한국초지조사료학회지 (Journal of The Korean Society of Grassland and Forage Science)

  • 제42권2호
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  • Pages.61-72
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  • 2022
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  • 2287-5824(pISSN)
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  • 2287-5832(eISSN)
한국초지조사료학회 (The Korean Society of Grassland and Forage Science)
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옥수수 사일리지와 대두 사일리지의 혼합급여가 In Vitro 반추위 발효성상 및 거세한우의 성장과 육질등급에 미치는 영향

Effect of Corn Silage and Soybean Silage Mixture on Rumen Fermentation Characteristics In Vitro, and Growth Performance and Meat Grade of Hanwoo Steers

  • Kang, Juhui (Department of Animal Science and Biotechnology, Kyungpook National University) ;
  • Lee, Kihwan (Department of Animal Science and Biotechnology, Kyungpook National University) ;
  • Marbun, Tabita Dameria (Department of Animal Science and Biotechnology, Kyungpook National University) ;
  • Song, Jaeyong (Department of Animal Science, Kyungpook National University) ;
  • Kwon, Chan Ho (Department of Animal Science and Biotechnology, Kyungpook National University) ;
  • Yoon, Duhak (Department of Animal Science and Biotechnology, Kyungpook National University) ;
  • Seo, Jin-Dong (School of Applied Biosciences, Kyungpook National University) ;
  • Jo, Young Min (Gunwi Livestock Cooperative Federation) ;
  • Kim, Jin Yeoul (Gunwi Livestock Cooperative Federation) ;
  • Kim, Eun Joong (Department of Animal Science and Biotechnology, Kyungpook National University)
  • 투고 : 2022.03.17
  • 심사 : 2022.05.23
  • 발행 : 2022.06.30
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초록

옥수수사일리지와 대두 사일리지를 다양한 수준으로 혼합한 사일리지의 in vitro 반추위 발효성상을 평가하는 실험(실험 1)에서 가스발생량은 대두 사일리지의 혼합비율이 증가할수록 배양 48시간을 제외한 모든 발효시간에서 증가하는 경향을 보였다. 암모니아태 질소의 발생량은 배양 24시간에 대두 사일리지 대체율이 증가할수록 높게 나타났다(p<0.05). 건물소화율은 배양 6시간을 제외한 전 배양시간에서 대두 사일리지의 첨가량이 감소함에 따라 낮은 경향을 보였고, acetate와 propionate를 비롯한 총 휘발성 지방산 생성량은 대두 사일리지의 함량이 증가할수록 증가하는 경향을 보였다. 두번째 실험인 옥수수와 대두 혼합사일리지(4%, 원물기준)를 급여한 육성기 거세한우 사양실험에서 육성기에 옥수수-대두 혼합사일리지 급여구의 증체량과 사료효율이 대조구 및 옥수수 사일리지 처리구와 비교하여 높았다(p<0.05). 또한, 육질등급은 처리구별 유의차가 없었지만 옥수수-대두 혼합사일리지의 급여구에서 가장 우수한 육질등급을 나타내었다. 이상의 결과를 종합하면 옥수수 사일리지에 대두 사일리지의 첨가는 반추위 발효에 긍정적인 영향을 미치는 것으로 나타났다. 또한, 옥수수 사일리지를 급여하는 육성기 거세 한우에게 4% 수준의 대두 사일리지 혼합급여는 증체량 및 사료효율에 영향을 미치며, 육질등급의 증가로 고급육 생산에 도움이 될 것으로 판단된다.

The present study was conducted to examine the effect of soybean silage as a crude protein supplement for corn silage in the diet of Hanwoo steers. The first experiment was conducted to evaluate the effect of replacing corn silage with soybean silage at different levels on rumen fermentation characteristics in vitro. Commercially-purchased corn silage was replaced with 0, 4, 8, or 12% of soybean silage. Half gram of the substrate was added to 50 mL of buffer and rumen fluid from Hanwoo cows, and then incubated at 39℃ for 0, 3, 6, 12, 24, and 48 h. At 24 h, the pH of the control (corn silage only) was lower (p<0.05) than that of soybean-supplemented silages, and the pH numerically increased along with increasing proportions of soybean silage. Other rumen parameters, including gas production, ammonia nitrogen, and total volatile fatty acids, were variable. However, they tended to increase with increasing proportions of soybean silage. In the second experiment, 60 Hanwoo steers were allocated to one of three dietary treatments, namely, CON (concentrate with Italian ryegrass), CS (concentrate with corn silage), CS4% (concentrate with corn silage and 4% of soybean silage). Animals were offered experimental diets for 110 days during the growing period and then finished with typified beef diets that were commercially available to evaluate the effect of soybean silage on animal performance and meat quality. With the soybean silage, the weight gain and feed efficiency of the animal were more significant than those of the other treatments during the growing period (p<0.05). However, the dietary treatments had little effect on meat quality except for meat color. In conclusion, corn silage mixed with soybean silage even at a lower level provided a greater ruminal environment and animal performances, particularly with increased carcass weight and feed efficiency during growing period.

