Journal of Microbiology and Biotechnology

The Korean Society for Microbiology and Biotechnology (한국미생물·생명공학회)
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Evaluation of the EtOAc Extract of Lemongrass (Cymbopogon citratus) as a Potential Skincare Cosmetic Material for Acne Vulgaris

  • Kim, Chowon (Department of Food Science and Nutrition, College of Human Ecology, Pusan National University) ;
  • Park, Jumin (Department of Food Science and Nutrition, College of Human Ecology, Pusan National University) ;
  • Lee, Hyeyoung (Division of Applied Bioengineering, Dong-Eui University) ;
  • Hwang, Dae-Youn (Department of Biomaterials Science, Pusan National University) ;
  • Park, So Hae (Department of Biomaterials Science, Pusan National University) ;
  • Lee, Heeseob (Department of Food Science and Nutrition, College of Human Ecology, Pusan National University)
  • Received : 2022.01.28
  • Accepted : 2022.04.18
  • Published : 2022.05.28
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Abstract

This study evaluated the biological properties of lemongrass (Cymbopogon citratus) extracts. The EtOAc extract of lemongrass had DPPH, TEAC, and nitric oxide-scavenging activity assay results of 58.06, 44.14, and 41.08% at the concentration of 50, 10, and 50 ㎍/ml, respectively. The EtOAc extract had higher elastase and collagenase inhibitory activities than the 80% MeOH, n-hexane, BuOH, and water extracts and comparable whitening activity toward monophenolase or diphenolase. Also, the EtOAc fraction had higher lipase inhibitory and antimicrobial activities against Cutibacterium acnes among extracts which is known to an important contributor to the progression of inflammatory acne vulgaris, and an opportunistic pathogen present in human skin. Total phenolic and flavonoid concentrations in the EtOAc extract were 132.31 mg CAE/g extract and 104.50 mg NE/g extract, respectively. Biologically active compounds in lemongrass extracts were analyzed by LC-MS. This study confirms that lemongrass extracts have potential use as cosmetic skincare ingredients. Thus, lemongrass can be considered a promising natural source of readily available, low-cost extracts rich in antioxidant, skincare, and antimicrobial compounds that might be suitable for replacing synthetic compounds in the cosmeceutical industry.

Keywords

Acknowledgement

This work was supported by the BK21 FOUR Program through the National Research Foundation of Korea (NRF) funded by the Ministry of Education, Korea (Grant number F21YY8109033).

