Journal of Microbiology and Biotechnology

The Korean Society for Microbiology and Biotechnology (한국미생물·생명공학회)
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Caenimonas aquaedulcis sp. nov., Isolated from Freshwater of Daechung Reservoir during Microcystis Bloom

  • Le, Ve Van (Cell factory Research Centre, Korea Research Institute of Bioscience and Biotechnology) ;
  • Ko, So-Ra (Cell factory Research Centre, Korea Research Institute of Bioscience and Biotechnology) ;
  • Lee, Sang-Ah (Environmental Safety Groups, Korea Institute of Science and Technology (KIST) Europe) ;
  • Kang, Mingyeong (Cell factory Research Centre, Korea Research Institute of Bioscience and Biotechnology) ;
  • Oh, Hee-Mock (Cell factory Research Centre, Korea Research Institute of Bioscience and Biotechnology) ;
  • Ahn, Chi-Yong (Cell factory Research Centre, Korea Research Institute of Bioscience and Biotechnology)
  • Received : 2022.01.20
  • Accepted : 2022.03.23
  • Published : 2022.05.28
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Abstract

A Gram-stain-negative, white-coloured, and rod-shaped bacterium, strain DR4-4T, was isolated from Daechung Reservoir, Republic of Korea, during Microcystis bloom. Strain DR4-4T was most closely related to Caenimonas terrae SGM1-15T and Caenimonas koreensis EMB320T with 98.1% 16S rRNA gene sequence similarities. The average nucleotide identity (ANI) and digital DNA-DNA hybridization (dDDH) values between strain DR4-4T and closely related type strains were below 79.46% and 22.30%, respectively. The genomic DNA G+C content was 67.5%. The major cellular fatty acids (≥10% of the total) were identified as C16:0, cyclo C17:0, summed feature 3 (C16:1ω7c and/or C16:1ω6c), and summed feature 8 (C18:1ω7c and/or C18:1ω6c). Strain DR4-4T possessed phosphatidylethanolamine, diphosphatidylglycerol, and phosphatidylglycerol as the main polar lipids and Q-8 as the respiratory quinone. The polyamine profile was composed of putrescine, cadaverine, and spermidine. The results of polyphasic characterization indicated that the isolated strain DR4-4T represents a novel species within the genus Caenimonas, for which the name Caenimonas aquaedulcis sp. nov. is proposed. The type strain is DR4-4T (=KCTC 82470T =JCM 34453T).

Keywords

Acknowledgement

We thank Professor Aharon Oren for his expert advice concerning the genus and species epithet and Latin etymology. This study was supported by National Research Foundation of Korea (2019R1A2C2007038 and 2021R1A2C1005151) and the Korea Research Institute of Bioscience and Biotechnology (KRIBB) Research Initiative Program.

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