키워드

과제정보

본 결과물은 농림축산식품부의 재원으로 농림식품기술기획평가원(IPET)의 고부가가치식품기술개발사업의 지원을 받아 수행되었음(과제번호: 314024-3). 또한, 주저자인 강주희(2017년)의 석사학위 논문 "옥수수와 콩 혼합 사일리지의 in vitro 반추위 분해율과 한우의 성장 및 육질등급에 미치는 영향"의 내용을 일부 수정, 보완한 것임. 저자 일동은 가축의 사양관리에 참여하신 모든 분들께 그리고 군위축산업협동조합 관계자 여러분께 감사의 말씀을 드립니다.

참고문헌

  1. Ahn, B.H., Song, S.C. and Lyu, J.S. 2002. Effects of proportion of roughages and concentrates on growth and carcass characteristics of Hanwoo steers. Journal of Animal Science and Technology. 44(6):747-756. doi:10.5187/JAST.2002.44.6.747
  2. Allen, M., Ford, S., Harrison, J., Hunt, C., Lauer, J., Muck, R. and Soderlund, S. 1995. Corn silage production, management and feeding. American Society of Agronomy. pp. 1-4.
  3. AOAC. 1995. Official Methods of Analysis (16th ed.). Association of Official Analytical Chemists. Washington DC. USA.
  4. Bal, M.A., Shaver, R.D., Jirovec, A.G., Shinners, K.J. and Coors, J.G. 2000. Crop processing and chop length of corn silage: Effects on intake, digestion, and milk production by dairy cows. Journal of Dairy Science. 83(6):1264-1273. doi:10.3168/jds.S0022-0302(00)74993-9
  5. Buxton, D.R., Muck, R.E., Harrison, J.H., Albrecht, K.A. and Beauchemin, K.A. 2003. Alfalfa and other perennial legume silage. In: D.R. Buxton, R.E. Muck and J.H. Harrison (Ed.), Silage Science and Technology. American Society of Agronomy, Inc., Crop Science Society of America, Inc., Soil Science Society of America, Inc. pp. 633-664.
  6. Carruthers, K., Prithiviraj, B., Fe, Q., Cloutier, D., Martin, R.C. and Smith, D.L. 2000. Intercropping of corn with soybean, lupin and forages: Silage yield and quality. Journal of Agronomy and Crop Science. 185(3):177-185. doi:10.1046/j.1439-037x.2000.00421.x
  7. Chaney, A.L. and Marbach, E.P. 1962. Modified reagents for determination of urea and ammonia. Clinical Chemistry. 8(2):130-132. doi:10.1093/clinchem/8.2.130
  8. Collins, M. and Newman, Y.C. 2008. Forage quality. In: M. Collins, C.J. Nelson, K.J. Moore and R.F. Barnes (Eds.), Forages Volume 1. An Introduction to Grassland Agriculture. Wiley Blackwell. pp. 269-286.
  9. Davies, Z.S., Mason, D., Brooks, A.E., Griffith, G.W., Merry, R.J. and Theodorou, M.K. 2000. An automated system for measuring gas production from forages inoculated with rumen fluid and its use in determining the effect of enzymes on grass silage. Animal Feed Science and Technology. 83(3):205-221. doi:10.1016/S0377-8401(99)00138-8
  10. Erwin, E.S., Marco, G.J. and Emery, E.M. 1961. Volatile fatty acid analyses of blood and rumen fluid by gas chromatography. Journal of Dairy Science. 44(9):1768-1771. https://doi.org/10.3168/jds.s0022-0302(61)89956-6
  11. Getachew, G., Robinson, P.H., DePeters, E.J. and Taylor, S.J. 2004. Relationships between chemical composition, dry matter degradation and in vitro gas production of several ruminant feeds. Animal Feed Science and Technology. 111(1):57-71. doi:10.1016/S0377-8401(03)00217-7
  12. Ghizzi, L.G., Del Valle, T.A., Zilio, E.M.C., Sakamoto, L.Y., Marques, J.A., Dias, M.S.S., Nunes, A.T., Gheller, L.S., De P. Silva, T.B., Grigoletto, N.T.S., Takiya, C.S., Da Silva, G.G. and Renno, F.P. 2020. Partial replacement of corn silage with soybean silage on nutrient digestibility, ruminal fermentation, and milk fatty acid profile of dairy cows. Animal Feed Science and Technology. 266:114526. doi:10.1016/j.anifeedsci.2020.114526
  13. Grummer, R.R., Clark, J.H., Davis, C.L. and Murphy, M.R. 1984. Effect of ruminal ammonia-nitrogen concentration on protein degradation in situ. Journal of Dairy Science. 67(10):2294-2301. doi:10.3168/jds.S0022-0302(84)81577-5
  14. Hall, M.B. and Mertens, D.R. 2008. In vitro fermentation vessel type and method alter fiber digestibility estimates. Journal of Dairy Science. 91(1):301-307. doi:10.3168/jds.2006-689