References

  1. Yi MR, Kang CH, Bu HJ. 2017. Acetic acid fermentation properties and antioxidant activity of lemongrass vinegar. Kor. J. Food Preserv. 24: 680-687. https://doi.org/10.11002/KJFP.2017.24.5.680
  2. Chae IG, Kim HJ, Yu MH, Kim HI, Lee IS. 2010. Antioxidant and antibacterial activity of commercially available herbs in Korean markets. J. Kor. Soc. Food Sci. Nutr. 39: 1411-1417. https://doi.org/10.3746/JKFN.2010.39.10.1411
  3. Tzortzakis NG, Economakis CD. 2007. Antifungal activity of lemongrass (Cympopogon citratus L.) essential oil against key postharvest pathogens. Innov. Food Sci. Emerg. Technol. 8: 253-258. https://doi.org/10.1016/j.ifset.2007.01.002
  4. Maheswari RU, EuginAmala V. 2015. Analyzing and determining the activity of antimicrobial, functional group and phytochemicals of Cymbopogon citratus using well Diffusion, FT-IR and HPLC. Alcohol 3403: 3468-3487.
  5. Yoo KM, Lee CH, Lee H, Moon B, Lee CY. 2008. Relative antioxidant and cytoprotective activities of common herbs. Food Chem. 106: 929-936. https://doi.org/10.1016/j.foodchem.2007.07.006
  6. Cheel J, Theoduloz C, Rodriguez J, Schmeda-Hirschmann G. 2005. Free radical scavengers and antioxidants from Lemongrass (Cymbopogon citratus (DC.) Stapf.). J. Agric. Food Chem. 53: 2511-2517. https://doi.org/10.1021/jf0479766
  7. Ming LC, Figueiredo RO, Machado SR, Andrade RMC. 1995. Yield of essential oil of and citral content in different parts of lemongrass leaves (Cymbopogon citratus (dc) stape) poaceae. Acta Hortic. 426: 555-559. https://doi.org/10.17660/actahortic.1996.426.60
  8. Miean KH, Mohamed S. 2001. Flavonoid (myricetin, quercetin, kaempferol, luteolin, and apigenin) content of edible tropical plants. J. Agric. Food Chem. 49: 3106-3112. https://doi.org/10.1021/jf000892m
  9. Khadri A, Neffati M, Smiti S, Fale P, Lino ARL, Serralheiro MLM, et al. 2010. Antioxidant, antiacetylcholinesterase and antimicrobial activities of Cymbopogon schoenanthus L. Spreng (lemon grass) from Tunisia. LWT-Food Sci. Technol. 43: 331-336. https://doi.org/10.1016/j.lwt.2009.08.004
  10. Syed M, Khalid MR, Chaudhary FM. 1990. Essential oils of Gramineae family having antibacterial activity. Part-1.(Cymbopogon citratus, C. martinii and C. jawarancusa oils). Pakistan J. Sci. Ind. Res. 33: 529-531.
  11. Ibrahim D. 1992. Antimicrobial activity of the essential oil of the local serai, Cymbopogon citratus. J. Biosci. 3: 87-90.
  12. Baratta MT, Dorman HD, Deans SG, Figueiredo AC, Barroso JG, Ruberto G. 1998. Antimicrobial and antioxidant properties of some commercial essential oils. Flavour Fragr. J. 13: 235-244. https://doi.org/10.1002/(SICI)1099-1026(1998070)13:4<235::AID-FFJ733>3.0.CO;2-T
  13. Tanase C, Bara CI, Popa VI. 2015. Cytogenetical effect of some polyphenol compounds separated from industrial by-products on maize (Zea Mays L.) plants. Cellul. Chem. Technol. 49: 799-805.
  14. Tepe, B., Sokmen, M., Akpulat, H. A., & Sokmen, A. 2006. Screening of the antioxidant potentials of six salvia species from Turkey. Food Chem. 95: 200-204. https://doi.org/10.1016/j.foodchem.2004.12.031
  15. Sujarwo W, Keim AP. 2019. Spondias pinnata (L. f.) Kurz. (Anacardiaceae): Profiles and Applications to Diabetes, pp. 395-405. In Watson RR, Preedy VR (eds.), Bioactive Food as Dietary Interventions for Diabetes, 2nd Ed. Academic Press, London, UK.
  16. Beckman JS, Koppenol WH. 1996. Nitric oxide, superoxide, and peroxynitrite: the good, the bad, and ugly. Am. J. Physiol. Cell Physiol. 271: C1424-C1437. https://doi.org/10.1152/ajpcell.1996.271.5.c1424
  17. Pacher P, Beckman JS, Liaudet L. 1996. Nitric oxide and peroxynitrite: in health and disease. Physiol. Rev. 87: 315-424. https://doi.org/10.1152/physrev.00029.2006
  18. Shin KH, Lee HS, Kim KC. 2008. Sebum creation and acne. J. Skin Barrier Res. 10: 56-60.
  19. Dreno B, Pecastaings S, Corvec S, Veraldi S, Khammari A, Roques C. 2018. Cutibacterium acnes (Propionibacterium acnes) and acne vulgaris: a brief look at the latest updates. J. Eur. Acad. Dermatol. Venereol. 32: 5-14. https://doi.org/10.1111/jdv.15043
  20. Rosenthal M, Goldberg D, Aiello A, Larson E, Foxman B. 2011. Skin microbiota: microbial community structure and its potential association with health and disease. Infect. Genet. Evol. 11: 839-848. https://doi.org/10.1016/j.meegid.2011.03.022
  21. Christensen GJM, Bruggemann H. 2014. Bacterial skin commensals and their role as host guardians. Benef. Microbes 5: 201-215. https://doi.org/10.3920/BM2012.0062
  22. Blois MS. 1958. Antioxidant determinations by the use of a stable free radical. Nature 181: 1199-1200. https://doi.org/10.1038/1811199a0
  23. Re R, Pellegrini N, Proteggente A, Pannala A, Yang M, Rice-Evans C. 1999. Antioxidant activity applying an improved ABTS radical cation decolorization assay. Free Rad. Biol. Med. 26: 1231-1237. https://doi.org/10.1016/S0891-5849(98)00315-3
