19, 15-34. https://doi.org/10.7601/mez.19.15
  10. Kim, D.M., Roh, B.Y., Heo, J.H., Lee, W.G., Yang, S.C., Lee, D.K., 2018. Seasonal prevalence of mosquitoes collected from light traps in Gyeongsangnam province, Republic of Korea (2013-2014). Entomol. Res. 48, 437-445.
  11. Kim, J.K., Kang, C.S., Lee, J.K., Kim, Y.R., Han, H.Y., Yoon, H.K., 2005. Evaluation of repellency effect of two natural aroma mosquito repellent compounds, citronella and citronellal. Entomol. Res. 35, 117-120. https://doi.org/10.1111/j.1748-5967.2005.tb00146.x
  12. Kim, Y.G., Sajjadian, M., Shabbir, A., 2020. Control efficacy of BtPlus against two mosquitoes, Aedes koreicus and Culex vagans. Korean J. Appl. Entomol. 59, 41-54.
  13. Kim, Y.K., Lee, C.M., Lee, J.B., Bae, Y.S., 2012. Seasonal prevalence of mosquitoes and ecological characteristics of anopheline larval occurrence in Gimpo, Gyeonggi province, Republic of Korea. Korean J. Appl. Entomol. 51, 305-312. https://doi.org/10.5656/KSAE.2012.07.0.017
  14. Koren, G., Matsui, D., Bailey, B., 2003. DEET-based insect repellents: safety implications for children and pregnant and lactating women. CMAJ 169, 209-212.
  15. Lee, S.H., 2017. Guidelines for evaluating the effectiveness of mosquito repellents, Ministry of Food and Drug Safety.
  16. Lindsay, L.R., Surgeoner, G.A., Heal, J.D., Gallivan, G.J., 1996. Evaluation of the efficacy of 3% citronella candles and 5% citronella incense for protection against field populations of Aedes mosquitoes. J. AM. Mosq. Control Assoc. 12, 293-294.
  17. Park, M.H., Kim, M.J., Cho, W.I., Chang, P.S., Lee, J.H., 2009. Effect of Treaments on the Distribution of Volatiles in Artemisia prineps Pampan. Korean J. Food Sci. Technol. 41, 587-591.
  18. Park, S.H., Oh, H.W., Kwon, H.R., Seo, M.J., Yu, Y.M., Youn, Y.N., 2014. Insecticidal Activities of Tussilago farfara Extracts against Culex pipienspallens and Tetranychus urticae. KJOAS 41, 177-185.
  19. Peterson, C., Coats J.R., 2001. Insect repellents - past, present and future. Pesticide Outlook. 12, 154-158. https://doi.org/10.1039/b106296b
  20. Rozendaal, J.A., 1997. Vector control. WHO, Geneva, pp. 7-177.
  21. Rueda, L.M., Rutledge, L.C., Gupta, R.K., 1998. Effect of skin abrasion on the efficacy of the repellent deet against Aedes aegypti. J. Am. Mosq. Control Assoc. 14, 178-182.
  22. Schmutterer, H., 1990. Properties and potential of natural pesticides from the neem tree, Azadirachta indica. Ann. Rev. Entomol. 35, 271-279. https://doi.org/10.1146/annurev.en.35.010190.001415
  23. Shaema, V.P., Ansari, M.A., Razdan, R.K., 1993. Mosquito repellent action of neem (Azadirachta indica) oil. J. AM. Mosq. Control Assoc. 9, 359-360.
  24. Uenoyama, R., Miyazaki, T., Hurst, J.L., Beynon, R.J., Adachi, M., Murooka, T., Onoda, I., Miyazawa, Y., Katayama, R., Yamashita, T., Kaneko, S., Nishikawa, T., Miyazaki, M., 2021. The characteristic response of domestic cats to plant iridoids allows them to gain chemical defense against mosquitoes. Sci. Adv. 20, 7-10.
  25. Wada, Y., Oda, T., Mogi, M., Mori, A., Omori, N., Fukumi, H., Hayashi, K., Mifune, K., Shichijo, A., Matsuo. S., 1975. Ecology of Japanese encephalitis virus in Japan. II. The population of vector mosquitoes and the epidemic of Japanese encephalitis. Trop. Med. 17, 111-127.
  26. Wang, M., Cheng, K.W., Wu, Q., Simon, J.E., 2007. Quantification of nepetalactones in catnip (Nepeta cataria L.) by HPLC coupled with ultraviolet and mass spectrometric detection. Phytochem. Anal. 18, 157-160. https://doi.org/10.1002/pca.965
  27. White, G.B., 1989. Geographical distribution of arthropod-borne diseases and their principal vectors. 1. MALARIA, WHO/VBC/89.967, 7-20.
  28. Zhu, J., Zeng, X., 2006. Adult repellency and larvicidal activity of five plant essential oils against mosquitoes. J. Am. Mosq. Control Assoc. 22, 515-522. https://doi.org/10.2987/8756-971X(2006)22[515:ARALAO]2.0.CO;2