  15. Hoover, W.H. 1986. Chemical factors involved in ruminal fiber digestion. Journal of Dairy Science. 69(10):2755-2766. doi:10.3168/jds.S0022-0302(86)80724-X
  16. IBM Corporation. 2015. IBM SPSS Statistics for Windows, Version 23.0. IBM Corporation. Armonk. NY.
  17. Jin, Y.H., Jin, M.G., Hong, J.S., Lee, H.G., Lee, B.K., Kim, J.S. and Choi, Y.J. 2002. Effect of cubed roughage supplementation and red clay levels on growth performance, meat quality and economic benefits in Korean native cattle. Journal of Animal Science and Technology. 44(1):61-68. doi:10.5187/JAST.2002.44.1.061
  18. Kang, J., Song, J., Marbun, T.D., Kwon, C.H. and Kim, E.J. 2017. Effect of intercropped corn and soybean silage on nutritive values, in vitro ruminal fermentation, and milk production of Holstein dairy cows. Journal of the Korean Society of Grassland and Forage Science. 37(3):216-222. doi:10.5333/KGFS.2017.37.3.216
  19. Kang, S.W., Jeong, H.Y., Ahn, B.S., Oh, Y.K. and Son, Y.S. 2004. Effects of feeding type of concentrates during growing period and slaughter age on growth performance, feed efficiency and carcass characteristics in growing-fattening Holstein steers. Journal of Animal Science and Technology. 46(6):989-998. doi:10.5187/JAST.2004.46.6.989
  20. Kim, B.K., Oh, D.Y., Hwang, E.G., Song, Y.H., Lee, S.O., Jung, K.K. and Ha, J.J. 2013. The effects of different crude protein levels in the concentrates on carcass and meat quality characteristics of Hanwoo steers. Journal of Animal Science and Technology. 55(1):61-66. doi:10.5187/JAST.2013.55.1.61
  21. Kim, J.G., Cheong, E.C., Li, Y.F., Kim, H.J., Farhad, A. and Kim, M.J. 2021. Effect of TMR feed mixed with whole crop rice on growth performance and meat quality of Hanwoo steers. Journal of the Korean Society of Grassland and Forage Science. 41(4):267-272. doi:10.5333/KGFS.2021.41.4.267
  22. Kim, S.I., Jung, K.K., Kim, J.Y., Lee, S.W., Baek, K.H. and Choi, C.B. 2007. Effect of feeding high quality hay on performance and physico-chemical characteristics of carcass of Hanwoo steers. Journal of Animal Science and Technology. 49(6):783-800. doi:10.5187/JAST.2007.49.6.783
  23. Kim, W.H., Seo, S., Shin, J.S., Lim, Y.C., Kim, K.Y., Jung, M.W. and Kim, T.H. 2006. Effect of seeding date and rate on the agronomic characteristics and yield of forage barley. Journal of the Korean Society of Grassland Science. 26(3):155-158. https://doi.org/10.5333/KGFS.2006.26.3.155
  24. Korea Institute for Animal Products Quality Evaluation. 2014. Animal product rating standard in detail. Notification No. 2014-4. Ministry of Agriculture, Food and Rural Affairs, Republic of Korea. https://www.ekape.or.kr/english/index.do
  25. Krause, M., Beauchemin, K.A., Rode, L.M., Farr, B.I. and Norgaard, P. 1998. Fibrolytic enzyme treatment of barley grain and source of forage in high-grain diets fed to growing cattle. Journal of Animal Science. 76(11):2912-2920. doi:10.2527/1998.76112912x
  26. Kwon, E.G., Hong, S.K., Seong, H.H., Yun, S.G., Park, B.K., Cho, Y.M., Cho, W.M., Chang, S.S., Shin, K.J. and Paek, B.H. 2005. Effects of ad libitum and restricted feeding of concentrates on body weight gain, feed intake and blood metabolites of Hanwoo steers at various growth stages. Journal of Animal Science and Technology. 47(5):745-758. doi:10.5187/JAST.2005.47.5.745
  27. Marbun, T.D., Lee, K., Song, J., Kwon, C.H., Yoon, D., Lee, S.M., Kang, J., Lee, C., Cho, S. and Kim, E.J. 2020. Effect of lactic acid bacteria on the nutritive value and in vitro ruminal digestibility of maize and rice straw silage. Applied Sciences. 10(21):7801. doi:10.3390/app10217801