  24. Jaiswal YS, Tatke PA, Gabhe SY, Ashok V. 2010. Antioxidant activity of various extracts of leaves of Anacardium occidentale (cashew). Res. J. Pharm. Biol. Chem. Sci. 1: 112-119.
  25. Cannell JJ. 1988. Nationally normed elementary achievement testing in America's public schools: how all 50 states are above the national average. Educ. Meas. 7: 5-9. https://doi.org/10.1111/j.1745-3992.1988.tb00424.x
  26. Wunsch E, Heidrich HG. 1963. Zur quantitativen bestimmung der kollagenase. Hoppe-Seylers Z. Physiol. 333: 149-151. https://doi.org/10.1515/bchm2.1963.333.1.149
  27. Kubo I, Kinst-Hori I. 1999. Flavonols from saffron flower: tyrosinase inhibitory activity and inhibition mechanism. J. Agric. Food Chem. 47: 4121-4125. https://doi.org/10.1021/jf990201q
  28. Kumaran A, Karunakaran RJ. 2007. In vitro antioxidant activities of methanol extracts of five Phyllanthus species from India. LWT-Food Sci. Technol. 40: 344-352. https://doi.org/10.1016/j.lwt.2005.09.011
  29. Chang CC, Yang MH, Wen HM, Chern JC. 2002. Estimation of total flavonoid content in propolis by two complementary colorimetric methods. J. Food Drug Anal. 10: 178-182.
  30. De Beer EJ, Sherwood MB. 1945. The paper-disc agar-plate method for the assay of antibiotic substances. J. Bacteriol. 50: 459-467. https://doi.org/10.1128/jb.50.4.459-467.1945
  31. Hammer KA, Carson CF, Riley TV. 1996. Susceptibility of transient and commensal skin flora to the essential oil of Melaleuca alternifolia (tea tree oil). Am. J. Infect. Control 24: 186-189. https://doi.org/10.1016/S0196-6553(96)90011-5
  32. Inui S, Aoshima H, Ito M, Kokubo K, Itami S. 2012. Inhibition of sebum production and Propionibacterium acnes lipase activity by fullerenol, a novel polyhydroxylated fullerene: potential as a therapeutic agent for acne. J. Cosmet. Sci. 63: 259-265.
  33. Boeira CP, Piovesan N, Soquetta MB, Flores DCB, Lucas BN, Rosa CSD, et al. 2018. Extraction of bioactive compounds of lemongrass, antioxidant activity and evaluation of antimicrobial activity in fresh chicken sausage. Cienc. Rural 48: e20180477.
  34. Tiwari M, Dwivedi UN, Kakkar P. 2010. Suppression of oxidative stress and pro-inflammatory mediators by Cymbopogon citratus D. Stapf extract in lipopolysaccharide stimulated murine alveolar macrophages. Food Chem. Toxicol. 48: 2913-2919. https://doi.org/10.1016/j.fct.2010.07.027
  35. Mirghani MES, Liyana Y, Parveen J. 2012. Bioactivity analysis of lemongrass (Cymbopogan citratus) essential oil. Int. Food Res. J. 19: 569-575.
  36. Choi YJ, Hwang KH. 2013. Radical scavenging activities of fruits of Crataegus pinnatifida BUNGE major. from Korea. Nat. Prod. Sci. 19: 186-191.
  37. Antonicelli F, Bellon G, Debelle L, Hornebeck W. 2007. Elastin-elastases and inflamm-aging. Curr. Top. Dev. Biol. 79: 99-155. https://doi.org/10.1016/S0070-2153(06)79005-6
  38. Christensen GJM, Bruggemann H. 2014. Bacterial skin commensals and their role as host guardians. Benef. Microbes 5: 201-215. https://doi.org/10.3920/BM2012.0062
  39. Karim AA, Azlan A, Ismail A, Hashim P, Abd Gani SS, Zainudin BH, et al. 2014. Phenolic composition, antioxidant, anti-wrinkles and tyrosinase inhibitory activities of cocoa pod extract. BMC Complement. Altern. Med. 14: 381. https://doi.org/10.1186/1472-6882-14-381
  40. Kumar GS, Jayaveera KN, Kumar CK, Sanjay UP, Swamy BM, Kumar DV. 2007. Antimicrobial effects of Indian medicinal plants against acne-inducing bacteria. Trop. J. Pharm. Res. 6: 717-723.
  41. Unno M, Cho O, Sugita T. 2017. Inhibition of Propionibacterium acnes lipase activity by the antifungal agent ketoconazole. Microbiol. Immunol. 61: 42-44. https://doi.org/10.1111/1348-0421.12464
  42. El Gharras H. 2009. Polyphenols: food sources, properties and applications-a review. Int. J. Food Sci. Technol. 44: 2512-2518. https://doi.org/10.1111/j.1365-2621.2009.02077.x
  43. Peisner-Feinberg ES, Burchinal MR, Clifford RM, Culkin ML, Howes C, Kagan SL, et al. 2001. The relation of preschool child-care quality to children's cognitive and social developmental trajectories through second grade. Child Dev. 72: 1534-1553. https://doi.org/10.1111/1467-8624.00364
  44. Zhang Y, Shen JF, Yu ZY, Lu BY, Lou DD. 2004. Primary studies on bamboo leaf flavonoids used as anti-aging factor for skin protection. Chem. Ind. Forest Prod. 24: 95-100.
  45. Sarker U, Oba S. 2020. Polyphenol and flavonoid profiles and radical scavenging activity in leafy vegetable Amaranthus gangeticus. BMC Plant Biol. 20: 499. https://doi.org/10.1186/s12870-020-02700-0
  46. Jalali O, Best M, Wong A, Schaeffer B, Bauer B, Johnson L. 2020. Protocatechuic acid as a topical antimicrobial for surgical skin antisepsis. JB JS Open Access 5: e19.00079. https://doi.org/10.2106/JBJS.OA.19.00079