  28. Martin, R.C., Astatkie, T. and Cooper, J.M. 1998. The effect of soybean variety on corn-soybean intercrop biomass and protein yields. Canadian Journal of Plant Science. 78(2):289-294. doi:10.4141/P97-030
  29. McDougall, E.I. 1948. Studies on ruminant saliva. 1. The composition and output of sheep's saliva. Biochemical Journal. 43(1):99-109. https://doi.org/10.1042/bj0430099
  30. Murphy, T.A. and Loerch, S.C. 1994. Effects of restricted feeding of growing steers on performance, carcass characteristics, and composition. Journal of Animal Science. 72(9):2497-2507. doi:10.2527/1994.7292497x
  31. Oka, A., Dohgo, T., Ohtagaki, S. and Juen, M. 1999. Effects of roughage levels on growth, beef quality, ruminal contents and serum constituents in Japanese black steers during the growing period. Animal Science Journal. 70(6):451-459. doi:10.2508/chikusan.70.451
  32. Plaizier, J.C., Krause, D.O., Gozho, G.N. and McBride, B.W. 2008. Subacute ruminal acidosis in dairy cows: The physiological causes, incidence and consequences. The Veterinary Journal. 176(1):21-31. doi:10.1016/j.tvjl.2007.12.016
  33. Prithiviraj, B., Carruthers, K., Fe, Q., Cloutier, D., Martin, R.C. and Smith, D.L. 2000. Intercropping of corn with soybean and lupin for silage: Effect of seeding date on yield and quality. Journal of Agronomy and Crop Science. 185(2):129-136. doi:10.1046/j.1439-037x.2000.00418.x
  34. Qu, Y., Jiang, W., Yin, G., Wei, C. and Bao, J. 2013. Effects of feeding corn-lablab bean mixture silages on nutrient apparent digestibility and performance of dairy cows. Asian-Australasian Journal of Animal Sciences. 26(4):509-516. doi:10.5713/ajas.2012.12531
  35. Seo, J.D., Jo, H., Kim, M., Song, J. and Lee, J.D. 2019. Agronomic traits and forage production in a mixed-planting with corn for forage soybean cultivars, Chookdu 1 and Chookdu 2. Plant Breeding and Biotechnology. 7:123-131. doi:10.9787/PBB.2019.7.2.123
  36. Seol, Y.J., Kim, K.H., Baek, Y.C., Lee, S.C., Ok, J.U., Lee, K.Y., Hong, S.K., Jang, S.S., Choi, C.W. and Song, M.K. 2011. Determination of maintenance energy requirements for growing Hanwoo steers. Journal of Animal Science and Technology. 53(2):155-160. doi:10.5187/JAST.2011.53.2.155
  37. Suarez, B.J., Van Reenen, C.G., Stockhofe, N., Dijkstra, J. and Gerrits, W.J.J. 2007. Effect of roughage source and roughage to concentrate ratio on animal performance and rumen development in veal calves. Journal of Dairy Science. 90(5):2390-2403. doi:10.3168/jds.2006-524
  38. Theodorou, M.K., Williams, B.A., Dhanoa, M.S., McAllan, A.B. and France, J. 1994. A simple gas production method using a pressure transducer to determine the fermentation kinetics of ruminant feeds. Animal Feed Science and Technology. 48(3-4):185-197. doi:10.1016/0377-8401(94)90171-6
  39. Tilley, J.M.A. and Terry, R.A. 1963. A two-stage technique for the in vitro digestion of forage crops. Grass and Forage Science. 18(2):104-111. doi:10.1111/j.1365-2494.1963.tb00335.x
  40. Van Soest, P.J., Robertson, J.B. and Lewis, B.A. 1991. Methods for dietary fiber, neutral detergent fiber, and nonstarch polysaccharides in relation to animal nutrition. Journal of Dairy Science. 74(10):3583-3597. doi:10.3168/jds.S0022-0302(91)78551-2
  41. Vargas-Bello-Perez, E., Mustafa, A.F. and Seguin, P. 2008. Effects of feeding forage soybean silage on milk production, nutrient digestion, and ruminal fermentation of lactating dairy cows. Journal of Dairy Science. 91(1):229-235. doi:10.3168/jds.2007-0484
  42. Witzig, M., Lengowski, M.B., Zuber, K.H.R., Mohring, J. and Rodehutscord, M. 2018. Effects of supplementing corn silage with different nitrogen sources on ruminal fermentation and microbial populations in vitro. Anaerobe. 51:99-109. doi:10.1016/j.anaerobe.2018.04.016